PMID- 27809943
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20170522
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 116
IP  - 9
DP  - 2016 Nov
TI  - Use of molecular biomarkers to estimate manganese requirements for broiler 
      chickens from 22 to 42 d of age.
PG  - 1512-1518
AB  - The present study was carried out to evaluate dietary Mn requirements of broilers 
      from 22 to 42 d of age using molecular biomarkers. Chickens were fed a 
      conventional basal maize-soyabean meal diet supplemented with Mn as Mn sulphate 
      in graded concentrations of 20 mg Mn/kg from 0 to 140 mg Mn/kg of diet for 21 d 
      (from 22 to 42 d of age). The Mn response curves were fitted for ten parameters 
      including heart Mn-containing superoxide dismutase (MnSOD) mRNA and its protein 
      expression levels and the DNA-binding activities of specificity protein 1 (Sp1) 
      and activating protein-2 (AP-2). Heart MnSOD mRNA and protein expression levels 
      showed significant quadratic responses (P<0.01), and heart MnSOD activity showed 
      a broken-line response (P<0.01), whereas Mn content and DNA-binding activities of 
      Sp1 and AP-2 in the heart displayed linear responses (P<0.01) to dietary Mn 
      concentrations, respectively. The estimates of dietary Mn requirements were 101, 
      104 and 94 mg/kg for full expressions of MnSOD mRNA level, MnSOD protein level 
      and MnSOD activity in the heart, respectively. Our findings indicate that heart 
      MnSOD mRNA expression level is a more reliable indicator than heart MnSOD protein 
      expression level and its activity for the evaluation of Mn requirement of 
      broilers, and about 100 mg Mn/kg of diet is required for the full expression of 
      heart MnSOD in broilers fed the conventional basal maize-soyabean meal diet from 
      22 to 42 d of age.
FAU - Lu, Lin
AU  - Lu L
AD  - 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy 
      of Agricultural Sciences (CAAS),Beijing 100193,People's Republic of China.
FAU - Chang, Bin
AU  - Chang B
AD  - 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy 
      of Agricultural Sciences (CAAS),Beijing 100193,People's Republic of China.
FAU - Liao, Xiudong
AU  - Liao X
AD  - 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy 
      of Agricultural Sciences (CAAS),Beijing 100193,People's Republic of China.
FAU - Wang, Runlian
AU  - Wang R
AD  - 2Department of Animal Science,Guangdong Ocean University,Zhanjiang 
      524088,People's Republic of China.
FAU - Zhang, Liyang
AU  - Zhang L
AD  - 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy 
      of Agricultural Sciences (CAAS),Beijing 100193,People's Republic of China.
FAU - Luo, Xugang
AU  - Luo X
AD  - 1Mineral Nutrition Research Division,Institute of Animal Science,Chinese Academy 
      of Agricultural Sciences (CAAS),Beijing 100193,People's Republic of China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20161104
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Avian Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Manganese Compounds)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Sulfates)
RN  - 0 (Transcription Factor AP-2)
RN  - 42Z2K6ZL8P (Manganese)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - W00LYS4T26 (manganese sulfate)
SB  - IM
MH  - Animals
MH  - Avian Proteins/chemistry/genetics/*metabolism
MH  - Biomarkers/metabolism
MH  - Chickens/growth & development/*physiology
MH  - China
MH  - Energy Intake
MH  - *Gene Expression Regulation, Enzymologic
MH  - Heart/growth & development
MH  - Male
MH  - Manganese/*administration & dosage/analysis/metabolism
MH  - Manganese Compounds/administration & dosage
MH  - Myocardium/enzymology/*metabolism
MH  - *Nutritional Requirements
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Reproducibility of Results
MH  - Sp1 Transcription Factor/chemistry/metabolism
MH  - Sulfates/administration & dosage
MH  - Superoxide Dismutase/chemistry/genetics/*metabolism
MH  - Transcription Factor AP-2/chemistry/metabolism
MH  - Weight Gain
OTO - NOTNLM
OT  - AP-2 activating protein-2
OT  - MnSOD manganese-containing superoxide dismutase
OT  - Sp1 specificity protein 1
OT  - Broilers
OT  - Dietary requirements
OT  - Gene expression
OT  - Manganese
OT  - Manganese-containing superoxide dismutase
EDAT- 2016/11/05 06:00
MHDA- 2017/05/23 06:00
CRDT- 2016/11/05 06:00
PHST- 2016/11/05 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
PHST- 2016/11/05 06:00 [entrez]
AID - S0007114516003640 [pii]
AID - 10.1017/S0007114516003640 [doi]
PST - ppublish
SO  - Br J Nutr. 2016 Nov;116(9):1512-1518. doi: 10.1017/S0007114516003640. Epub 2016 
      Nov 4.