PMID- 27782161
OWN - NLM
STAT- MEDLINE
DCOM- 20180426
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Oct 26
TI  - Maltodextrin enhances biofilm elimination by electrochemical scaffold.
PG  - 36003
LID - 10.1038/srep36003 [doi]
LID - 36003
AB  - Electrochemical scaffolds (e-scaffolds) continuously generate low concentrations 
      of H(2)O(2) suitable for damaging wound biofilms without damaging host tissue. 
      Nevertheless, retarded diffusion combined with H(2)O(2) degradation can limit the 
      efficacy of this potentially important clinical tool. H(2)O(2) diffusion into 
      biofilms and bacterial cells can be increased by damaging the biofilm structure 
      or by activating membrane transportation channels by exposure to hyperosmotic 
      agents. We hypothesized that e-scaffolds would be more effective against 
      Acinetobacter baumannii and Staphylococcus aureus biofilms in the presence of a 
      hyperosmotic agent. E-scaffolds polarized at -600 mV(Ag/AgCl) were overlaid onto 
      preformed biofilms in media containing various maltodextrin concentrations. 
      E-scaffold alone decreased A. baumannii and S. aureus biofilm cell densities by 
      (3.92 +/- 0.15) log and (2.31 +/- 0.12) log, respectively. Compared to untreated 
      biofilms, the efficacy of the e-scaffold increased to a maximum (8.27 +/- 0.05) log 
      reduction in A. baumannii and (4.71 +/- 0.12) log reduction in S. aureus biofilm 
      cell densities upon 10 mM and 30 mM maltodextrin addition, respectively. Overall 
      ~55% decrease in relative biofilm surface coverage was achieved for both species. 
      We conclude that combined treatment with electrochemically generated H(2)O(2) 
      from an e-scaffold and maltodextrin is more effective in decreasing viable 
      biofilm cell density.
FAU - Sultana, Sujala T
AU  - Sultana ST
AD  - School of Chemical Engineering &Bioengineering, Washington State University, 
      Pullman, 99164, WA, USA.
FAU - Call, Douglas R
AU  - Call DR
AD  - Paul G. Allen School for Global Animal Health, Washington State University, 
      Pullman, 99164, WA, USA.
FAU - Beyenal, Haluk
AU  - Beyenal H
AD  - School of Chemical Engineering &Bioengineering, Washington State University, 
      Pullman, 99164, WA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20161026
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Polysaccharides)
RN  - 7CVR7L4A2D (maltodextrin)
SB  - IM
MH  - Acinetobacter baumannii/*physiology
MH  - Biofilms/*growth & development
MH  - *Electrochemical Techniques
MH  - Polysaccharides/*chemistry
MH  - Staphylococcus aureus/*physiology
MH  - Tissue Scaffolds/*chemistry
PMC - PMC5080540
EDAT- 2016/10/27 06:00
MHDA- 2018/04/27 06:00
PMCR- 2016/10/26
CRDT- 2016/10/27 06:00
PHST- 2016/06/17 00:00 [received]
PHST- 2016/09/22 00:00 [accepted]
PHST- 2016/10/27 06:00 [pubmed]
PHST- 2018/04/27 06:00 [medline]
PHST- 2016/10/27 06:00 [entrez]
PHST- 2016/10/26 00:00 [pmc-release]
AID - srep36003 [pii]
AID - 10.1038/srep36003 [doi]
PST - epublish
SO  - Sci Rep. 2016 Oct 26;6:36003. doi: 10.1038/srep36003.