PMID- 27775894
OWN - NLM
STAT- MEDLINE
DCOM- 20180817
LR  - 20180817
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 88
IP  - 22
DP  - 2016 Nov 15
TI  - A Book-Type Dried Plasma Spot Card for Automated Flow-Through Elution Coupled 
      with Online SPE-LC-MS/MS Bioanalysis of Opioids and Stimulants in blood.
PG  - 11229-11237
AB  - Despite many benefits including simple point-of-care sample collection, reduced 
      costs, and simplified shipping and storage, dried blood spot (DBS) techniques 
      have faced adoption resistance due to factors such as the hematocrit (Hct) 
      effects and the established preference for bioanalysis of plasma rather than 
      whole blood. One way to potentially circumvent these challenges is to adopt the 
      concept of dried plasma spot (DPS) techniques. One approach to accomplishing this 
      is through an on-card red blood cell (RBC) filtration to generate plasma from 
      whole blood without the need for centrifugation. In this report, a book-type DPS 
      card has been developed and validated by employing fully automated flow-through 
      elution coupled with online SPE-LC-ESI-MS/MS for the quantitative determination 
      of four representative opioids (morphine, codeine, oxycodone, hydrocodone) and 
      five stimulants (amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine 
      (MDMA), phentermine, and mephedrone) in one method using their corresponding 
      deuterium-labeled analogues as internal standards. Method validation results 
      showed good linearity (R(2) >/= 0.9963) ranging from 5 to 1000 ng/mL. Intraday and 
      interday precision and accuracy were within the acceptable limits at four quality 
      control (QC) levels. Extraction recovery was >/=87.9% at both the lower limit of 
      quantitation (LLOQ) and the upper limit of quantitation (ULOQ) along with 
      acceptable selectivity and sensitivity. DPS on-card short-term stability was 
      compound-dependent and storage-dependent. The additional benefits of the 
      validated book-type DPS card include a wider applicability range of Hct (30% to 
      60%), automated online analysis compatibility, and a higher plasma volume yield.
FAU - Ryona, Imelda
AU  - Ryona I
AD  - Q2 Solutions, 19 Brown Road, Ithaca, New York 14850, United States.
FAU - Henion, Jack
AU  - Henion J
AD  - Q2 Solutions, 19 Brown Road, Ithaca, New York 14850, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161102
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Analgesics, Opioid)
SB  - IM
MH  - Analgesics, Opioid/*blood
MH  - Automation
MH  - Chromatography, Liquid/instrumentation
MH  - Dried Blood Spot Testing/instrumentation/*methods
MH  - Humans
MH  - Quality Control
MH  - Tandem Mass Spectrometry/instrumentation
EDAT- 2016/10/25 06:00
MHDA- 2018/08/18 06:00
CRDT- 2016/10/25 06:00
PHST- 2016/10/25 06:00 [pubmed]
PHST- 2018/08/18 06:00 [medline]
PHST- 2016/10/25 06:00 [entrez]
AID - 10.1021/acs.analchem.6b03691 [doi]
PST - ppublish
SO  - Anal Chem. 2016 Nov 15;88(22):11229-11237. doi: 10.1021/acs.analchem.6b03691. 
      Epub 2016 Nov 2.