PMID- 27815464 OWN - NLM STAT- MEDLINE DCOM- 20180129 LR - 20191113 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 30 IP - 6 DP - 2016 11-12 TI - Retrograde Oxygen Persufflation of Kidney - Experiment on an Animal. PG - 801-805 AB - AIM: There is still a lack of organs for transplantation purposes. In the field of kidney and liver transplantation, one available solution is the use of organs from so-called marginal donors. These donors can be e.g. non-heart-beating donors. In these cases, perfusion and preservation of organs intended for transplantation is generally more difficult. Retrograde oxygen persufflation (ROP) may be a possible solution to this issue. This method is based on retrograde perfusion by oxygen through the renal vein thus reconditioning the organ. MATERIALS AND METHODS: We operated on 10 animals (porcine models). Ischemic injury of the right kidney was simulated in all animals. In group A (N=5), kidneys were perfused with retrograde oxygen persufflation after explantation. In group B (N=5), kidneys were perfused intrarterially as in usual clinical practice. After perfusion all kidneys were transplanted to the original donor animal. Quality of graft restitution was evaluated by the urea level obtained from the renal vein and by histopathological analysis after explantation. RESULTS: We found no statistically significant differences between groups A and B in urea levels after transplantation, nor did we find any significant differences in quality of kidney parenchyma restoration between these groups. CONCLUSION: Retrograde oxygen persufflation is able to protect and restore kidney parenchyma. CI - Copyright (c) 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. FAU - Molacek, Jiri AU - Molacek J AD - Department of Vascular Surgery, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic molacek@fnplzen.cz. AD - Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic. FAU - Opatrny, Vaclav AU - Opatrny V AD - Department of Vascular Surgery, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic. FAU - Matejka, Roman AU - Matejka R AD - Department of Biomedical Technology, Faculty of Biomedical Engineering in Kladno, Czech Technical University in Prague, Kladno, Czech Republic. FAU - Baxa, Jan AU - Baxa J AD - Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic. FAU - Treska, Vladislav AU - Treska V AD - Department of Vascular Surgery, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic. AD - Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - S88TT14065 (Oxygen) SB - IM MH - Animals MH - Kidney/metabolism MH - Kidney Transplantation/*methods MH - Male MH - Organ Preservation/*methods MH - Oxygen/*metabolism MH - Perfusion/*methods MH - Portal Vein MH - Reperfusion Injury/metabolism MH - Sus scrofa MH - Transplantation, Autologous OTO - NOTNLM OT - Retrograde oxygen persifflation OT - kidney transplantation OT - non-heart-beating donor EDAT- 2016/11/07 06:00 MHDA- 2018/01/30 06:00 CRDT- 2016/11/06 06:00 PHST- 2016/05/08 00:00 [received] PHST- 2016/07/20 00:00 [accepted] PHST- 2016/11/06 06:00 [entrez] PHST- 2016/11/07 06:00 [pubmed] PHST- 2018/01/30 06:00 [medline] AID - 30/6/801 [pii] AID - 10.21873/invivo.10997 [doi] PST - ppublish SO - In Vivo. 2016 11-12;30(6):801-805. doi: 10.21873/invivo.10997.