PMID- 27816647
OWN - NLM
STAT- MEDLINE
DCOM- 20170328
LR  - 20170328
IS  - 1873-2682 (Electronic)
IS  - 1011-1344 (Linking)
VI  - 165
DP  - 2016 Dec
TI  - Synthesis, characterization and anticancer effect of the ruthenium (II) 
      polypyridyl complexes on HepG2 cells.
PG  - 246-255
LID - S1011-1344(16)30844-2 [pii]
LID - 10.1016/j.jphotobiol.2016.10.038 [doi]
AB  - As one of the major cell regulated center, mitochondria are closely associated 
      with cell proliferation, apoptosis of tumor cell. In this work, four new 
      ruthenium (II) polypyridyl complexes [Ru(bpy)(2)(FTTP)](ClO(4))(2) (1) 
      (FTTP=11-(3-fluoro-naphthalen-2-yloxy)-4,5,9,14-tetraaza-benzo[b]triphenylene, 
      bpy=2,2'-bipyridine), [Ru(phen)(2)(FTTP)](ClO(4))(2) (2) 
      (phen=1,10-phenanthroline), [Ru(bpy)(2)(PTTP)](ClO(4))(2) (3) 
      (PTTP=2-phenoxy-1,4,8,9-tetraazatriphenylene) and [Ru(phen)(2)(PTTP)](ClO(4))(2) 
      (4) were synthesized and characterized by elemental analysis, ESI-MS, (1)H NMR 
      and (13)C NMR. The cytotoxic activity, ability of inhibiting cell invasion, cell 
      cycle arrest and apoptosis-inducing mechanism of these Ru(II) complexes have been 
      investigated in detail by MTT (3-(4,5-dimethylthiazole)-2,5-diphenyltetrazolium 
      bromide) method, invasion assay, comet assay as well as western blotting 
      techniques. Notably, complexes 1-4 displayed high cytotoxic activity against 
      liver carcinoma HepG2 cells and the IC(50) values of complexes 1-4 against HepG2 
      cells are 10.4+/-1.2, 9.3+/-0.6, 29.1+/-1.5 and 5.6+/-1.2muM, respectively. The comet 
      assay showed that the complexes can induce DNA damage. The acridine orange (AO) 
      and ethidium bromide (EB) staining method indicated that the complexes can cause 
      apoptosis in HepG2 cells. Further studies showed that complexes 1-4 caused cell 
      cycle arrest at G0/G1 phase and induced HepG2 cells apoptosis through a 
      ROS-mediated mitochondrial dysfunction pathway, which involved an increase in the 
      levels of reactive oxygen species (ROS), a decrease in the mitochondrial membrane 
      potential, activation of caspases and Bcl-2 family proteins.
CI  - Copyright A(c) 2016 Elsevier B.V. All rights reserved.
FAU - Wan, Dan
AU  - Wan D
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Lai, Shang-Hai
AU  - Lai SH
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Yang, Hui-Hui
AU  - Yang HH
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Tang, Bing
AU  - Tang B
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Zhang, Cheng
AU  - Zhang C
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Yin, Hui
AU  - Yin H
AD  - Department of Microbiology and Immunology, Guangdong Pharmaceutical University, 
      Guangzhou 510006, PR China. Electronic address: huiyin0103@163.com.
FAU - Zeng, Chuan-Chuan
AU  - Zeng CC
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China.
FAU - Liu, Yun-Jun
AU  - Liu YJ
AD  - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR 
      China; Guangdong Cosmetics Engineering & Technology Research Center, Guangzhou 
      510006, PR China. Electronic address: lyjche@163.com.
LA  - eng
PT  - Journal Article
DEP - 20161031
PL  - Switzerland
TA  - J Photochem Photobiol B
JT  - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN  - 0 (Pyridines)
RN  - 0 (Reactive Oxygen Species)
RN  - 7UI0TKC3U5 (Ruthenium)
SB  - IM
MH  - Apoptosis
MH  - Carbon-13 Magnetic Resonance Spectroscopy
MH  - DNA Damage
MH  - Hep G2 Cells
MH  - Humans
MH  - Membrane Potential, Mitochondrial
MH  - Proton Magnetic Resonance Spectroscopy
MH  - Pyridines/*chemistry
MH  - Reactive Oxygen Species/metabolism
MH  - Ruthenium/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Spectrophotometry, Ultraviolet
OTO - NOTNLM
OT  - Cell invasion
OT  - Cytotoxicity in vitro
OT  - Mitochondrial membrane potential
OT  - Reactive oxygen species
OT  - Ruthenium (II) polypyridyl complexes
OT  - Western blot analysis
EDAT- 2016/11/07 06:00
MHDA- 2017/03/30 06:00
CRDT- 2016/11/07 06:00
PHST- 2016/09/25 00:00 [received]
PHST- 2016/10/27 00:00 [accepted]
PHST- 2016/11/07 06:00 [pubmed]
PHST- 2017/03/30 06:00 [medline]
PHST- 2016/11/07 06:00 [entrez]
AID - S1011-1344(16)30844-2 [pii]
AID - 10.1016/j.jphotobiol.2016.10.038 [doi]
PST - ppublish
SO  - J Photochem Photobiol B. 2016 Dec;165:246-255. doi: 
      10.1016/j.jphotobiol.2016.10.038. Epub 2016 Oct 31.