PMID- 27780127
OWN - NLM
STAT- MEDLINE
DCOM- 20180801
LR  - 20180801
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 173
DP  - 2017 Feb 15
TI  - Highly sensitive turn-on fluorescence detection of thrombomodulin based on 
      fluorescence resonance energy transfer.
PG  - 675-680
LID - S1386-1425(16)30612-6 [pii]
LID - 10.1016/j.saa.2016.10.022 [doi]
AB  - As an integral glycoprotein on the surface of endothelial cells, thrombomodulin 
      (TM) has very high affinity for thrombin. TM has been regarded to be a marker of 
      endothelial damage since it can be released during endothelial cell injury. In 
      this work, a highly sensitive fluorescence method for the quantitative detection 
      of TM was developed. TM antibody (Ab) and bovine serum albumin (BSA) were bound 
      on gold nanoparticles (AuNPs) to construct BSA-AuNPs-Ab nanocomposites and they 
      were characterized by transmission electron microscope and UV-vis 
      spectrophotometry. The fluorescence of acridine orange (AO) was quenched by the 
      prepared gold nanocomposites based on fluorescence resonance energy transfer 
      (FRET). In the presence of TM, the fluorescence was turned on due to the 
      effective separation of AO from the surface of gold nanocomposites. Under optimum 
      conditions, the enhanced fluorescence intensity displayed a linear relationship 
      with the logarithm of the TM concentration from 0.1pgmL(-1) to 5ngmL(-1) with a 
      low detection limit of 12fgmL(-1). The release of soluble thrombomodulin (sTM) by 
      the injured HUVEC-C cells in the presence of H(2)O(2) was investigated using the 
      proposed method. The released sTM content in the growth medium was found to be 
      increased with the enhancement of contact time of the cells with H(2)O(2).
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Kong, Liyan
AU  - Kong L
AD  - Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research 
      (Ministry of Education of China), College of Chemistry and Chemical Engineering, 
      Hunan Normal University, Changsha 410081, PR China.
FAU - Zhu, Jiaming
AU  - Zhu J
AD  - Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research 
      (Ministry of Education of China), College of Chemistry and Chemical Engineering, 
      Hunan Normal University, Changsha 410081, PR China.
FAU - Wang, Wen
AU  - Wang W
AD  - Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research 
      (Ministry of Education of China), College of Chemistry and Chemical Engineering, 
      Hunan Normal University, Changsha 410081, PR China.
FAU - Jin, Lehe
AU  - Jin L
AD  - Department of Chemistry, College of Chemistry and Chemical Engineering, Hunan 
      Normal University, Changsha 410081, PR China.
FAU - Fu, Yanjiao
AU  - Fu Y
AD  - Department of Chemistry, College of Chemistry and Chemical Engineering, Hunan 
      Normal University, Changsha 410081, PR China.
FAU - Duan, Bohui
AU  - Duan B
AD  - Department of Chemistry, College of Chemistry and Chemical Engineering, Hunan 
      Normal University, Changsha 410081, PR China.
FAU - Tan, Liang
AU  - Tan L
AD  - Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research 
      (Ministry of Education of China), College of Chemistry and Chemical Engineering, 
      Hunan Normal University, Changsha 410081, PR China; Department of Chemistry, 
      College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 
      410081, PR China. Electronic address: liangtan@hunnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20161017
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (THBD protein, human)
RN  - 0 (Thrombomodulin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7440-57-5 (Gold)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Antibodies, Monoclonal/chemistry/immunology
MH  - Fluorescence Resonance Energy Transfer/*methods
MH  - Fluorescent Dyes/chemistry
MH  - Gold/*chemistry
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Limit of Detection
MH  - Metal Nanoparticles/*chemistry
MH  - Microscopy, Electron, Transmission
MH  - Sensitivity and Specificity
MH  - Serum Albumin, Bovine
MH  - Spectrophotometry, Ultraviolet
MH  - Thrombomodulin/*analysis/immunology
OTO - NOTNLM
OT  - Acridine orange
OT  - Fluorescence resonance energy transfer
OT  - Gold nanocomposites
OT  - Immunoreaction
OT  - Thrombomodulin
EDAT- 2016/10/26 06:00
MHDA- 2018/08/02 06:00
CRDT- 2016/10/26 06:00
PHST- 2016/07/13 00:00 [received]
PHST- 2016/10/01 00:00 [revised]
PHST- 2016/10/16 00:00 [accepted]
PHST- 2016/10/26 06:00 [pubmed]
PHST- 2018/08/02 06:00 [medline]
PHST- 2016/10/26 06:00 [entrez]
AID - S1386-1425(16)30612-6 [pii]
AID - 10.1016/j.saa.2016.10.022 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:675-680. doi: 
      10.1016/j.saa.2016.10.022. Epub 2016 Oct 17.