PMID- 27788707
OWN - NLM
STAT- MEDLINE
DCOM- 20170927
LR  - 20220317
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 24
IP  - 8
DP  - 2016 Aug 20
TI  - [Effect of JAZF1 over-expression on high-fat diet-induced non-alcoholic fatty 
      liver disease].
PG  - 596-600
LID - 10.3760/cma.j.issn.1007-3418.2016.08.008 [doi]
AB  - Objective: To investigate the effects of juxtaposed with another zinc finger gene 
      1 (JAZF1) over-expression on the levels of pro-inflammatory cytokines in high-fat 
      diet (HFD)-induced mouse fatty liver and its associated mechanisms. Methods: 
      Twenty male C57BL/6J (3 weeks old) and 10 male JAZF1 transgenic (JAZF1-Tg) mice 
      were randomly divided into three groups: wide-type with normal diet (NF group,n= 
      10), wide-type with high-fat diet (HF group,n= 10), and JAZF1-Tg with high-fat 
      diet (HJ group,n= 10). All mice were fed with the corresponding diet for 12 
      weeks, and their food consumption and body weight were measured periodically. 
      After 12 weeks, fasting blood glucose (FBG), insulin (INS), total cholesterol 
      (TC), triglyceride (TG), free fatty acids (FFA), and alanine aminotransferase 
      (ALT) in the blood and liver tissue from each group were measured. TG 
      concentration in liver tissue was determined using an enzymatic assay, and the 
      mRNA expression of tumor necrosis factor-alpha(TNF-alpha), monocyte chemoattractant 
      protein-1 (MCP-1), and interleukin-8 (IL-8) in the liver was measured by RT-PCR. 
      In addition, the expression of p-JNK/JNK, p-p-38 MAPK/p-38 MAPK, p-ERK/ERK, IkappaBalpha, 
      andbeta-actin (reference) in the liver was determined using Western blot.. Results: 
      (1) Body weight, FBG, INS, TC, and ALT were significantly reduced in the HJ group 
      compared with those of the HF group (31.19+/-0.81 vs 36.07+/-1.43, 6.94+/-0.32 vs 
      8.14+/-0.36, 31.09+/-2.12 vs 45.21+/-3.34, 3.05+/-0.07 vs 3.81+/-0.08, 54.75+/-4.92 vs 
      68.09+/-5.15, respectively;P< 0.05). There were no significant differences in TG 
      and FFA between the HJ and HF groups (0.72+/-0.05 vs 0.81+/-0.03, 0.81+/-0.4 vs 
      0.87+/-0.03; bothP> 0.05). (2) There was no significant difference in liver TG 
      concentration between the HJ and HF groups (35.49+/-3.17 vs 38.26+/-3.59,P> 0.05). 
      (3) Compared with the HF group, the HJ group had significantly reduced mRNA 
      expression of TNF-alpha, MCP-1, and IL-8 (2.54TNF-alphavs 8.64+/-0.73, 1.19+/-0.73,vs 
      3.93+/-0.18, 5.09+/-0.48 vs 9.09+/-0.89; allP< 0.01), significantly reduced protein 
      expression of p-JNK and p-p-38 MAPK (0.92+/-0.06 vs 1.51+/-0.01, 1.07+/-0.04 vs 
      1.45+/-0.04; bothP< 0.01), and significantly increased protein expression of 
      IkappaBalpha(0.99+/-0.06 vs 0.79+/-0.05,P< 0.01) in liver tissue. However, no significant 
      difference was observed in the p-ERK level between the HJ and HF groups (P> 
      0.05). Conclusion: Upregulation of JAZF1 expression can significantly inhibit the 
      expression of TNF-alpha, MCP-1, and IL-8 in the liver of mice on HFD. This 
      attenuation may be closely associated with the reduced activation of the JNK, 
      p-38 MAPK, and NF-kappaB pathways.
FAU - Hu, W J
AU  - Hu WJ
AD  - Chongqing Treatment and Prevention Center for Occupational Diseases, Chongqing 
      400060, China.
FAU - Shen, W W
AU  - Shen WW
AD  - Chongqing Treatment and Prevention Center for Occupational Diseases, Chongqing 
      400060, China.
FAU - Li, X J
AU  - Li XJ
AD  - Chongqing Treatment and Prevention Center for Occupational Diseases, Chongqing 
      400060, China.
FAU - Yao, J
AU  - Yao J
AD  - Chongqing Treatment and Prevention Center for Occupational Diseases, Chongqing 
      400060, China.
FAU - Jia, Y J
AU  - Jia YJ
AD  - Department of Laboratory Medicine, M.O.E. Key Laboratory of Laboratory Medicine 
      Diagnostics, Chongqing Medical University, 400016 Chongqing, China.
FAU - Fan, X Y
AU  - Fan XY
AD  - Chongqing Treatment and Prevention Center for Occupational Diseases, Chongqing 
      400060, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Carrier Proteins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (Cytokines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Insulin)
RN  - 0 (Interleukin-8)
RN  - 0 (JAZF1 protein, mouse)
RN  - 0 (NF-kappa B)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase
MH  - Animals
MH  - Body Weight
MH  - Carrier Proteins/*genetics/metabolism
MH  - Chemokine CCL2
MH  - Cholesterol
MH  - Co-Repressor Proteins
MH  - Cytokines
MH  - DNA-Binding Proteins
MH  - *Diet, High-Fat
MH  - Insulin
MH  - Insulin Resistance
MH  - Interleukin-8
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B
MH  - Non-alcoholic Fatty Liver Disease/*metabolism
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Tumor Necrosis Factor-alpha
EDAT- 2016/10/30 06:00
MHDA- 2017/09/28 06:00
CRDT- 2016/10/30 06:00
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2016/10/30 06:00 [entrez]
AID - 10.3760/cma.j.issn.1007-3418.2016.08.008 [doi]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2016 Aug 20;24(8):596-600. doi: 
      10.3760/cma.j.issn.1007-3418.2016.08.008.