PMID- 27795806
OWN - NLM
STAT- MEDLINE
DCOM- 20170714
LR  - 20181113
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2016
DP  - 2016
TI  - C1q/TNF-Related Protein 9 Protects Diabetic Rat Heart against Ischemia 
      Reperfusion Injury: Role of Endoplasmic Reticulum Stress.
PG  - 1902025
LID - 1902025
AB  - As a newly identified adiponectin paralog, C1q/TNF-related protein 9 (CTRP9) 
      reduces myocardial ischemia reperfusion (IR) injury through partially understood 
      mechanisms. In the present study, we sought to identify the role of endoplasmic 
      reticulum stress (ERS) in CTRP9 induced cardioprotection in diabetic heart. 
      Isolated hearts from high-fat-diet (HFD) induced type 2 diabetic Sprague-Dawley 
      rats were subjected to ex vivo IR protocol via a Langendorff apparatus at the 
      presence of globular CTRP9. CTRP9 significantly improved post-IR heart function 
      and reduced cardiac infarction, cardiomyocytes apoptosis, Caspase-3, Caspase-9, 
      Caspase-12, TNF-alpha expression, and lactate dehydrogenase activity. The 
      cardioprotective effect of CTRP9 was associated with reduced ERS and increased 
      expression of disulfide-bond A oxidoreductase-like protein (DsbA-L) in diabetic 
      heart. CTRP9 reduced ERS in thapsigargin (TG) treated cardiomyocytes and 
      protected endoplasmic reticulum (ER) stressed H9c2 cells against simulated 
      ischemia reperfusion (SIR) injury, concurrent with increased expression of 
      DsbA-L. Knockdown of DsbA-L increased ERS and attenuated CTRP9 induced protection 
      against SIR injury in H9c2 cells. Our findings demonstrated for the first time 
      that CTRP9 exerts cardioprotection by reducing ERS in diabetic heart through 
      increasing DsbA-L.
FAU - Bai, Sanxing
AU  - Bai S
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Cheng, Liang
AU  - Cheng L
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Biomedical Engineering, The Fourth Military Medical University, 169 
      Changle West Road, Xi'an 710032, China.
FAU - Fan, Chongxi
AU  - Fan C
AD  - Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical 
      University, 1 Xinsi Road, Xi'an 710038, China.
FAU - Zhao, Dajun
AU  - Zhao D
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Qin, Zhigang
AU  - Qin Z
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Feng, Xiao
AU  - Feng X
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Zhao, Lin
AU  - Zhao L
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Ma, Jipeng
AU  - Ma J
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Wang, Xiaowu
AU  - Wang X
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Yang, Jian
AU  - Yang J
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Xu, Xuezeng
AU  - Xu X
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Yi, Dinghua
AU  - Yi D
AUID- ORCID: 0000-0001-8194-8837
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
FAU - Yi, Wei
AU  - Yi W
AUID- ORCID: 0000-0001-6478-6346
AD  - Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military 
      Medical University, 127 Changle West Road, Xi'an 710032, China.
LA  - eng
GR  - K99 AA020514/AA/NIAAA NIH HHS/United States
GR  - R00 AA020514/AA/NIAAA NIH HHS/United States
GR  - R01 AA020919/AA/NIAAA NIH HHS/United States
PT  - Journal Article
DEP - 20161004
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Adiponectin)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Adiponectin/*pharmacology
MH  - Animals
MH  - Cell Line
MH  - Diabetes Mellitus, Experimental/metabolism
MH  - Endoplasmic Reticulum Stress/*drug effects/physiology/radiation effects
MH  - Gene Knockdown Techniques
MH  - Glutathione Transferase/genetics/metabolism
MH  - Heart/drug effects/radiation effects
MH  - Isolated Heart Preparation
MH  - Male
MH  - Myocardial Reperfusion Injury/metabolism/*prevention & control
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
PMC - PMC5067328
EDAT- 2016/11/01 06:00
MHDA- 2017/07/15 06:00
PMCR- 2016/10/04
CRDT- 2016/11/01 06:00
PHST- 2016/03/15 00:00 [received]
PHST- 2016/08/06 00:00 [revised]
PHST- 2016/08/28 00:00 [accepted]
PHST- 2016/11/01 06:00 [pubmed]
PHST- 2017/07/15 06:00 [medline]
PHST- 2016/11/01 06:00 [entrez]
PHST- 2016/10/04 00:00 [pmc-release]
AID - 10.1155/2016/1902025 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2016;2016:1902025. doi: 10.1155/2016/1902025. Epub 2016 Oct 
      4.