PMID- 27800078
OWN - NLM
STAT- MEDLINE
DCOM- 20170210
LR  - 20181113
IS  - 1937-8688 (Electronic)
VI  - 24
DP  - 2016
TI  - [Molecular tests in diagnosis of Cytomegalovirus (CMV), human herpesvirus 6 
      (HHV-6) and Epstein-Barr virus (EBV) using real-time PCR in HIV positive and 
      HIV-negative pregnant women in Ouagadougou, Burkina Faso].
PG  - 223
LID - 223
AB  - INTRODUCTION: Herpesvirus EBV, CMV and HHV-6 are viruses that evolve based on 
      pandemic modeling and are responsible for congenital infections causing severe 
      sequelae in infants. This study aims to determine the prevalence of CMV, EBV and 
      HHV-6 among HIV (+) and HIV (-) pregnant women in Ouagadougou. METHODS: In this 
      study 200 blood plasma samples taken from pregnant women, of whom 100 with HIV(+) 
      and 100 with HIV(-), were analyzed using multiplex real-time PCR which detected 
      three infections (EBV, CMV and HHV-6). RESULTS: Out of the 200 samples tested, 
      18(9.0%) were positive for at least one of the three viruses, 12(6.0%) were 
      positive for EBV, 13(6.5%) were positive for CMV and 12(6.0%) were positive for 
      HHV-6. Among the 18 cases with infections, 10 cases (55.6%) had co-infections of 
      whom 90.0% (9/10) with multiple EBV/CMV/HHV6 infection and 10.0% with EBV/HHV6 
      co-infection. HHVs infection rate was higher among HIV (-) pregnant women than 
      among HIV (+) pregnant women (12.0% versus 6.0%). Among HIV (+) pregnant women, 
      PCR showed 7.1% (6/85) of HHVs infection in patients who were not treated with 
      ARV against 0% in those treated with ARVs. CONCLUSION: Herpes virus infections 
      are a common condition in pregnant women in Burkina Faso. They may represent a 
      real threat to pregnant women because of complications and risks of infection in 
      infants.
FAU - Ouedraogo, Alice Rogomenoma
AU  - Ouedraogo AR
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Kabre, Madeleine
AU  - Kabre M
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Bisseye, Cyrille
AU  - Bisseye C
AD  - Laboratoire de Biologie Moleculaire et Cellulaire (LABMC), Universite des 
      Sciences et Techniques de Masuku (USTM), Franceville, Gabon.
FAU - Zohoncon, Theodora Mahoukede
AU  - Zohoncon TM
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Asshi, Maleki
AU  - Asshi M
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Soubeiga, Serge Theophile
AU  - Soubeiga ST
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Diarra, Birama
AU  - Diarra B
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Traore, Lassina
AU  - Traore L
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Djigma, Florencia Wendkuuni
AU  - Djigma FW
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Ouermi, Djeneba
AU  - Ouermi D
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Pietra, Virginio
AU  - Pietra V
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
FAU - Barro, Nicolas
AU  - Barro N
AD  - Laboratoire de Biologie Moleculaire, d'Epidemiologie et de Surveillance des 
      Bacteries et virus Transmissibles par les Aliments (LaBESTA), UFR/SVT, Universite 
      de Ouagadougou.
FAU - Simpore, Jacques
AU  - Simpore J
AD  - Centre de Recherche Biomoleculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, 
      Universite de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
LA  - fre
PT  - Journal Article
TT  - Diagnostic moleculaire du Cytomegalovirus (CMV), de l'herpes virus humain de type 
      6 (HHV6) et d'Epstein-Barr virus (EBV) par PCR en temps reel chez les femmes 
      enceintes VIH seropositives et seronegatives a Ouagadougou, Burkina Faso.
DEP - 20160712
PL  - Uganda
TA  - Pan Afr Med J
JT  - The Pan African medical journal
JID - 101517926
SB  - IM
MH  - Adult
MH  - Burkina Faso/epidemiology
MH  - Coinfection
MH  - Cytomegalovirus Infections/complications/*diagnosis/epidemiology
MH  - Epstein-Barr Virus Infections/complications/*diagnosis/epidemiology
MH  - Female
MH  - HIV Infections/epidemiology
MH  - Herpesviridae Infections/complications/*diagnosis/epidemiology
MH  - Humans
MH  - Molecular Diagnostic Techniques/methods
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/*diagnosis/epidemiology/virology
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Young Adult
PMC - PMC5075482
OTO - NOTNLM
OT  - Burkina Faso
OT  - HHVs
OT  - HIV
OT  - multiplex real time PCR
OT  - pregnant woman
COIS- Conflits d'interets Les auteurs ne declarent aucun conflit d'interet.
EDAT- 2016/11/02 06:00
MHDA- 2017/02/12 06:00
PMCR- 2016/07/12
CRDT- 2016/11/02 06:00
PHST- 2016/03/22 00:00 [received]
PHST- 2016/05/23 00:00 [accepted]
PHST- 2016/11/02 06:00 [pubmed]
PHST- 2017/02/12 06:00 [medline]
PHST- 2016/11/02 06:00 [entrez]
PHST- 2016/07/12 00:00 [pmc-release]
AID - PAMJ-24-223 [pii]
AID - 10.11604/pamj.2016.24.223.9406 [doi]
PST - epublish
SO  - Pan Afr Med J. 2016 Jul 12;24:223. doi: 10.11604/pamj.2016.24.223.9406. 
      eCollection 2016.