PMID- 27782046
OWN - NLM
STAT- MEDLINE
DCOM- 20170913
LR  - 20240326
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 8
IP  - 10
DP  - 2016 Oct 4
TI  - A Viral Noncoding RNA Complements a Weakened Viral RNA Silencing Suppressor and 
      Promotes Efficient Systemic Host Infection.
LID - 272
AB  - Systemic movement of beet necrotic yellow vein virus (BNYVV) in Beta macrocarpa 
      depends on viral RNA3, whereas in Nicotiana benthamiana this RNA is dispensable. 
      RNA3 contains a coremin motif of 20 nucleotides essential for the stabilization 
      of noncoding RNA3 (ncRNA3) and for long-distance movement in Beta species. 
      Coremin mutants that are unable to accumulate ncRNA3 also do not achieve systemic 
      movement in Beta species. A mutant virus carrying a mutation in the p14 viral 
      suppressor of RNA silencing (VSR), unable to move long distances, can be 
      complemented with the ncRNA3 in the lesion phenotype, viral RNA accumulation, and 
      systemic spread. Analyses of the BNYVV VSR mechanism of action led to the 
      identification of the RNA-dependent RNA polymerase 6 (RDR6) pathway as a target 
      of the virus VSR and the assignment of a VSR function to the ncRNA3.
FAU - Flobinus, Alyssa
AU  - Flobinus A
AD  - Institut de Biologie Moleculaire des Plantes, Integrative Virology, CNRS UPR2367, 
      Universite de Strasbourg, 12 rue du General Zimmer, 67084 Strasbourg, France. 
      kamalhleibieh@gmail.com.
FAU - Hleibieh, Kamal
AU  - Hleibieh K
AD  - Institut de Biologie Moleculaire des Plantes, Integrative Virology, CNRS UPR2367, 
      Universite de Strasbourg, 12 rue du General Zimmer, 67084 Strasbourg, France. 
      kamalhleibieh@gmail.com.
FAU - Klein, Elodie
AU  - Klein E
AD  - Institut de Biologie Moleculaire des Plantes, Integrative Virology, CNRS UPR2367, 
      Universite de Strasbourg, 12 rue du General Zimmer, 67084 Strasbourg, France. 
      kamalhleibieh@gmail.com.
AD  - SESVanderHave, Industriepark soldatenplein, Z2nr15, Tienen B3300, Belgium. 
      kamalhleibieh@gmail.com.
FAU - Ratti, Claudio
AU  - Ratti C
AD  - Dipartimento di Scienze Agrarie, Area Patologia Vegetale, Universita di Bologna, 
      Viale Fanin 40, 40127 Bologna, Italy. claudio.ratti@unibo.it.
FAU - Bouzoubaa, Salah
AU  - Bouzoubaa S
AD  - Institut de Biologie Moleculaire des Plantes, Integrative Virology, CNRS UPR2367, 
      Universite de Strasbourg, 12 rue du General Zimmer, 67084 Strasbourg, France. 
      kamalhleibieh@gmail.com.
FAU - Gilmer, David
AU  - Gilmer D
AD  - Institut de Biologie Moleculaire des Plantes, Integrative Virology, CNRS UPR2367, 
      Universite de Strasbourg, 12 rue du General Zimmer, 67084 Strasbourg, France. 
      gilmer@unistra.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161004
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (RNA, Untranslated)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Chenopodiaceae
MH  - *Gene Silencing
MH  - Genetic Complementation Test
MH  - *Host-Pathogen Interactions
MH  - *Immune Evasion
MH  - Mutation
MH  - Plant Diseases/*virology
MH  - Plant Viruses/*pathogenicity
MH  - RNA, Untranslated/*metabolism
MH  - RNA, Viral/*metabolism
MH  - Nicotiana
PMC - PMC5086608
OTO - NOTNLM
OT  - BNYVV
OT  - RNA silencing suppression
OT  - RSS
OT  - systemic movement
OT  - viral noncoding RNA
COIS- The authors declare no conflict of interest and precise that E.K. is employee of 
      SESVanderHave and provided help in the small RNA assays. SESVanderHave had no 
      role in the design of the study; in the collection, analyses, or interpretation 
      of data; in the writing of the manuscript, or in the decision to publish the 
      results.
EDAT- 2016/10/27 06:00
MHDA- 2017/09/14 06:00
PMCR- 2016/10/01
CRDT- 2016/10/27 06:00
PHST- 2016/07/13 00:00 [received]
PHST- 2016/09/27 00:00 [accepted]
PHST- 2016/10/27 06:00 [pubmed]
PHST- 2017/09/14 06:00 [medline]
PHST- 2016/10/27 06:00 [entrez]
PHST- 2016/10/01 00:00 [pmc-release]
AID - v8100272 [pii]
AID - viruses-08-00272 [pii]
AID - 10.3390/v8100272 [doi]
PST - epublish
SO  - Viruses. 2016 Oct 4;8(10):272. doi: 10.3390/v8100272.