PMID- 27812843
OWN - NLM
STAT- MEDLINE
DCOM- 20170425
LR  - 20190816
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 40
IP  - 1
DP  - 2017 Feb
TI  - Porphyromonas gingivalis Lipopolysaccharide Induces a Pro-inflammatory Human 
      Gingival Fibroblast Phenotype.
PG  - 144-153
LID - 10.1007/s10753-016-0463-7 [doi]
AB  - Human gingival fibroblasts (HGFs) are the major constituents of the gingival 
      tissues responsible for the synthesis and degradation of the connective tissue 
      while actively participating in immune reactions and inflammation. The aim of 
      this study was to test the impact of lipopolysaccharide (LPS) from Porphyromonas 
      gingivalis (P. gingivalis) on human gingival fibroblasts. Human gingival 
      fibroblasts were treated with different P. gingivalis LPS concentrations. Cell 
      survival rate was evaluated with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl 
      tetrazolium bromide (MTT) after 24 h. Cell proliferation was determined by 
      counting cells on days 3 and 12. Expression of matrix metalloproteinases (MMPs), 
      tissue inhibitors of MMPs (TIMPs), and pro-inflammatory cytokine transcripts in 
      HGFs was determined by quantitative PCR (Q-PCR) analysis on days 3 and 8. P. 
      gingivalis LPS decreased cell proliferation on day 3 (p < 0.05) compared to the 
      control group without significantly impacting the cell survival (p > 0.05).The 
      experiments showed that P. gingivalis LPS dose-dependently and differentially 
      modulated the expression of MMP-1, 2, and 3 and TIMP-1 and 2 on days 3 and 8. 
      TIMP-1 expression was significantly induced in P. gingivalis LPS-treated cells 
      while TIMP-2 was increased in response to 10 and 30 ng/ml of LPS on day 3. P. 
      gingivalis LPS induced up-regulation of MMP-1/TIMP-1 ratio on day 3 and increased 
      MMP-2/TIMP-2 ratio on day 8 dose-dependently. Expression of interleukin (IL)-6 
      and IL-8 was stimulated at higher concentrations (1000 and 3000 ng/ml) of LPS. 
      These findings demonstrate that P. gingivalis LPS suppresses cell proliferation 
      and leads to increased pro-inflammatory changes in HGFs, suggesting that P. 
      gingivalis LPS-induced modification of phenotypic and inflammatory 
      characteristics in HGF could potentially be a pathogenic mechanism underlying the 
      tissue destruction.
FAU - Bozkurt, S Buket
AU  - Bozkurt SB
AD  - Research Center of Faculty of Dentistry, Selcuk University, Konya, Turkey. 
      buketbozkurt@yahoo.com.
FAU - Hakki, Sema S
AU  - Hakki SS
AD  - Faculty of Dentistry, Department of Periodontology, Selcuk University, Konya, 
      Turkey.
FAU - Hakki, Erdogan E
AU  - Hakki EE
AD  - Faculty of Agriculture, Department of Soil Science & Plant Nutrition, Selcuk 
      University, Konya, Turkey.
FAU - Durak, Yusuf
AU  - Durak Y
AD  - Faculty of Science, Department of Biology, Selcuk University, Konya, Turkey.
FAU - Kantarci, Alpdogan
AU  - Kantarci A
AD  - Department of Periodontology, The Forsyth Institute, Boston, MA, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (CXCL8 protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (TIMP2 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Dose-Response Relationship, Drug
MH  - Fibroblasts/microbiology/*pathology
MH  - Gingiva/microbiology/*pathology
MH  - Humans
MH  - Inflammation/chemically induced/*pathology
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Matrix Metalloproteinases/metabolism
MH  - Porphyromonas gingivalis/chemistry
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/metabolism
OTO - NOTNLM
OT  - Porphyromonas gingivalis
OT  - cytokine
OT  - fibroblast
OT  - lipopolysaccharide
EDAT- 2016/11/05 06:00
MHDA- 2017/04/26 06:00
CRDT- 2016/11/05 06:00
PHST- 2016/11/05 06:00 [pubmed]
PHST- 2017/04/26 06:00 [medline]
PHST- 2016/11/05 06:00 [entrez]
AID - 10.1007/s10753-016-0463-7 [pii]
AID - 10.1007/s10753-016-0463-7 [doi]
PST - ppublish
SO  - Inflammation. 2017 Feb;40(1):144-153. doi: 10.1007/s10753-016-0463-7.