PMID- 27783263
OWN - NLM
STAT- MEDLINE
DCOM- 20170407
LR  - 20221207
IS  - 1573-6814 (Electronic)
IS  - 1389-9333 (Linking)
VI  - 18
IP  - 1
DP  - 2017 Mar
TI  - HLA-typing analysis following allogeneic bone grafting for sinus lifting.
PG  - 75-81
LID - 10.1007/s10561-016-9594-1 [doi]
AB  - According to the Brazilian Association of Organ Transplants, in 2015, 19,408 bone 
      transplants were performed in Brazil, over 90% by Dental Surgeons. The surgical 
      technique itself has a respectable number of reports regarding its clinical 
      efficacy, as measured by long-term survival of dental implants in grafted areas. 
      Uncertainty remains, however, as to whether fresh frozen grafts from human bone 
      donors remain immunologically innocuous in the body of the host. Six male with no 
      previous medical history of note, including systemic diseases, surgery or blood 
      transfusion were selected. These patients underwent reconstructive procedures 
      (sinus lifting) using fresh frozen human bone from a tissue bank. All patients 
      had venous blood samples collected prior to surgery and 6 months after the 
      procedure. Anti-HLA analysis for the detection of HLA (human leukocyte antigen) 
      antibodies was performed using methods such as the LABScreen PRA Class I and 
      Class II, LABScreen Single Antigen Class I and Class II, Luminex Platform. 
      Reactive individuals to the screening tests (LABScreen PRA) were further 
      investigated to determine the specificity of the antibodies detected (LABScreen 
      Single Antigen) with a cutoff value of median fluorescence intensity >/=500. As a 
      result, it was observed that two patients (33%) were positive in screening tests, 
      one presenting with anti-HLA Class I and II sensitization and the other with 
      anti-HLA class II. The specificity analysis showed that the patients sensitized 
      to HLA class II presented 4 specificities, 3 of which immunologically relevant. 
      In the second individual, 23 specificities were identified, 6 of which 
      immunologically important for HLA class I and 4 specificities for HLA class II, 3 
      of these were immunologically important. All specificities detected had average 
      fluorescence. These findings are suggestive that sinus-lifting procedures with 
      allogeneic bone can induce immunological sensitization.
FAU - Piaia, Marcelo
AU  - Piaia M
AD  - Laboratory of Immunology and Molecular Biology, Sao Leopoldo Mandic Institute and 
      Research Center, R. Jose Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil.
FAU - Bub, Carolina Bonet
AU  - Bub CB
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Succi, Guilherme de Menezes
AU  - Succi GM
AD  - Laboratory of Immunology and Molecular Biology, Sao Leopoldo Mandic Institute and 
      Research Center, R. Jose Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil.
FAU - Torres, Margareth
AU  - Torres M
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Costa, Thiago Henrique
AU  - Costa TH
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Pinheiro, Fabricio Costa
AU  - Pinheiro FC
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Napimoga, Marcelo Henrique
AU  - Napimoga MH
AD  - Laboratory of Immunology and Molecular Biology, Sao Leopoldo Mandic Institute and 
      Research Center, R. Jose Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil. 
      marcelo.napimoga@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20161025
PL  - Netherlands
TA  - Cell Tissue Bank
JT  - Cell and tissue banking
JID - 100965121
RN  - 0 (Antibodies)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Aged
MH  - Antibodies/*blood/*immunology
MH  - *Bone Transplantation
MH  - Bone and Bones/*immunology
MH  - Cryopreservation
MH  - HLA Antigens/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Plastic Surgery Procedures
MH  - Transplantation, Homologous
OTO - NOTNLM
OT  - Allografts
OT  - Bone graft
OT  - Dental implant
OT  - HLA
OT  - Immunology
OT  - Transplant
EDAT- 2016/10/27 06:00
MHDA- 2017/04/08 06:00
CRDT- 2016/10/27 06:00
PHST- 2016/08/03 00:00 [received]
PHST- 2016/10/21 00:00 [accepted]
PHST- 2016/10/27 06:00 [pubmed]
PHST- 2017/04/08 06:00 [medline]
PHST- 2016/10/27 06:00 [entrez]
AID - 10.1007/s10561-016-9594-1 [pii]
AID - 10.1007/s10561-016-9594-1 [doi]
PST - ppublish
SO  - Cell Tissue Bank. 2017 Mar;18(1):75-81. doi: 10.1007/s10561-016-9594-1. Epub 2016 
      Oct 25.