PMID- 27784763
OWN - NLM
STAT- MEDLINE
DCOM- 20170612
LR  - 20220129
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 473
IP  - 24
DP  - 2016 Dec 15
TI  - Differential effects of pannexins on noise-induced hearing loss.
PG  - 4665-4680
AB  - Hearing loss, including noise-induced hearing loss, is highly prevalent and 
      severely hinders an individual's quality of life, yet many of the mechanisms that 
      cause hearing loss are unknown. The pannexin (Panx) channel proteins, Panx1 and 
      Panx3, are regionally expressed in many cell types along the auditory pathway, 
      and mice lacking Panx1 in specific cells of the inner ear exhibit hearing loss, 
      suggesting a vital role for Panxs in hearing. We proposed that Panx1 and/or Panx3 
      null mice would exhibit severe hearing loss and increased susceptibility to 
      noise-induced hearing loss. Using the auditory brainstem response, we 
      surprisingly found that Panx1(-/-) and Panx3(-/-) mice did not harbor hearing or 
      cochlear nerve deficits. Furthermore, while Panx1(-/-) mice displayed no 
      protection against loud noise-induced hearing loss, Panx3(-/-) mice exhibited 
      enhanced 16- and 24-kHz hearing recovery 7 days after a loud noise exposure (NE; 
      12 kHz tone, 115 dB sound pressure level, 1 h). Interestingly, Cx26, Cx30, Cx43, 
      and Panx2 were up-regulated in Panx3(-/-) mice compared with wild-type and/or 
      Panx1(-/-) mice, and assessment of the auditory tract revealed morphological 
      changes in the middle ear bones of Panx3(-/-) mice. It is unclear if these 
      changes alone are sufficient to provide protection against loud noise-induced 
      hearing loss. Contrary to what we expected, these data suggest that Panx1 and 
      Panx3 are not essential for baseline hearing in mice tested, but the therapeutic 
      targeting of Panx3 may prove protective against mid-high-frequency hearing loss 
      caused by loud NE.
CI  - (c) 2016 The Author(s); published by Portland Press Limited on behalf of the 
      Biochemical Society.
FAU - Abitbol, Julia M
AU  - Abitbol JM
AD  - Department of Anatomy and Cell Biology, The University of Western Ontario, 
      London, Ontario N6A 5C1, Canada.
FAU - Kelly, John J
AU  - Kelly JJ
AD  - Department of Anatomy and Cell Biology, The University of Western Ontario, 
      London, Ontario N6A 5C1, Canada.
FAU - Barr, Kevin
AU  - Barr K
AD  - Department of Anatomy and Cell Biology, The University of Western Ontario, 
      London, Ontario N6A 5C1, Canada.
AD  - Department of Physiology and Pharmacology, The University of Western Ontario, 
      London, Ontario, Canada.
FAU - Schormans, Ashley L
AU  - Schormans AL
AD  - Department of Anatomy and Cell Biology, The University of Western Ontario, 
      London, Ontario N6A 5C1, Canada.
FAU - Laird, Dale W
AU  - Laird DW
AD  - Department of Anatomy and Cell Biology, The University of Western Ontario, 
      London, Ontario N6A 5C1, Canada.
AD  - Department of Physiology and Pharmacology, The University of Western Ontario, 
      London, Ontario, Canada.
FAU - Allman, Brian L
AU  - Allman BL
AD  - Department of Anatomy and Cell Biology, The University of Western Ontario, 
      London, Ontario N6A 5C1, Canada.
LA  - eng
GR  - F40/ACT_/RNID/United Kingdom
GR  - 130530/CIHR/Canada
GR  - 148584/CIHR/Canada
GR  - 137098/CIHR/Canada
PT  - Journal Article
DEP - 20161026
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Connexin 30)
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (Gjb2 protein, mouse)
RN  - 0 (Gjb6 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Panx1 protein, mouse)
RN  - 0 (Panx2 protein, mouse)
RN  - 0 (pannexin 3 protein, mouse)
RN  - 127120-53-0 (Connexin 26)
SB  - IM
MH  - Animals
MH  - Cochlea/metabolism/physiology
MH  - Connexin 26
MH  - Connexin 30
MH  - Connexin 43/metabolism
MH  - Connexins/genetics/*metabolism
MH  - Evoked Potentials, Auditory, Brain Stem/genetics/physiology
MH  - Hearing Loss, Noise-Induced/genetics/*metabolism
MH  - Immunoblotting
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - auditory brainstem response
OT  - hearing
OT  - mouse
OT  - noise-induced hearing loss
OT  - pannexins
EDAT- 2016/10/28 06:00
MHDA- 2017/06/13 06:00
CRDT- 2016/10/28 06:00
PHST- 2016/07/11 00:00 [received]
PHST- 2016/10/14 00:00 [revised]
PHST- 2016/10/24 00:00 [accepted]
PHST- 2016/10/28 06:00 [pubmed]
PHST- 2017/06/13 06:00 [medline]
PHST- 2016/10/28 06:00 [entrez]
AID - BCJ20160668 [pii]
AID - 10.1042/BCJ20160668 [doi]
PST - ppublish
SO  - Biochem J. 2016 Dec 15;473(24):4665-4680. doi: 10.1042/BCJ20160668. Epub 2016 Oct 
      26.