PMID- 27794430
OWN - NLM
STAT- MEDLINE
DCOM- 20170516
LR  - 20171218
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 349
IP  - 2
DP  - 2016 Dec 10
TI  - Down-regulation of RBP-J mediated by microRNA-133a suppresses dendritic cells and 
      functions as a potential tumor suppressor in osteosarcoma.
PG  - 264-272
LID - S0014-4827(16)30344-5 [pii]
LID - 10.1016/j.yexcr.2016.10.019 [doi]
AB  - BACKGROUND AND OBJECTIVE: In recent years, immunotherapy for the treatment of 
      tumors have been established. Dendritic cells (DCs) are extremely efficient and 
      professional antigen presenting cells (APCs), which are an important target for 
      immune therapeutic interventions in cancer. In present study, we investigated 
      whether RBP-J signaling regulated by miR-133a was involved in the DCs mediated 
      tumor suppressor in osteosarcoma. METHODS: DCs were isolated from 30 osteosarcoma 
      patients and 30 healthy subjects. Mouse macrophage-like cell line RAW264.7 were 
      cultured and osteosarcoma mouse model with injection of murine osteosarcoma cell 
      line S180 were established. RESULTS: In osteosarcoma patients, miR-133a 
      expression level of DCs was increased, and RBP-J expression in mRNA and protein 
      levels were decreased. MiR-133a inhibitor promoted maturation and activation of 
      DCs in osteosarcoma patients. In osteosarcoma mouse model, miR-133a mimic 
      suppressed the maturation and activation of spleen DCs, while miR-133a inhibitor 
      promoted them. Overexpression of miR-133a decreased therapeutic effect of DCs on 
      osteosarcoma mice. In RAW264.7 cells, miR-133a was observed to target RBP-J and 
      regulate its expression. MiR-133a mimic inhibited the maturation of DCs in cells 
      exposed to LPS, the effect of which was reversed by overexpression of RBP-J. 
      CONCLUSION: RBP-J mediated by miR-133a probably contributed to the regulation of 
      DCs maturation and activation in osteosarcoma, which functioned as a therapeutic 
      target for the immunotherapy in cancers.
CI  - Copyright (c) 2016. Published by Elsevier Inc.
FAU - Gao, Xuren
AU  - Gao X
AD  - Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, PR China; Department of Orthopedics, The 
      Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, PR 
      China.
FAU - Han, Dong
AU  - Han D
AD  - Central laboratory, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 
      Jiangsu 221002, PR China.
FAU - Fan, Weimin
AU  - Fan W
AD  - Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210000, PR China. Electronic address: 
      weiminfxz@163.com.
LA  - eng
PT  - Journal Article
DEP - 20161026
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Immunoglobulin J Recombination Signal Sequence-Binding Protein)
RN  - 0 (MIRN133 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn133 microRNA, mouse)
RN  - 0 (RBPJ protein, human)
RN  - 0 (Rbpj protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Neoplasms/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Dendritic Cells/*cytology
MH  - Down-Regulation
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Tumor Suppressor/physiology
MH  - Humans
MH  - Immunoglobulin J Recombination Signal Sequence-Binding Protein/*genetics
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Osteosarcoma/*genetics
OTO - NOTNLM
OT  - Dendritic cells
OT  - Immunotherapy
OT  - Osteosarcoma
OT  - RBP-J
OT  - miR-133a
EDAT- 2016/10/31 06:00
MHDA- 2017/05/17 06:00
CRDT- 2016/11/08 06:00
PHST- 2016/08/03 00:00 [received]
PHST- 2016/10/14 00:00 [revised]
PHST- 2016/10/20 00:00 [accepted]
PHST- 2016/10/31 06:00 [pubmed]
PHST- 2017/05/17 06:00 [medline]
PHST- 2016/11/08 06:00 [entrez]
AID - S0014-4827(16)30344-5 [pii]
AID - 10.1016/j.yexcr.2016.10.019 [doi]
PST - ppublish
SO  - Exp Cell Res. 2016 Dec 10;349(2):264-272. doi: 10.1016/j.yexcr.2016.10.019. Epub 
      2016 Oct 26.