PMID- 27789903
OWN - NLM
STAT- MEDLINE
DCOM- 20170505
LR  - 20190606
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 22
DP  - 2016 Oct 28
TI  - Dynamic Perfusion Culture of Human Outgrowth Endothelial Progenitor Cells on 
      Demineralized Bone Matrix In Vitro.
PG  - 4037-4045
AB  - BACKGROUND The aim of this study was to investigate the proliferation, 
      differentiation, and tube formation of human outgrowth endothelial progenitor 
      cells (OECs) cultured with porous demineralized bone matrix (DBM) under a dynamic 
      perfusion system in vitro. MATERIAL AND METHODS OECs were isolated, expanded, 
      characterized, eGFP-transfected and seeded on DBM scaffold and cultured under 
      static or dynamic perfusion conditions, and continuously observed under 
      fluorescence microscope. DBM scaffolds were harvested on day six for RT-PCR and 
      western blot assay to analyze the mRNA and protein expression level of CD34, 
      VE-cadherin, and VEGF. Scanning electron microscope (SEM) was used to observe the 
      tube formation of OECs seeded on DBM scaffolds. RESULTS The results showed the 
      cell density of OECs on DBM was higher when exposed to shear stress generated by 
      a dynamic perfusion system. Shear stress also markedly increased the expression 
      level of VE-cadherin and VEGF and decreased the expression of CD34, at both mRNA 
      and protein levels. SEM showed that the shear-stressed OECs formed tube-like 
      structures inside the pores of DBM scaffolds. CONCLUSIONS A dynamic perfusion 
      system can be used as an innovative method for the rapid vascularization in 
      tissue engineering, which can accelerate the proliferation and differentiation of 
      OECs and the vascularization of implanted scaffolds.
FAU - Cai, Di-Xin
AU  - Cai DX
AD  - Department of Orthopedics, Kunming Medical University, Kunming, China (mainland).
FAU - Quan, Yue
AU  - Quan Y
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen 
      University, Xiamen, Fujian, China (mainland).
FAU - He, Peng-Ju
AU  - He PJ
AD  - Department of Orthopedics, Kunming General Hospital of Chengdu Military Region, 
      Kunming, China (mainland).
FAU - Tan, Hong-Bo
AU  - Tan HB
AD  - Department of Orthopedics, Kunming General Hospital of Chengdu Military Region, 
      Kunming, China (mainland).
FAU - Xu, Yong-Qing
AU  - Xu YQ
AD  - Department of Orthopedics, Kunming General Hospital of Chengdu Military Region, 
      Kunming, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20161028
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Biocompatible Materials)
SB  - IM
MH  - Biocompatible Materials
MH  - Bone Matrix/cytology
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Endothelial Progenitor Cells/*cytology/transplantation
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology
MH  - Neovascularization, Physiologic/physiology
MH  - Osteogenesis/physiology
MH  - Perfusion
MH  - Stress, Mechanical
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
PMC - PMC5098931
EDAT- 2016/10/30 06:00
MHDA- 2017/05/06 06:00
PMCR- 2016/10/28
CRDT- 2016/10/30 06:00
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2017/05/06 06:00 [medline]
PHST- 2016/10/30 06:00 [entrez]
PHST- 2016/10/28 00:00 [pmc-release]
AID - 897884 [pii]
AID - 10.12659/msm.897884 [doi]
PST - epublish
SO  - Med Sci Monit. 2016 Oct 28;22:4037-4045. doi: 10.12659/msm.897884.