PMID- 27815426
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20180124
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 197
IP  - 11
DP  - 2016 Dec 1
TI  - GATA1-Deficient Dendritic Cells Display Impaired CCL21-Dependent Migration toward 
      Lymph Nodes Due to Reduced Levels of Polysialic Acid.
PG  - 4312-4324
AB  - Dendritic cells (DCs) play a pivotal role in the regulation of the immune 
      response. DC development and activation is finely orchestrated through 
      transcriptional programs. GATA1 transcription factor is required for murine DC 
      development, and data suggest that it might be involved in the fine-tuning of the 
      life span and function of activated DCs. We generated DC-specific Gata1 knockout 
      mice (Gata1-KO(DC)), which presented a 20% reduction of splenic DCs, partially 
      explained by enhanced apoptosis. RNA sequencing analysis revealed a number of 
      deregulated genes involved in cell survival, migration, and function. DC 
      migration toward peripheral lymph nodes was impaired in Gata1-KO(DC) mice. 
      Migration assays performed in vitro showed that this defect was selective for 
      CCL21, but not CCL19. Interestingly, we show that Gata1-KO(DC) DCs have reduced 
      polysialic acid levels on their surface, which is a known determinant for the 
      proper migration of DCs toward CCL21.
CI  - Copyright (c) 2016 by The American Association of Immunologists, Inc.
FAU - Scheenstra, Maaike R
AU  - Scheenstra MR
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
FAU - De Cuyper, Iris M
AU  - De Cuyper IM
AUID- ORCID: 0000-0001-8862-8000
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
FAU - Branco-Madeira, Filipe
AU  - Branco-Madeira F
AUID- ORCID: 0000-0001-5245-9735
AD  - Laboratory of Immunoregulation, VIB, Ghent 9052, Belgium.
AD  - Department of Internal Medicine, Ghent University, Ghent 9000, Belgium.
FAU - de Bleser, Pieter
AU  - de Bleser P
AUID- ORCID: 0000-0003-4762-8770
AD  - Data Mining and Modeling for Biomedicine, Department of Biomedical Molecular 
      Biology, VIB Inflammation Research Center, Ghent University, Ghent 9052, Belgium.
FAU - Kool, Mirjam
AU  - Kool M
AUID- ORCID: 0000-0003-1436-3876
AD  - Laboratory of Immunoregulation, VIB, Ghent 9052, Belgium.
FAU - Meinders, Marjolein
AU  - Meinders M
AUID- ORCID: 0000-0002-7124-0832
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
FAU - Hoogenboezem, Mark
AU  - Hoogenboezem M
AD  - Department of Molecular Cell Biology, Sanquin Research and Landsteiner 
      Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, 
      the Netherlands.
FAU - Mul, Erik
AU  - Mul E
AUID- ORCID: 0000-0002-8234-9972
AD  - Department of Molecular Cell Biology, Sanquin Research and Landsteiner 
      Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, 
      the Netherlands.
FAU - Wolkers, Monika C
AU  - Wolkers MC
AUID- ORCID: 0000-0003-3242-1363
AD  - Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
FAU - Salerno, Fiamma
AU  - Salerno F
AUID- ORCID: 0000-0003-1634-947X
AD  - Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
FAU - Nota, Benjamin
AU  - Nota B
AUID- ORCID: 0000-0001-7500-5760
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
FAU - Saeys, Yvan
AU  - Saeys Y
AUID- ORCID: 0000-0002-0415-1506
AD  - Data Mining and Modeling for Biomedicine, Department of Biomedical Molecular 
      Biology, VIB Inflammation Research Center, Ghent University, Ghent 9052, Belgium.
FAU - Klarenbeek, Sjoerd
AU  - Klarenbeek S
AUID- ORCID: 0000-0002-1891-1940
AD  - Experimental Animal Pathology, The Netherlands Cancer Institute, Amsterdam 
      1066CX, the Netherlands.
FAU - van IJcken, Wilfred F J
AU  - van IJcken WF
AUID- ORCID: 0000-0002-0421-8301
AD  - Center for Biomics, Erasmus MC, Rotterdam 3015 CN, the Netherlands.
FAU - Hammad, Hamida
AU  - Hammad H
AD  - Laboratory of Immunoregulation, VIB, Ghent 9052, Belgium.
AD  - Department of Internal Medicine, Ghent University, Ghent 9000, Belgium.
FAU - Philipsen, Sjaak
AU  - Philipsen S
AUID- ORCID: 0000-0002-3690-1201
AD  - Department of Cell Biology, Erasmus MC, Rotterdam 3015 CN, the Netherlands.
FAU - van den Berg, Timo K
AU  - van den Berg TK
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
FAU - Kuijpers, Taco W
AU  - Kuijpers TW
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands.
AD  - Department of Pediatric Hematology, Immunology and Infectious Disease, Emma 
      Children's Hospital, Academic Medical Center, University of Amsterdam, Amsterdam 
      1105AZ, the Netherlands.
FAU - Lambrecht, Bart N
AU  - Lambrecht BN
AD  - Laboratory of Immunoregulation, VIB, Ghent 9052, Belgium.
AD  - Department of Internal Medicine, Ghent University, Ghent 9000, Belgium.
AD  - Department of Pulmonary Medicine, Erasmus MC, Rotterdam 3015 CN, the Netherlands; 
      and.
FAU - Gutierrez, Laura
AU  - Gutierrez L
AUID- ORCID: 0000-0001-8443-900X
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center, University of Amsterdam, Amsterdam 1066CX, the 
      Netherlands; lgutierrezg@salud.madrid.org.
AD  - Departamento de Hematologia, Hospital Clinico San Carlos, Instituto de 
      Investigacion Sanitaria San Carlos, Madrid 28040, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161104
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Ccl19 protein, mouse)
RN  - 0 (Chemokine CCL19)
RN  - 0 (Chemokine CCL21)
RN  - 0 (GATA1 Transcription Factor)
RN  - 0 (Gata1 protein, mouse)
RN  - 0 (Sialic Acids)
RN  - 0 (polysialic acid)
SB  - IM
MH  - Animals
MH  - Cell Movement/genetics/*immunology
MH  - Chemokine CCL19/genetics/immunology
MH  - Chemokine CCL21/genetics/*immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - GATA1 Transcription Factor/deficiency/*immunology
MH  - Lymph Nodes/cytology/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Sialic Acids/genetics/*immunology
EDAT- 2016/11/07 06:00
MHDA- 2017/07/27 06:00
CRDT- 2016/11/06 06:00
PHST- 2016/01/21 00:00 [received]
PHST- 2016/09/29 00:00 [accepted]
PHST- 2016/11/07 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2016/11/06 06:00 [entrez]
AID - jimmunol.1600103 [pii]
AID - 10.4049/jimmunol.1600103 [doi]
PST - ppublish
SO  - J Immunol. 2016 Dec 1;197(11):4312-4324. doi: 10.4049/jimmunol.1600103. Epub 2016 
      Nov 4.