PMID- 27816225
OWN - NLM
STAT- MEDLINE
DCOM- 20170307
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1474
DP  - 2016 Nov 25
TI  - Multiresidue analysis of cephalosporin antibiotics in bovine milk based on 
      molecularly imprinted polymer extraction followed by liquid chromatography-tandem 
      mass spectrometry.
PG  - 121-129
LID - S0021-9673(16)31454-6 [pii]
LID - 10.1016/j.chroma.2016.10.069 [doi]
AB  - This work reports the preparation of molecularly imprinted polymers (MIPs) 
      selective to cephalosporin (CF) antibiotics, and their application as molecularly 
      imprinted solid-phase extraction (MISPE) sorbents for the determination of these 
      antimicrobials in milk samples. Several functional monomers and cross-linkers 
      have been screened to select the best combination that provides high selectivity 
      for the simultaneous multiresidue extraction of cefthiofur (THIO), cefazolin 
      (AZO), cefquinome (QUI), cephapirin (API), cephalexin (ALE) and cephalonium (ALO) 
      from the samples. The novel MIPs were prepared by a non-covalent imprinting 
      approach in the form of spherical microparticles using the synthetic surrogate 
      molecule sodium 7-(2-biphenylylcarboxamido)-3-methyl-3-cepheme-4-carboxylate, 
      N-3,5-bis(trifluoromethyl)phenyl-N'-4-vinylphenyl urea (VPU) as functional 
      monomer, and divinylbenzene (DVB) as crosslinking agent in a 1:2:20 molar ratio. 
      The optimized MISPE method allowed the extraction of the target antimicrobials 
      from raw cow milk samples using a selective washing with 5mL 
      methanol/2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) buffer 
      (0.1M, pH 7.5) (2:98, v/v) to remove the non-specifically retained compounds, 
      followed by elution with 1mL of trifluoroacetic acid (TFA) in methanol (0.1:99.9, 
      v/v). The extracts have been analysed by UHPLC-MS/MS and the analytical method 
      has been validated according to EU guideline 2002/657/EC. The limits of 
      quantification (S/N=10) were in the 1.7-12.5mugkg(-1) range, well below the 
      maximum residue limits (MRLs) currently established for the quantified 
      cephalosporins in milk samples. The developed MIP allows mutiresidual 
      determination of the six cephalosporin antibiotics mentioned above, significantly 
      broadening the application to food analysis of MISPE methods.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Baeza, A N
AU  - Baeza AN
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense 
      de Madrid, E-28040 Madrid, Spain; Institute of Materials Research and Engineering 
      (IMRE), University of Havana, Havana 10400, Cuba.
FAU - Urraca, J L
AU  - Urraca JL
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense 
      de Madrid, E-28040 Madrid, Spain; CEI Campus Moncloa, UCM-UPM, Avda Complutense 
      s/n, E-28040 Madrid, Spain. Electronic address: jurracar@quim.ucm.es.
FAU - Chamorro, R
AU  - Chamorro R
AD  - Department of Organic Chemistry, Faculty of Chemistry, Universidad Complutense de 
      Madrid, E-28040 Madrid, Spain.
FAU - Orellana, G
AU  - Orellana G
AD  - Department of Organic Chemistry, Faculty of Chemistry, Universidad Complutense de 
      Madrid, E-28040 Madrid, Spain.
FAU - Castellari, M
AU  - Castellari M
AD  - Institute for Food and Agricultural Research and Technology, Food Safety Program, 
      Granja Camps i Armet s/n, Monells 17121, Girona, Spain.
FAU - Moreno-Bondi, M C
AU  - Moreno-Bondi MC
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense 
      de Madrid, E-28040 Madrid, Spain. Electronic address: mcmbondi@quim.ucm.es.
LA  - eng
PT  - Journal Article
DEP - 20161029
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Cephalosporins)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Polymers)
RN  - 0 (Solvents)
RN  - E5R8Z4G708 (Trifluoroacetic Acid)
RN  - RWW266YE9I (HEPES)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Cattle
MH  - Cephalosporins/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Cross-Linking Reagents
MH  - Drug Residues/*analysis
MH  - HEPES
MH  - Methanol
MH  - Microscopy, Electron, Scanning
MH  - Milk/*chemistry
MH  - Molecular Imprinting
MH  - Polymers
MH  - Solvents
MH  - Tandem Mass Spectrometry
MH  - Trifluoroacetic Acid
OTO - NOTNLM
OT  - Cephalosporins
OT  - MISPE
OT  - Milk analysis
OT  - Molecularly imprinted polymers
EDAT- 2016/11/07 06:00
MHDA- 2017/03/08 06:00
CRDT- 2016/11/07 06:00
PHST- 2016/07/14 00:00 [received]
PHST- 2016/10/10 00:00 [revised]
PHST- 2016/10/27 00:00 [accepted]
PHST- 2016/11/07 06:00 [pubmed]
PHST- 2017/03/08 06:00 [medline]
PHST- 2016/11/07 06:00 [entrez]
AID - S0021-9673(16)31454-6 [pii]
AID - 10.1016/j.chroma.2016.10.069 [doi]
PST - ppublish
SO  - J Chromatogr A. 2016 Nov 25;1474:121-129. doi: 10.1016/j.chroma.2016.10.069. Epub 
      2016 Oct 29.