PMID- 27803402
OWN - NLM
STAT- MEDLINE
DCOM- 20170607
LR  - 20181202
IS  - 1349-7235 (Electronic)
IS  - 0918-2918 (Print)
IS  - 0918-2918 (Linking)
VI  - 55
IP  - 21
DP  - 2016
TI  - Triple Gene Analysis Using Samples Obtained by Endobronchial Ultrasound-guided 
      Transbronchial Needle Aspiration.
PG  - 3105-3111
AB  - Objective A mutational analysis of tumor tissue samples is an important part of 
      advanced lung cancer treatment strategies. This study evaluated the efficacy of a 
      triple gene analysis using samples obtained via endobronchial ultrasound-guided 
      transbronchial needle aspiration (EBUS-TBNA). Methods Either metastatic lymph 
      nodes or primary lung mass samples obtained by EBUS-TBNA were collected between 
      May 2011 and May 2013. We consecutively analyzed epidermal growth factor receptor 
      (EGFR), V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), and 
      anaplastic lymphoma kinase (ALK) fusion genes using remnant tissue samples. 
      Results A total of 109 patients were diagnosed with non-small cell lung cancer 
      (NSCLC). Of these, 70% were adenocarcinoma, 27% squamous cell carcinoma with 
      NSCLC, and 3% were related to other types of lung cancer. EGFR mutations were 
      detected in 23 cases (21.1%), KRAS mutations in 13 cases (11.9%), and ALK fusion 
      genes in 5 cases (4.9%). The ALK fusion genes could not be analyzed in four cases 
      because of insufficient tissue samples remaining after routine histochemistry and 
      an EGFR/KRAS mutation analysis. We found that small biopsy samples from EBUS-TBNA 
      were adequate for performing a triple gene analysis in 97 patients (96%). ALK 
      fusion protein immunohistochemistry (IHC) was 100% consistent with fluorescence 
      in situ hybridization (FISH). Conclusion Small samples obtained by EBUS-TBNA were 
      found to be sufficient for performing a triple gene analysis following routine 
      histology and IHC. ALK IHC showed a very good concordance with FISH for detecting 
      ALK fusion genes.
FAU - Lee, Kyungjong
AU  - Lee K
AD  - Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung 
      Medical Center, Sungkyunkwan University School of Medicine, Korea.
FAU - Um, Sang-Won
AU  - Um SW
FAU - Jeong, Byeong-Ho
AU  - Jeong BH
FAU - Yang, Jung Wook
AU  - Yang JW
FAU - Choi, Yoon-La
AU  - Choi YL
FAU - Han, Joungho
AU  - Han J
FAU - Kim, Hojoong
AU  - Kim H
FAU - Kwon, O Jung
AU  - Kwon OJ
LA  - eng
PT  - Journal Article
DEP - 20161101
PL  - Japan
TA  - Intern Med
JT  - Internal medicine (Tokyo, Japan)
JID - 9204241
RN  - 0 (KRAS protein, human)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
SB  - IM
MH  - Adenocarcinoma/genetics/pathology
MH  - Adult
MH  - Aged
MH  - *Biopsy, Fine-Needle
MH  - Carcinoma, Non-Small-Cell Lung/genetics/pathology
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - *DNA Mutational Analysis
MH  - ErbB Receptors/metabolism
MH  - Female
MH  - *Genetic Testing
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lung/*pathology
MH  - Lung Neoplasms/*genetics/*pathology
MH  - Lymph Nodes/*pathology
MH  - Lymphatic Metastasis/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Proto-Oncogene Proteins p21(ras)/metabolism
MH  - *Ultrasonography, Interventional
PMC - PMC5140857
EDAT- 2016/11/03 06:00
MHDA- 2017/06/08 06:00
PMCR- 2016/11/01
CRDT- 2016/11/03 06:00
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2017/06/08 06:00 [medline]
PHST- 2016/11/03 06:00 [entrez]
PHST- 2016/11/01 00:00 [pmc-release]
AID - 10.2169/internalmedicine.55.6794 [doi]
PST - ppublish
SO  - Intern Med. 2016;55(21):3105-3111. doi: 10.2169/internalmedicine.55.6794. Epub 
      2016 Nov 1.