PMID- 27816055
OWN - NLM
STAT- MEDLINE
DCOM- 20170817
LR  - 20231111
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 17
IP  - 1
DP  - 2016 Nov 5
TI  - Genome-wide profiling of chicken dendritic cell response to infectious bursal 
      disease.
PG  - 878
LID - 878
AB  - BACKGROUND: Avian infectious bursal disease virus (IBDV) is a highly contagious, 
      immunosuppressive disease of young chickens, which causes high mortality rates 
      and large economic losses in the poultry industry. Dendritic cells (DCs), which 
      are antigen-presenting cells, have the unique ability to induce both innate and 
      acquired immune responses and may significantly influence virus pathogenicity. To 
      understand the interaction between IBDV and DCs, a microarray was used to analyse 
      the response of DCs infected by IBDV. RESULTS: IBDV infection induced 479 
      upregulated and 466 downregulated mRNAs in chicken DCs. Analysis of Gene Ontology 
      suggested that transcription from the RNA polymerase II promoter and the RNA 
      biosynthetic process were enriched, and pathway analyses suggested that oxidative 
      phosphorylation, as well as the T cell receptor and Interleukin-17 (IL-17) 
      signalling pathways might be activated by IBDV infection. Moreover, microRNA 
      (miRNA) and long non-coding RNA (lncRNA) alterations in IBDV-infected chicken DCs 
      were observed. A total of 18 significantly upregulated or downregulated miRNAs 
      and 441 significantly upregulated or downregulated lncRNAs were identified in 
      IBDV-stimulated DCs. We constructed 42 transcription factor (TF)-miRNA-mRNA 
      interactions involving 1 TF, 3 miRNAs, and 42 mRNAs in IBDV-stimulated DCs. 
      Finally, we predicted the target genes of differentially expressed lncRNAs, and 
      constructed lncRNA-mRNA regulatory networks. CONCLUSIONS: The results of this 
      study suggest a mechanism to explain how IBDV infection triggers an effective 
      immune response in chicken DCs.
FAU - Lin, Jian
AU  - Lin J
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu, 210095, People's Republic of China.
FAU - Xia, Jing
AU  - Xia J
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu, 210095, People's Republic of China.
FAU - Zhang, Keyun
AU  - Zhang K
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu, 210095, People's Republic of China.
FAU - Yang, Qian
AU  - Yang Q
AD  - College of Life Science, Nanjing Agricultural University, Weigang 1, Nanjing, 
      Jiangsu, 210095, People's Republic of China. zxbyq@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161105
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Cluster Analysis
MH  - Computational Biology/methods
MH  - Dendritic Cells/*immunology/*metabolism
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Gene Regulatory Networks
MH  - *Genome-Wide Association Study
MH  - Infectious bursal disease virus/*immunology
MH  - MicroRNAs/genetics
MH  - Poultry Diseases/*genetics/*immunology/virology
MH  - RNA Interference
MH  - RNA, Long Noncoding/genetics
MH  - RNA, Messenger/genetics
MH  - Transcription Factors/metabolism
MH  - Transcriptome
PMC - PMC5097849
OTO - NOTNLM
OT  - Dendritic cells
OT  - Infectious bursal disease virus
OT  - lncRNA
OT  - miRNA
EDAT- 2016/11/07 06:00
MHDA- 2017/08/18 06:00
PMCR- 2016/11/05
CRDT- 2016/11/07 06:00
PHST- 2016/03/13 00:00 [received]
PHST- 2016/10/12 00:00 [accepted]
PHST- 2016/11/07 06:00 [entrez]
PHST- 2016/11/07 06:00 [pubmed]
PHST- 2017/08/18 06:00 [medline]
PHST- 2016/11/05 00:00 [pmc-release]
AID - 10.1186/s12864-016-3157-5 [pii]
AID - 3157 [pii]
AID - 10.1186/s12864-016-3157-5 [doi]
PST - epublish
SO  - BMC Genomics. 2016 Nov 5;17(1):878. doi: 10.1186/s12864-016-3157-5.