PMID- 27805413
OWN - NLM
STAT- MEDLINE
DCOM- 20161230
LR  - 20181202
IS  - 1480-3275 (Electronic)
IS  - 0008-4166 (Linking)
VI  - 62
IP  - 12
DP  - 2016 Dec
TI  - In situ identification and quantification of protein-hydrolyzing ruminal bacteria 
      associated with the digestion of barley and corn grain.
PG  - 1063-1067
AB  - In this study, BODIPY FL DQ casein staining combined with fluorescence in situ 
      hybridization (FISH) was used to detect and identify protein-hydrolyzing bacteria 
      within biofilms that produced active cell-surface-associated serine- and 
      metallo-proteases during the ruminal digestion of barley and corn grain in cows 
      fed barley-based diets at 2 different levels. A doublet coccoid bacterial 
      morphotype associated with barley and corn grain particles fluoresced after 
      BODIPY FL DQ casein staining. Bacteria with this morphotype accounted for 3%-10% 
      of the total bacteria attached to surface of cereal grain particles, possibly 
      indicative of an important role in the hydrolysis of the protein matrix within 
      the endosperm. However, the identity of these predominant proteolytic bacteria 
      could not be determined using FISH. Quantitative FISH revealed that known 
      proteolytic species, Prevotella ruminicola, Ruminobacter amylophilus, and 
      Butyrivibrio fibrisolvens, were attached to particles of various cultivars of 
      barley grain and corn, confirming their role in the proteolysis of cereal grains. 
      Differences in chemical composition among different barley cultivars did not 
      affect the composition of proteolytic bacterial populations. However, the 
      concentrate level in the basal diet did have an impact on the relative abundance 
      of proteolytic bacteria and thus possibly their overall contribution to the 
      proteolysis of cereal grains.
FAU - Xia, Yun
AU  - Xia Y
AD  - a Key laboratory of Special Biological Resource Development and Utilization of 
      Universities of Yunnan Province, Kunming University, Kunming, People's Republic 
      of China.
AD  - b Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 
      5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada.
FAU - Kong, Yunhong
AU  - Kong Y
AD  - a Key laboratory of Special Biological Resource Development and Utilization of 
      Universities of Yunnan Province, Kunming University, Kunming, People's Republic 
      of China.
FAU - Huang, Heping
AU  - Huang H
AD  - a Key laboratory of Special Biological Resource Development and Utilization of 
      Universities of Yunnan Province, Kunming University, Kunming, People's Republic 
      of China.
FAU - Yang, Hee Eun
AU  - Yang HE
AD  - b Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 
      5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada.
FAU - Forster, Robert
AU  - Forster R
AD  - b Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 
      5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada.
FAU - McAllister, Tim A
AU  - McAllister TA
AD  - b Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 
      5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada.
LA  - eng
PT  - Journal Article
DEP - 20160823
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
SB  - IM
MH  - Animal Feed
MH  - Animals
MH  - Bacteria/growth & development/*isolation & purification/metabolism
MH  - Cattle/*microbiology/physiology
MH  - Diet/veterinary
MH  - Digestion
MH  - Edible Grain/metabolism/microbiology
MH  - Female
MH  - *Hordeum/metabolism/microbiology
MH  - Hydrolysis
MH  - In Situ Hybridization, Fluorescence
MH  - Proteolysis
MH  - Rumen/*microbiology
MH  - *Zea mays/metabolism/microbiology
OTO - NOTNLM
OT  - FISH
OT  - bacteria
OT  - bacteries
OT  - barley
OT  - corn
OT  - mais
OT  - orge
OT  - proteases
OT  - proteases
OT  - rumen
EDAT- 2016/11/03 06:00
MHDA- 2016/12/31 06:00
CRDT- 2016/11/03 06:00
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2016/12/31 06:00 [medline]
PHST- 2016/11/03 06:00 [entrez]
AID - 10.1139/cjm-2016-0293 [doi]
PST - ppublish
SO  - Can J Microbiol. 2016 Dec;62(12):1063-1067. doi: 10.1139/cjm-2016-0293. Epub 2016 
      Aug 23.