PMID- 27790153
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 7
DP  - 2016
TI  - Nerve Growth Factor, Brain-Derived Neurotrophic Factor and Osteocalcin Gene 
      Relationship in Energy Regulation, Bone Homeostasis and Reproductive Organs 
      Analyzed by mRNA Quantitative Evaluation and Linear Correlation Analysis.
PG  - 456
LID - 456
AB  - Nerve Growth Factor (NGF)/Brain-derived Neurotrophic Factor (BDNF) and 
      osteocalcin share common effects regulating energy, bone mass, reproduction and 
      neuronal functions. To investigate on the gene-relationship between NGF, BDNF, 
      and Osteocalcin we compared by RT-PCR the transcript levels of Ngf, Bdnf and 
      Osteocalcin as well as of their receptors p75NTR/NTRK1, NTRK2, and Gprc6a in 
      brain, bone, white/brown adipose tissue (WAT/BAT) and reproductive organs of 3 
      months old female and male mice. Brain and bone were used as positive controls 
      for NGF/BDNF and Osteocalcin respectively. The role of oxitocin(Oxt) and its 
      receptor(Oxtr) was also investigated. Ngf expression shows an opposite trend 
      compared to Bdnf. Ngf /p75NTR expression is 50% higher in BAT than brain, in both 
      genders, but lower in bone. In contrast, Bdnf expression in bone is higher than 
      in brain, but low in BAT/WAT. We found Osteocalcin gene expressed in brain in 
      both genders, but Gprc6a expression is low in brain and BAT/WAT. As expected, 
      Gprc6a gene is expressed in bone. Oxt gene was markedly expressed in brain, Oxtr 
      in the ovaries and in fat and bone in both genders. Ngf is highly expressed in 
      reproductive tissues and p75NTR mRNA levels are respectively 300, 100, and 50% 
      higher in testis/ovaries/uterus than in brain. In contrast, BDNF genes are not 
      expressed in reproductive tissues. As expected, Gprc6a is expressed in testis but 
      not in the ovaries/uterus. A significant correlation was found between the 
      expression levels of the gene ligands and their receptors in brain, BAT and 
      testis suggesting a common pathway of different genes in these tissues in either 
      male and female. Changes in the expression levels of osteocalcin, Ngf, or Bdnf 
      genes may mutually affect the expression levels of the others. Moreover, it may 
      be possible that different ligands may operate through different receptor 
      subtypes. Oxt and Oxtr failed to show significant correlation. The up-regulation 
      of Ngf /p75NTR in BAT is consistent with NGF as an energy regulator and with BDNF 
      regulating bone.
FAU - Camerino, Claudia
AU  - Camerino C
AD  - Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 
      of BariBari, Italy; Department of Molecular and Cellular Physiology, University 
      of CincinnatiCincinnati, OH, USA.
FAU - Conte, Elena
AU  - Conte E
AD  - Department of Pharmacy - Drug Sciences, University of Bari Bari, Italy.
FAU - Cannone, Maria
AU  - Cannone M
AD  - Department of Pharmacy - Drug Sciences, University of Bari Bari, Italy.
FAU - Caloiero, Roberta
AU  - Caloiero R
AD  - Department of Pharmacy - Drug Sciences, University of Bari Bari, Italy.
FAU - Fonzino, Adriano
AU  - Fonzino A
AD  - Department of Pharmacy - Drug Sciences, University of Bari Bari, Italy.
FAU - Tricarico, Domenico
AU  - Tricarico D
AD  - Department of Pharmacy - Drug Sciences, University of Bari Bari, Italy.
LA  - eng
PT  - Journal Article
DEP - 20161013
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC5061807
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - energy regulation
OT  - gene expression
OT  - nerve growth factor
OT  - osteocalcin
EDAT- 2016/10/30 06:00
MHDA- 2016/10/30 06:01
PMCR- 2016/10/13
CRDT- 2016/10/30 06:00
PHST- 2016/08/11 00:00 [received]
PHST- 2016/09/22 00:00 [accepted]
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2016/10/30 06:01 [medline]
PHST- 2016/10/30 06:00 [entrez]
PHST- 2016/10/13 00:00 [pmc-release]
AID - 10.3389/fphys.2016.00456 [doi]
PST - epublish
SO  - Front Physiol. 2016 Oct 13;7:456. doi: 10.3389/fphys.2016.00456. eCollection 
      2016.