PMID- 27793764
OWN - NLM
STAT- MEDLINE
DCOM- 20170202
LR  - 20220310
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 264
DP  - 2016 Dec 15
TI  - Cyanidin-3-O-glucoside inhibits NF-kB signalling in intestinal epithelial cells 
      exposed to TNF-alpha and exerts protective effects via Nrf2 pathway activation.
PG  - 51-58
LID - S0378-4274(16)33288-X [pii]
LID - 10.1016/j.toxlet.2016.10.014 [doi]
AB  - Chronic intestinal inflammatory disorders, such as Inflammatory Bowel Diseases 
      (IBDs), are characterized by excessive release of proinflammatory mediators, 
      intestinal barrier dysfunction and excessive activation of NF-kB cascade. 
      Previous studies shown that TNF-alpha plays a central role in intestinal inflammation 
      of IBDs and supported beneficial effects of flavonoids against chronic 
      inflammatory diseases. In this study, we employed an in vitro model of acute 
      intestinal inflammation using intestinal Caco-2 cells exposed to TNF-alpha. The 
      protective effects of cyanidin-3-glucoside (C3G), an anthocyanin widely 
      distributed in mediterranean diet, were then evaluated. Caco-2 cells exposure to 
      TNF-alpha activated NF-kB proinflammatory pathway and induced IL6 and COX-2 
      expression. Cells pretreatment for 24h with C3G (20-40muM) prevented TNF-alpha-induced 
      changes, and improved intracellular redox status. Our results demonstrated that 
      C3G, also without any kind of stimulus, increased the translocation of the 
      transcription factor Nrf2 into the nucleus so activating antioxidant and 
      detoxifying genes. In conclusion, C3G exhibited protective effects through the 
      inhibition of NF-kB signalling in Caco-2 cells and these beneficial effects 
      appear to be due to its ability to activate cellular protective responses 
      modulated by Nrf2. These data suggest that anthocyanins could contribute, as 
      complementary or preventive approaches, to the management of chronic inflammatory 
      diseases.
CI  - Copyright A(c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Ferrari, Daniela
AU  - Ferrari D
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Italy. Electronic address: dani841984@libero.it.
FAU - Speciale, Antonio
AU  - Speciale A
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Italy. Electronic address: specialea@unime.it.
FAU - Cristani, Mariateresa
AU  - Cristani M
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Italy. Electronic address: mcristani@unime.it.
FAU - Fratantonio, Deborah
AU  - Fratantonio D
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Italy. Electronic address: dfratantonio2@unl.edu.
FAU - Molonia, Maria Sofia
AU  - Molonia MS
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Italy. Electronic address: mmolonia@unime.it.
FAU - Ranaldi, Giulia
AU  - Ranaldi G
AD  - Council for Agricultural Research and Economics, Food and Nutrition Research 
      Centre, (CREA - NUT), Rome, Italy. Electronic address: 
      giulia.ranaldi@crea.gov.it.
FAU - Saija, Antonella
AU  - Saija A
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Italy. Electronic address: asaija@unime.it.
FAU - Cimino, Francesco
AU  - Cimino F
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Italy. Electronic address: fcimino@unime.it.
LA  - eng
PT  - Journal Article
DEP - 20161025
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Anthocyanins)
RN  - 0 (Antioxidants)
RN  - 0 (Glucosides)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Prostaglandins)
RN  - 0 (Thromboxanes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (cyanidin-3-O-beta-glucopyranoside)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Anthocyanins/*pharmacology
MH  - Antioxidants/metabolism
MH  - Caco-2 Cells
MH  - Cyclooxygenase 2/metabolism
MH  - Epithelial Cells/*drug effects
MH  - Glucosides/*pharmacology
MH  - Humans
MH  - Intestinal Mucosa/cytology/drug effects/metabolism
MH  - NF-E2-Related Factor 2/*agonists
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Prostaglandins/metabolism
MH  - Signal Transduction/drug effects
MH  - Thromboxanes/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
OTO - NOTNLM
OT  - 12303203)
OT  - Anthocyanin
OT  - Caco-2 cells
OT  - Cyanidin
OT  - Cyanidin 3-O-glucoside (PubChem CID
OT  - Inflammatory bowel diseases
OT  - NF-kB
OT  - Nrf2
OT  - Wedelolactone (PubChem CID: 5281813)
EDAT- 2016/10/30 06:00
MHDA- 2017/02/06 06:00
CRDT- 2016/10/30 06:00
PHST- 2016/09/07 00:00 [received]
PHST- 2016/10/21 00:00 [revised]
PHST- 2016/10/24 00:00 [accepted]
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
PHST- 2016/10/30 06:00 [entrez]
AID - S0378-4274(16)33288-X [pii]
AID - 10.1016/j.toxlet.2016.10.014 [doi]
PST - ppublish
SO  - Toxicol Lett. 2016 Dec 15;264:51-58. doi: 10.1016/j.toxlet.2016.10.014. Epub 2016 
      Oct 25.