PMID- 27775858
OWN - NLM
STAT- MEDLINE
DCOM- 20171005
LR  - 20240325
IS  - 1467-7652 (Electronic)
IS  - 1467-7644 (Print)
IS  - 1467-7644 (Linking)
VI  - 15
IP  - 5
DP  - 2017 May
TI  - A phosphate starvation-driven bidirectional promoter as a potential tool for crop 
      improvement and in vitro plant biotechnology.
PG  - 558-567
LID - 10.1111/pbi.12653 [doi]
AB  - Phosphate (Pi)-deficient soils are a major limitant factor for crop production in 
      many regions of the world. Despite that plants have innovated several 
      developmental and biochemical strategies to deal with this stress, there are 
      still massive extensions of land which combine several abiotic stresses, 
      including phosphate starvation, that limit their use for plant growth and food 
      production. In several plant species, a genetic programme underlies the 
      biochemical and developmental responses of the organism to cope with low 
      phosphate (Pi) availability. Both protein- and miRNA-coding genes involved in the 
      adaptative response are transcriptionally activated upon Pi starvation. Several 
      of the responsive genes have been identified as transcriptional targets of PHR1, 
      a transcription factor that binds a conserved cis-element called PHR1-binding 
      site (P1BS). Our group has previously described and characterized a minimal 
      genetic arrangement that includes two P1BS elements, as a phosphate-responsive 
      enhancer (EZ2). Here, we report the engineering and successful use of a 
      phosphate-dependent bidirectional promoter, which has been designed and 
      constructed based on the palindromic sequences of the two P1BS elements present 
      in EZ2. This bidirectional promoter has a potential use in both plant in vitro 
      approaches and in the generation of improved crops adapted to Pi starvation and 
      other abiotic stresses.
CI  - (c) 2016 The Authors. Plant Biotechnology Journal published by Society for 
      Experimental Biology and The Association of Applied Biologists and John Wiley & 
      Sons Ltd.
FAU - Araceli, Oropeza-Aburto
AU  - Araceli OA
AD  - Metabolic Engineering Laboratory, Unidad de Genomica Avanzada - LANGEBIO 
      CINVESTAV, Irapuato, Guanajuato, Mexico.
FAU - Alfredo, Cruz-Ramirez
AU  - Alfredo CR
AD  - Molecular and Developmental Complexity Laboratory, Unidad de Genomica Avanzada - 
      LANGEBIO CINVESTAV, Irapuato, Guanajuato, Mexico.
FAU - Javier, Mora-Macias
AU  - Javier MM
AD  - Metabolic Engineering Laboratory, Unidad de Genomica Avanzada - LANGEBIO 
      CINVESTAV, Irapuato, Guanajuato, Mexico.
FAU - Luis, Herrera-Estrella
AU  - Luis HE
AD  - Metabolic Engineering Laboratory, Unidad de Genomica Avanzada - LANGEBIO 
      CINVESTAV, Irapuato, Guanajuato, Mexico.
LA  - eng
GR  - 55007646/HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161227
PL  - England
TA  - Plant Biotechnol J
JT  - Plant biotechnology journal
JID - 101201889
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Phosphates)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Arabidopsis/drug effects/*genetics/physiology
MH  - Arabidopsis Proteins/genetics
MH  - Biotechnology/methods
MH  - Crops, Agricultural/*genetics
MH  - Green Fluorescent Proteins/genetics
MH  - Phosphates/*metabolism/pharmacology
MH  - Plant Breeding
MH  - Plants, Genetically Modified
MH  - *Promoter Regions, Genetic
PMC - PMC5398999
OTO - NOTNLM
OT  - bioengeneering
OT  - crop improvement
OT  - enhancer
OT  - phosphate starvation
OT  - roots
EDAT- 2016/10/25 06:00
MHDA- 2017/10/06 06:00
PMCR- 2017/04/21
CRDT- 2016/10/25 06:00
PHST- 2016/07/26 00:00 [received]
PHST- 2016/10/11 00:00 [revised]
PHST- 2016/10/20 00:00 [accepted]
PHST- 2016/10/25 06:00 [pubmed]
PHST- 2017/10/06 06:00 [medline]
PHST- 2016/10/25 06:00 [entrez]
PHST- 2017/04/21 00:00 [pmc-release]
AID - PBI12653 [pii]
AID - 10.1111/pbi.12653 [doi]
PST - ppublish
SO  - Plant Biotechnol J. 2017 May;15(5):558-567. doi: 10.1111/pbi.12653. Epub 2016 Dec 
      27.