PMID- 27813686
OWN - NLM
STAT- MEDLINE
DCOM- 20170131
LR  - 20181202
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 36
IP  - 1
DP  - 2017 Jan
TI  - The Telomerase Activity of Selenium-Induced Human Umbilical Cord Mesenchymal Stem 
      Cells Is Associated with Different Levels of c-Myc and p53 Expression.
PG  - 34-41
LID - 10.1089/dna.2016.3411 [doi]
AB  - Selenium-as a trace element-is nutritionally essential for humans. It prevents 
      cancerous growth by inhibiting the telomerase activity but the mechanism involved 
      in regulation of telomerase activity in normal telomerase-positive cells remains 
      to be elucidated. Here, we find out whether the effect of sodium selenite and 
      selenomethionine on telomerase activity in human umbilical cord-derived 
      mesenchymal stem cells (hUCMSCs) is associated with different levels of c-Myc and 
      p53 expression. The use of different staining methods including ethidium 
      bromide/acridine orange and DAPI in addition to telomeric repeat amplification 
      protocol assay and real-time PCR indicated that different forms of selenium have 
      opposite impacts on c-Myc and p53 expressions in both hUCMSCs and AGS, a gastric 
      adenocarcinoma cell line, as a positive control. Our findings suggest that the 
      signaling pathways involved in the regulation of telomerase activity in malignant 
      and normal telomerase-positive cell types are somewhat different, at least on the 
      c-Myc and P53 expression levels.
FAU - Hosseinzadeh Anvar, Leila
AU  - Hosseinzadeh Anvar L
AD  - 1 Research Laboratory for Embryology and Stem Cells, Department of Anatomical 
      Sciences and Pathology, Faculty of Medicine, Ardabil University of Medical 
      Sciences , Ardabil, Iran .
AD  - 2 Laboratory of Medical Genetics, Imam Khomeini Hospital, Ardabil University of 
      Medical Sciences , Ardabil, Iran .
FAU - Hosseini-Asl, Saeid
AU  - Hosseini-Asl S
AD  - 2 Laboratory of Medical Genetics, Imam Khomeini Hospital, Ardabil University of 
      Medical Sciences , Ardabil, Iran .
FAU - Mohammadzadeh-Vardin, Mohammad
AU  - Mohammadzadeh-Vardin M
AD  - 1 Research Laboratory for Embryology and Stem Cells, Department of Anatomical 
      Sciences and Pathology, Faculty of Medicine, Ardabil University of Medical 
      Sciences , Ardabil, Iran .
FAU - Sagha, Mohsen
AU  - Sagha M
AD  - 1 Research Laboratory for Embryology and Stem Cells, Department of Anatomical 
      Sciences and Pathology, Faculty of Medicine, Ardabil University of Medical 
      Sciences , Ardabil, Iran .
LA  - eng
PT  - Journal Article
DEP - 20161104
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 964MRK2PEL (Selenomethionine)
RN  - EC 2.7.7.49 (Telomerase)
RN  - HIW548RQ3W (Sodium Selenite)
SB  - IM
MH  - Cell Survival/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*drug effects/metabolism
MH  - Proto-Oncogene Proteins c-myc/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Selenomethionine/*pharmacology
MH  - Sodium Selenite/*pharmacology
MH  - Telomerase/*metabolism
MH  - Tumor Suppressor Protein p53/*genetics
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - P53
OT  - c-Myc
OT  - hUCMSCs
OT  - selenium
OT  - telomerase activity
EDAT- 2016/11/05 06:00
MHDA- 2017/02/01 06:00
CRDT- 2016/11/05 06:00
PHST- 2016/11/05 06:00 [pubmed]
PHST- 2017/02/01 06:00 [medline]
PHST- 2016/11/05 06:00 [entrez]
AID - 10.1089/dna.2016.3411 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2017 Jan;36(1):34-41. doi: 10.1089/dna.2016.3411. Epub 2016 Nov 4.