PMID- 27807544
OWN - NLM
STAT- MEDLINE
DCOM- 20170609
LR  - 20190723
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2016
DP  - 2016
TI  - Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are 
      Different from Those Observed in Type 2 Diabetes Mellitus.
PG  - 3876764
LID - 3876764
AB  - Background. Type 2 diabetes mellitus (T2DM) is determined by genetic and 
      environmental factors. There have been many studies on the relationship between 
      the composition of the gastrointestinal bacterial flora, T2DM, and obesity. There 
      are no data, however, on the gut microbiome structure in monogenic forms of the 
      disease including Maturity Onset Diabetes of the Young (MODY). Methods. The aim 
      of the investigation was to compare the qualitative parameters of the colonic 
      flora in patients with HNF1A-MODY and T2DM and healthy individuals. 16S 
      sequencing of bacterial DNA isolated from the collected fecal samples using the 
      MiSeq platform was performed. Results. There were significant between-group 
      differences in the bacterial profile. At the phylum level, the amount of 
      Proteobacteria was higher (p = 0.0006) and the amount of Bacteroidetes was lower 
      (p = 0.0005) in T2DM group in comparison to the control group. In HNF1A-MODY 
      group, the frequency of Bacteroidetes was lower than in the control group (p = 
      0.0143). At the order level, Turicibacterales was more abundant in HNF1A-MODY 
      group than in T2DM group. Conclusions. It appears that there are differences in 
      the gut microbiome composition between patients with HNF1A-MODY and type 2 
      diabetes. Further investigation on this matter should be conducted.
FAU - Mrozinska, Sandra
AU  - Mrozinska S
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 15 
      Kopernika Street, 31-501 Krakow, Poland; University Hospital, 36 Kopernika 
      Street, 31-501 Krakow, Poland.
FAU - Radkowski, Piotr
AU  - Radkowski P
AD  - Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c 
      Kopernika Street, 31-034 Krakow, Poland.
FAU - Gosiewski, Tomasz
AU  - Gosiewski T
AD  - Department of Microbiology, Jagiellonian University Medical College, 18 Czysta 
      Street, 31-121 Krakow, Poland.
FAU - Szopa, Magdalena
AU  - Szopa M
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 15 
      Kopernika Street, 31-501 Krakow, Poland; University Hospital, 36 Kopernika 
      Street, 31-501 Krakow, Poland.
FAU - Bulanda, Malgorzata
AU  - Bulanda M
AD  - Department of Microbiology, Jagiellonian University Medical College, 18 Czysta 
      Street, 31-121 Krakow, Poland.
FAU - Ludwig-Galezowska, Agnieszka H
AU  - Ludwig-Galezowska AH
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 15 
      Kopernika Street, 31-501 Krakow, Poland; Center for Medical Genomics OMICRON, 
      Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Krakow, 
      Poland.
FAU - Morawska, Iwona
AU  - Morawska I
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 15 
      Kopernika Street, 31-501 Krakow, Poland; University Hospital, 36 Kopernika 
      Street, 31-501 Krakow, Poland.
FAU - Sroka-Oleksiak, Agnieszka
AU  - Sroka-Oleksiak A
AD  - Department of Microbiology, Jagiellonian University Medical College, 18 Czysta 
      Street, 31-121 Krakow, Poland.
FAU - Matejko, Bartlomiej
AU  - Matejko B
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 15 
      Kopernika Street, 31-501 Krakow, Poland.
FAU - Kapusta, Przemyslaw
AU  - Kapusta P
AD  - Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c 
      Kopernika Street, 31-034 Krakow, Poland.
FAU - Salamon, Dominika
AU  - Salamon D
AD  - Department of Microbiology, Jagiellonian University Medical College, 18 Czysta 
      Street, 31-121 Krakow, Poland.
FAU - Malecki, Maciej T
AU  - Malecki MT
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 15 
      Kopernika Street, 31-501 Krakow, Poland; University Hospital, 36 Kopernika 
      Street, 31-501 Krakow, Poland.
FAU - Wolkow, Pawel
AU  - Wolkow P
AD  - Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c 
      Kopernika Street, 31-034 Krakow, Poland.
FAU - Klupa, Tomasz
AU  - Klupa T
AUID- ORCID: 0000-0002-7199-4079
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 15 
      Kopernika Street, 31-501 Krakow, Poland; University Hospital, 36 Kopernika 
      Street, 31-501 Krakow, Poland.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20161011
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (RNA, Ribosomal, 16S)
RN  - Maturity-Onset Diabetes of the Young, Type 3
SB  - IM
MH  - Adult
MH  - Bacterial Typing Techniques
MH  - Bacteroidetes/genetics/isolation & purification
MH  - Case-Control Studies
MH  - Colon/*microbiology
MH  - Diabetes Mellitus, Type 2/*microbiology
MH  - Female
MH  - Gastrointestinal Microbiome/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Phylogeny
MH  - Proteobacteria/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/genetics
PMC - PMC5078663
EDAT- 2016/11/04 06:00
MHDA- 2017/06/10 06:00
PMCR- 2016/10/11
CRDT- 2016/11/04 06:00
PHST- 2016/06/07 00:00 [received]
PHST- 2016/08/22 00:00 [revised]
PHST- 2016/08/30 00:00 [accepted]
PHST- 2016/11/04 06:00 [pubmed]
PHST- 2017/06/10 06:00 [medline]
PHST- 2016/11/04 06:00 [entrez]
PHST- 2016/10/11 00:00 [pmc-release]
AID - 10.1155/2016/3876764 [doi]
PST - ppublish
SO  - J Diabetes Res. 2016;2016:3876764. doi: 10.1155/2016/3876764. Epub 2016 Oct 11.