PMID- 27803790
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 19
IP  - 9
DP  - 2016 Sep
TI  - Total flavonoid extract from Coreopsis tinctoria Nutt. protects rats against 
      myocardial ischemia/reperfusion injury.
PG  - 1016-1023
AB  - OBJECTIVES: This study aimed to evaluate the protective effects of total 
      flavonoid extract from Coreopsis tinctoria Nutt. (CTF) against myocardial 
      ischemia/reperfusion injury (MIRI) using an isolated Langendorff rat heart model. 
      MATERIALS AND METHODS: Left ventricular developed pressure (LVDP) and the maximum 
      rate of rise and fall of LV pressure (+/-dp/dtmax) were recorded. Cardiac injury 
      was assessed by analyzing lactate dehydrogenase (LDH) and creatine kinase (CK) 
      released in the coronary effluent. Superoxide dismutase (SOD), glutathione 
      peroxidase (GSH-Px), and malondialdehyde (MDA) levels were determined. Myocardial 
      inflammation was assessed by monitoring tumor necrosis factor-alpha (TNF-alpha), 
      C-reactive protein (CRP), interleukin-8 (IL-8), and interleukin-6 (IL-6) levels. 
      Myocardial infarct size was estimated. Cell morphology was assessed by 
      2,3,5-triphenyltetrazolium chloride and hematoxylin and eosin (HE) staining. 
      Cardiomyocyte apoptosis was determined by terminal deoxynucleotidyl transferase 
      dUTP nick-end labeling (TUNEL) staining. RESULTS: Pretreatment with CTF 
      significantly increased the heart rate and increased LVDP, as well as SOD and 
      GSH-Px levels. In addition, CTF pretreatment decreased the TUNEL-positive cell 
      ratio, infarct size, and levels of CK, LDH, MDA, TNF-alpha, CRP, IL-6, and IL-8. 
      CONCLUSION: These results suggest that CTF exerts cardio-protective effects 
      against MIRI via anti-oxidant, anti-inflammatory, and anti-apoptotic activities.
FAU - Zhang, Ya
AU  - Zhang Y
AD  - Ministry of Education, Pharmacy Shihezi University Xinjiang China.
FAU - Yuan, Changsheng
AU  - Yuan C
AD  - Ministry of Education, Pharmacy Shihezi University Xinjiang China.
FAU - Fang, He
AU  - Fang H
AD  - Ministry of Education, Pharmacy Shihezi University Xinjiang China.
FAU - Li, Jia
AU  - Li J
AD  - Ministry of Education, Pharmacy Shihezi University Xinjiang China.
FAU - Su, Shanshan
AU  - Su S
AD  - Ministry of Education, Pharmacy Shihezi University Xinjiang China.
FAU - Chen, Wen
AU  - Chen W
AD  - Pharmacy Shihezi University Xinjiang China.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC5080418
OTO - NOTNLM
OT  - Anti-apoptosis
OT  - Anti-inflammatory
OT  - Anti-oxidant
OT  - Cardio-protective
OT  - Coreopsis tinctoria Nutt
EDAT- 2016/11/03 06:00
MHDA- 2016/11/03 06:01
PMCR- 2016/09/01
CRDT- 2016/11/03 06:00
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2016/11/03 06:01 [medline]
PHST- 2016/11/03 06:00 [entrez]
PHST- 2016/09/01 00:00 [pmc-release]
AID - IJBMS-19-1016 [pii]
PST - ppublish
SO  - Iran J Basic Med Sci. 2016 Sep;19(9):1016-1023.