PMID- 27811389
OWN - NLM
STAT- MEDLINE
DCOM- 20171221
LR  - 20181202
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Print)
IS  - 1748-6041 (Linking)
VI  - 11
IP  - 6
DP  - 2016 Nov 4
TI  - Hydrogel fibers encapsulating human stem cells in an injectable calcium phosphate 
      scaffold for bone tissue engineering.
PG  - 065008
AB  - Human induced pluripotent stem cells (hiPSCs), human embryonic stem cells (hESCs) 
      and human umbilical cord mesenchymal stem cells (hUCMSCs) are exciting cell 
      sources for use in regenerative medicine. There have been no reports on long 
      hydrogel fibers encapsulating stem cells inside an injectable calcium phosphate 
      cement (CPC) scaffold for bone tissue engineering. The objectives of this study 
      were: (1) to develop a novel injectable CPC construct containing hydrogel fibers 
      encapsulating cells for bone engineering, and (2) to investigate and compare cell 
      viability, proliferation and osteogenic differentiation of hiPSC-MSCs, hESC-MSCs 
      and hUCMSCs in injectable CPC. The pastes encapsulating the stem cells were fully 
      injectable under a small injection force, and the injection did not harm the 
      cells, compared with non-injected cells (p  >  0.1). The mechanical properties of 
      the stem cell-CPC construct were much better than those of previous injectable 
      polymers and hydrogels for cell delivery. The hiPSC-MSCs, hESC-MSCs and hUCMSCs 
      in hydrogel fibers in CPC had excellent proliferation and osteogenic 
      differentiation. All three cell types yielded high alkaline phosphatase, 
      runt-related transcription factor, collagen I and osteocalcin expression 
      (mean  +/-  SD; n  =  6). Cell-synthesized minerals increased substantially with 
      time (p  <  0.05), with no significant difference among the three types of cells 
      (p  >  0.1). Mineralization by hiPSC-MSCs, hESC-MSCs and hUCMSCs in CPC at 14 d 
      was 13-fold that at 1 d. In conclusion, all three types of cells (hiPSC-MSCs, 
      hESC-MSCs and hUCMSCs) in a CPC scaffold showed high potential for bone tissue 
      engineering, and the novel injectable CPC construct with cell-encapsulating 
      hydrogel fibers is promising for enhancing bone regeneration in dental, 
      craniofacial and orthopedic applications.
FAU - Wang, Lin
AU  - Wang L
AD  - VIP Integrated Department, Stomatological Hospital of Jilin University, 
      Changchun, Jilin 130011, People's Republic of China. Department of Endodontics, 
      Periodontics and Prosthodontics, University of Maryland School of Dentistry, 
      Baltimore, MD 21201, USA.
FAU - Wang, Ping
AU  - Wang P
FAU - Weir, Michael D
AU  - Weir MD
FAU - Reynolds, Mark A
AU  - Reynolds MA
FAU - Zhao, Liang
AU  - Zhao L
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R21 DE022625/DE/NIDCR NIH HHS/United States
PT  - Journal Article
DEP - 20161104
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Alginates)
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Hydrogels)
RN  - 0 (Polymers)
RN  - 104982-03-8 (Osteocalcin)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 97Z1WI3NDX (calcium phosphate)
RN  - K4C08XP666 (tricalcium phosphate)
SB  - IM
MH  - Alginates/chemistry
MH  - Bone Cements/pharmacology
MH  - Bone Regeneration/drug effects
MH  - Bone and Bones/*chemistry
MH  - Calcium Phosphates/*chemistry
MH  - Cell Differentiation/drug effects
MH  - Cell Lineage
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Glucuronic Acid/chemistry
MH  - Hexuronic Acids/chemistry
MH  - Humans
MH  - Hydrogels/*chemistry
MH  - Osteocalcin/metabolism
MH  - Osteogenesis/drug effects
MH  - Polymers/chemistry
MH  - Stem Cells/*cytology
MH  - Stress, Mechanical
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
PMC - PMC5382017
MID - NIHMS828843
EDAT- 2016/11/05 06:00
MHDA- 2017/12/22 06:00
PMCR- 2017/11/04
CRDT- 2016/11/05 06:00
PHST- 2016/11/05 06:00 [pubmed]
PHST- 2017/12/22 06:00 [medline]
PHST- 2016/11/05 06:00 [entrez]
PHST- 2017/11/04 00:00 [pmc-release]
AID - 10.1088/1748-6041/11/6/065008 [doi]
PST - epublish
SO  - Biomed Mater. 2016 Nov 4;11(6):065008. doi: 10.1088/1748-6041/11/6/065008.