PMID- 27806110
OWN - NLM
STAT- MEDLINE
DCOM- 20170622
LR  - 20231213
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 11
DP  - 2016
TI  - A Leader Intron of a Soybean Elongation Factor 1A (eEF1A) Gene Interacts with 
      Proximal Promoter Elements to Regulate Gene Expression in Synthetic Promoters.
PG  - e0166074
LID - 10.1371/journal.pone.0166074 [doi]
LID - e0166074
AB  - Introns, especially the first intron in the 5' untranslated region (5'UTR), can 
      significantly impact gene expression via intron-mediated enhancement (IME). In 
      this study, we demonstrate the leader intron of a soybean elongation factor 1A 
      (eEF1A) gene (GmScreamM8) was essential for the high activity of the native 
      promoter. Furthermore, the interaction of the GmScreamM8 leader intron with 
      regulatory element sequences from several soybean eEF1A promoters was studied 
      using synthetic promoters, which consisted of element tetramers upstream of a 
      core promoter used to regulate a green fluorescent protein (gfp) reporter gene. 
      Element tetramers, placed upstream of a GmScreamM8 core promoter, showed very 
      high activity using both transient expression in lima bean cotyledons and stable 
      expression in soybean hairy roots, only if the native leader intron was included, 
      suggesting an interaction between intronic sequences and promoter elements. 
      Partial deletions of the leader intron showed that a 222 bp intronic sequence 
      significantly contributed to very high levels of GFP expression. Generation of 
      synthetic intron variants with a monomeric or trimeric repeat of the 222 bp 
      intronic sequence, yielded almost two-fold higher expression compared to the 
      original intron, while partial deletion of the 222 bp intronic repeated sequence 
      significantly decreased gene expression, indicating that this intronic sequence 
      was essential for the intron-element interaction enhancement.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Department of Horticulture and Crop Science, The Ohio State University, Wooster, 
      Ohio, United States of America.
FAU - McHale, Leah K
AU  - McHale LK
AD  - Department of Horticulture and Crop Science, The Ohio State University, Columbus, 
      Ohio, United States of America.
FAU - Finer, John J
AU  - Finer JJ
AD  - Department of Horticulture and Crop Science, The Ohio State University, Wooster, 
      Ohio, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20161102
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Peptide Elongation Factors)
RN  - 0 (Plant Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Gene Expression Regulation, Plant
MH  - *Genes, Synthetic
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Introns
MH  - Peptide Elongation Factors/*genetics
MH  - Plant Proteins/genetics
MH  - Plants, Genetically Modified/metabolism
MH  - Promoter Regions, Genetic
MH  - Glycine max/*genetics
PMC - PMC5091777
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/11/03 06:00
MHDA- 2017/06/24 06:00
PMCR- 2016/11/02
CRDT- 2016/11/03 06:00
PHST- 2016/09/19 00:00 [received]
PHST- 2016/10/21 00:00 [accepted]
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2017/06/24 06:00 [medline]
PHST- 2016/11/03 06:00 [entrez]
PHST- 2016/11/02 00:00 [pmc-release]
AID - PONE-D-16-37640 [pii]
AID - 10.1371/journal.pone.0166074 [doi]
PST - epublish
SO  - PLoS One. 2016 Nov 2;11(11):e0166074. doi: 10.1371/journal.pone.0166074. 
      eCollection 2016.