PMID- 27815212
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20180823
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 439
DP  - 2017 Jan 5
TI  - Claudin-31 contributes to corticosteroid-induced alterations in the barrier 
      properties of the gill epithelium.
PG  - 457-466
LID - S0303-7207(16)30445-2 [pii]
LID - 10.1016/j.mce.2016.10.034 [doi]
AB  - The contribution of Claudin-31 (Cldn-31) to corticosteroid-induced tightening of 
      the trout gill epithelium was examined using a primary cultured model 
      preparation. Cldn-31 is a  approximately 23 kDa protein that localizes to the periphery of gill 
      epithelial cells and diffusely in select gill cells that are 
      Na(+)-K(+)-ATPase-immunoreactive. Transcriptional knockdown (KD) of cldn-31 
      reduced Cldn-31 abundance and increased epithelium permeability. Under simulated 
      in vivo conditions (apical freshwater), cldn-31 KD increased net ion flux rates 
      ( identical with efflux). Cortisol treatment increased Cldn-31 abundance and decreased 
      epithelium permeability. This tightening effect was diminished, but not 
      eliminated, by cldn-31 KD, most likely due to other cortisol-sensitive TJ 
      proteins that were transcriptionally unperturbed or enhanced in cortisol-treated 
      cldn-31 KD preparations. However, cldn-31 KD abolished a cortisol-induced 
      increase in Cldn-8d abundance, which may contribute to compromised cldn-31 KD 
      epithelium permeability. Data suggest an important barrier function for Cldn-31 
      and an integral role for Cldn-31 in corticosteroid-induced gill epithelium 
      tightening.
CI  - Copyright A(c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Kolosov, Dennis
AU  - Kolosov D
AD  - Department of Biology, York University, Toronto, ON, M3J 1P3, Canada.
FAU - Donini, Andrew
AU  - Donini A
AD  - Department of Biology, York University, Toronto, ON, M3J 1P3, Canada.
FAU - Kelly, Scott P
AU  - Kelly SP
AD  - Department of Biology, York University, Toronto, ON, M3J 1P3, Canada. Electronic 
      address: spk@yorku.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161101
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Claudins)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Adrenal Cortex Hormones/*pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cells, Cultured
MH  - Claudins/chemistry/*metabolism
MH  - Electric Impedance
MH  - Epithelium/drug effects/*metabolism
MH  - Gene Knockdown Techniques
MH  - Gills/cytology/*metabolism
MH  - Hydrocortisone/pharmacology
MH  - Models, Biological
MH  - Oncorhynchus mykiss/metabolism
MH  - Transcription, Genetic/drug effects
OTO - NOTNLM
OT  - Cortisol
OT  - Fish
OT  - Knockdown
OT  - Osmoregulation
OT  - Paracellular permeability
OT  - Tight junction
EDAT- 2016/11/07 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/11/06 06:00
PHST- 2016/07/29 00:00 [received]
PHST- 2016/10/24 00:00 [revised]
PHST- 2016/10/31 00:00 [accepted]
PHST- 2016/11/07 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/11/06 06:00 [entrez]
AID - S0303-7207(16)30445-2 [pii]
AID - 10.1016/j.mce.2016.10.034 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2017 Jan 5;439:457-466. doi: 10.1016/j.mce.2016.10.034. Epub 
      2016 Nov 1.