PMID- 27803898
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 3
DP  - 2016
TI  - Candidate Gene Expression in Bos indicus Ovarian Tissues: Prepubertal and 
      Postpubertal Heifers in Diestrus.
PG  - 94
LID - 94
AB  - Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of 
      transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and 
      insulin-like growth factor system act as local regulators of ovarian follicular 
      development. To elucidate if these factors as well as others candidate genes, 
      such as estrogen receptor 1 (ESR1), growth differentiation factor 9 (GDF9), 
      follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), 
      bone morphogenetic protein receptor, type 2 (BMPR2), type 1 insulin-like growth 
      factor receptor (IGFR1), and key steroidogenic enzymes cytochrome P450 aromatase 
      and 3-beta-hydroxysteroid dehydrogenase (CYP19A1 and HSD3B1) could modulate or 
      influence diestrus on the onset of puberty in Brahman heifers, their ovarian mRNA 
      expression was measured before and after puberty (luteal phase). Six postpubertal 
      (POST) heifers were euthanized on the luteal phase of their second cycle, 
      confirmed by corpus luteum observation, and six prepubertal (PRE) heifers were 
      euthanized in the same day. Quantitative real-time PCR analysis showed that the 
      expression of FSHR, BMP7, CYP19A1, IGF1, and IGFR1 mRNA was greater in PRE 
      heifers, when contrasted to POST heifers. The expression of LHR and HSD3B1 was 
      lower in PRE heifers. Differential expression of ovarian genes could be 
      associated with changes in follicular dynamics and different cell populations 
      that have emerged as consequence of puberty and the luteal phase. The emerging 
      hypothesis is that BMP7 and IGF1 are co-expressed and may modulate the expression 
      of FSHR, LHR and IGFR1, and CYP19A1. BMP7 could influence the downregulation of 
      LHR and upregulation of FSHR and CYP19A1, which mediates the follicular dynamics 
      in heifer ovaries. Upregulation of IGF1 expression prepuberty, compared to 
      postpuberty diestrus, correlates with increased levels FSHR and CYP19A1. Thus, 
      BMP7 and IGF1 may play synergic roles and were predicted to interact, from the 
      expression data (P = 0.07, r = 0.84). The role of these co-expressed genes in 
      puberty and heifers luteal phase merits further research.
FAU - Weller, Mayara Morena Del Cambre Amaral
AU  - Weller MM
AD  - Animal Science Department, Universidade Federal de Vicosa, Vicosa, Minas Gerais, 
      Brazil; Queensland Alliance for Agriculture and Food Innovation, Centre for 
      Animal Science, The University of Queensland, Brisbane, QLD, Australia.
FAU - Fortes, Marina Rufino S
AU  - Fortes MR
AD  - School of Chemistry and Molecular Biosciences, The University of Queensland , 
      Brisbane, QLD , Australia.
FAU - Porto-Neto, Laercio R
AU  - Porto-Neto LR
AD  - CSIRO Agriculture Flagship, Queensland Bioscience Precinct , Brisbane, QLD , 
      Australia.
FAU - Kelly, Matthew
AU  - Kelly M
AD  - Queensland Alliance for Agriculture and Food Innovation, Centre for Animal 
      Science, The University of Queensland , Brisbane, QLD , Australia.
FAU - Venus, Bronwyn
AU  - Venus B
AD  - Queensland Alliance for Agriculture and Food Innovation, Centre for Animal 
      Science, The University of Queensland , Brisbane, QLD , Australia.
FAU - Kidd, Lisa
AU  - Kidd L
AD  - School of Veterinary Sciences, The University of Queensland , Gatton, QLD , 
      Australia.
FAU - do Rego, Joao Paulo Arcelino
AU  - do Rego JP
AD  - Instituto Federal de Educacao, Ciencia e Tecnologia do Ceara , Fortaleza, Ceara , 
      Brazil.
FAU - Edwards, Sophia
AU  - Edwards S
AD  - School of Veterinary Sciences, The University of Queensland , Gatton, QLD , 
      Australia.
FAU - Boe-Hansen, Gry B
AU  - Boe-Hansen GB
AD  - School of Veterinary Sciences, The University of Queensland , Gatton, QLD , 
      Australia.
FAU - Piper, Emily
AU  - Piper E
AD  - School of Veterinary Sciences, The University of Queensland , Gatton, QLD , 
      Australia.
FAU - Lehnert, Sigrid A
AU  - Lehnert SA
AD  - CSIRO Agriculture Flagship, Queensland Bioscience Precinct , Brisbane, QLD , 
      Australia.
FAU - Guimaraes, Simone Eliza Facioni
AU  - Guimaraes SE
AD  - Animal Science Department, Universidade Federal de Vicosa , Vicosa, Minas Gerais 
      , Brazil.
FAU - Moore, Stephen Stewart
AU  - Moore SS
AD  - Queensland Alliance for Agriculture and Food Innovation, Centre for Animal 
      Science, The University of Queensland , Brisbane, QLD , Australia.
LA  - eng
PT  - Journal Article
DEP - 20161018
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC5067528
OTO - NOTNLM
OT  - bovine species
OT  - corpus luteum
OT  - folliculogenesis
OT  - intraovarian factors
OT  - quantitative real-time PCR
EDAT- 2016/11/03 06:00
MHDA- 2016/11/03 06:01
PMCR- 2016/01/01
CRDT- 2016/11/03 06:00
PHST- 2016/07/25 00:00 [received]
PHST- 2016/10/04 00:00 [accepted]
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2016/11/03 06:01 [medline]
PHST- 2016/11/03 06:00 [entrez]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2016.00094 [doi]
PST - epublish
SO  - Front Vet Sci. 2016 Oct 18;3:94. doi: 10.3389/fvets.2016.00094. eCollection 2016.