PMID- 27801804
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 4
IP  - 4
DP  - 2016 Oct 27
TI  - Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated 
      Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells.
LID - 40
AB  - Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic 
      diseases, and the degranulation of mast cells is important in the pathogenesis of 
      these diseases. A disturbance of the intestinal microflora, especially of 
      endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated 
      allergic diseases. Additional knowledge regarding the interaction of human 
      intestinal Lactobacilli with mast cells is still necessary. Twenty-three strains 
      of Lactobacilli, including commercial and reference strains and strains from the 
      human intestine, were tested for their ability to regulate degranulation of cells 
      from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a 
      beta-hexosaminidase release assay. Each of the tested Lactobacilli 
      characteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, and 
      Lactobacillus GG showed the strongest inhibitory effect on the cells. 
      Furthermore, the bacteria isolated from the human intestine significantly 
      suppressed degranulation of RBL-2H3 cellsin comparison with the reference 
      strains. These results suggest that Lactobacilli, particularly those from the 
      human intestine, can affect the activation of mast cells in a strain-dependent 
      manner. Further study should be conducted to analyse the understanding mechanism.
FAU - Harata, Gaku
AU  - Harata G
AD  - Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 
      241-0023, Japan. G-harata@takanashi-milk.co.jp.
FAU - He, Fang
AU  - He F
AD  - Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 
      241-0023, Japan. ka-hou@takanashi-milk.co.jp.
FAU - Takahashi, Kyoko
AU  - Takahashi K
AD  - Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, 
      Nihon University, Fujisawa 252-8510, Japan. ktaka@brs.nihon-u.ac.jp.
FAU - Hosono, Akira
AU  - Hosono A
AD  - Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, 
      Nihon University, Fujisawa 252-8510, Japan. hosono@brs.nihon-u.ac.jp.
FAU - Miyazawa, Kenji
AU  - Miyazawa K
AD  - Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 
      241-0023, Japan. Ke-miyazawa@takakanashi-milk.co.jp.
FAU - Yoda, Kazutoyo
AU  - Yoda K
AD  - Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 
      241-0023, Japan. K-yoda@takanashi-milk.co.jp.
FAU - Hiramatsu, Masaru
AU  - Hiramatsu M
AD  - Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 
      241-0023, Japan. M-hiramatsu@takanashi-milk.co.jp.
FAU - Kaminogawa, Shuichi
AU  - Kaminogawa S
AD  - Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, 
      Nihon University, Fujisawa 252-8510, Japan. kamino@b-star.jp.
LA  - eng
PT  - Journal Article
DEP - 20161027
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC5192523
OTO - NOTNLM
OT  - IgE-mediated allergy
OT  - Lactobacilli
OT  - degranulation
OT  - mast cell
COIS- The authors declare no conflict of interest.
EDAT- 2016/11/02 06:00
MHDA- 2016/11/02 06:01
PMCR- 2016/10/27
CRDT- 2016/11/02 06:00
PHST- 2016/08/15 00:00 [received]
PHST- 2016/10/07 00:00 [revised]
PHST- 2016/10/24 00:00 [accepted]
PHST- 2016/11/02 06:00 [pubmed]
PHST- 2016/11/02 06:01 [medline]
PHST- 2016/11/02 06:00 [entrez]
PHST- 2016/10/27 00:00 [pmc-release]
AID - microorganisms4040040 [pii]
AID - microorganisms-04-00040 [pii]
AID - 10.3390/microorganisms4040040 [doi]
PST - epublish
SO  - Microorganisms. 2016 Oct 27;4(4):40. doi: 10.3390/microorganisms4040040.