PMID- 27809951
OWN - NLM
STAT- MEDLINE
DCOM- 20170915
LR  - 20170915
IS  - 1475-2670 (Electronic)
IS  - 0007-4853 (Linking)
VI  - 107
IP  - 3
DP  - 2017 Jun
TI  - Molecular characterization, expression analysis and RNAi knock-down of elongation 
      factor 1alpha and 1gamma from Nilaparvata lugens and its yeast-like symbiont.
PG  - 303-312
LID - 10.1017/S0007485316000882 [doi]
AB  - In the present paper, four cDNAs encoding the alpha and gamma subunits of 
      elongation factor 1 (EF-1) were cloned and sequenced from Nilaparvata lugens, 
      named NlEF-1alpha, NlEF-1gamma, and its yeast-like symbiont (YLS), named YsEF-1alpha and 
      YsEF-1gamma, respectively. Comparisons with sequences from other species indicated a 
      greater conservation for EF-1alpha than for EF-1gamma. NlEF-1alpha has two identical copies. 
      The deduced amino acid sequence homology of NlEF-1alpha and NlEF-1gamma is 96 and 64%, 
      respectively, compared with Homalodisca vitripennis and Locusta migratoria. The 
      deduced amino acid sequence homology of YsEF-1alpha and YsEF-1gamma is 96 and 74%, 
      respectively, compared with Metarhizium anisopliae and Ophiocordyceps sinensis. 
      Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis 
      revealed that the expression level of NlEF-1alpha and NlEF-1gamma mRNA in hemolymph, 
      ovary, fat body and salivary glands were higher than the midgut and leg tissue. 
      YsEF-1alpha and YsEF-1gamma was highly expressed in fat body. The expression level of 
      NlEF-1alpha was higher than that of NlEF-1gamma. Through RNA interference (RNAi) of the 
      two genes, the mortality of nymph reached 92.2% at the 11th day after treatment 
      and the ovarian development was severely hindered. The RT-qPCR analysis verified 
      the correlation between mortality, sterility and the down-regulation of the 
      target genes. The expression and synthesis of vitellogenin (Vg) protein in 
      insects injected with NlEF-1alpha and NlEF-1gamma double-stranded RNA (dsRNA) was 
      significantly lower than control groups. Attempts to knockdown the YsEF-1 genes 
      in the YLS was unsuccessful. However, the phenotype of N. lugens injected with 
      YsEF-1alpha dsRNA was the same as that injected with NlEF-1alpha dsRNA, possibly due to 
      the high similarity (up to 71.9%) in the nucleotide sequences between NlEF-1alpha and 
      YsEF-1alpha. We demonstrated that partial silencing of NlEF-1alpha and NlEF-1gamma genes 
      caused lethal and sterility effect on N. lugens. NlEF-1gamma shares low identity with 
      that of other insects and therefore it could be a potential target for RNAi-based 
      pest management.
FAU - Wang, W X
AU  - Wang WX
AD  - State Key Laboratory of Rice Biology,China National Rice Research 
      Institute,Tiyuchang Road 359, Hangzhou, Zhejiang, 310006,People's Republic of 
      China.
FAU - Zhu, T H
AU  - Zhu TH
AD  - College of Biological and Environmental Engineering,Zhejiang University of 
      Technology,Chaowang Road,Hangzhou,Zhejiang, 310014,People's Republic of China.
FAU - Li, K L
AU  - Li KL
AD  - State Key Laboratory of Rice Biology,China National Rice Research 
      Institute,Tiyuchang Road 359, Hangzhou, Zhejiang, 310006,People's Republic of 
      China.
FAU - Chen, L F
AU  - Chen LF
AD  - State Key Laboratory of Rice Biology,China National Rice Research 
      Institute,Tiyuchang Road 359, Hangzhou, Zhejiang, 310006,People's Republic of 
      China.
FAU - Lai, F X
AU  - Lai FX
AD  - State Key Laboratory of Rice Biology,China National Rice Research 
      Institute,Tiyuchang Road 359, Hangzhou, Zhejiang, 310006,People's Republic of 
      China.
FAU - Fu, Q
AU  - Fu Q
AD  - State Key Laboratory of Rice Biology,China National Rice Research 
      Institute,Tiyuchang Road 359, Hangzhou, Zhejiang, 310006,People's Republic of 
      China.
LA  - eng
PT  - Journal Article
DEP - 20161104
PL  - England
TA  - Bull Entomol Res
JT  - Bulletin of entomological research
JID - 2984715R
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Peptide Elongation Factor 1)
SB  - IM
MH  - Animals
MH  - DNA, Complementary/genetics/metabolism
MH  - Female
MH  - Fungal Proteins/chemistry/*genetics/metabolism
MH  - Gene Expression
MH  - Hemiptera/genetics/growth & development/microbiology/*physiology
MH  - Insect Proteins/chemistry/*genetics/metabolism
MH  - Male
MH  - Nymph/genetics/growth & development/physiology
MH  - Organ Specificity
MH  - Peptide Elongation Factor 1/chemistry/*genetics/metabolism
MH  - RNA Interference
MH  - Sequence Analysis, Protein
MH  - Sequence Homology, Amino Acid
MH  - Symbiosis
MH  - Yeasts/genetics/*physiology
OTO - NOTNLM
OT  - Nilaparvata lugens
OT  - RNAi
OT  - elongation factor 1 alpha
OT  - elongation factor 1 gamma
OT  - yeast-like symbionts
EDAT- 2016/11/05 06:00
MHDA- 2017/09/16 06:00
CRDT- 2016/11/05 06:00
PHST- 2016/11/05 06:00 [pubmed]
PHST- 2017/09/16 06:00 [medline]
PHST- 2016/11/05 06:00 [entrez]
AID - S0007485316000882 [pii]
AID - 10.1017/S0007485316000882 [doi]
PST - ppublish
SO  - Bull Entomol Res. 2017 Jun;107(3):303-312. doi: 10.1017/S0007485316000882. Epub 
      2016 Nov 4.