PMID- 27820950
OWN - NLM
STAT- MEDLINE
DCOM- 20170606
LR  - 20220310
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 57
IP  - 14
DP  - 2016 Nov 1
TI  - The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal 
      Pigment Epithelium Cells.
PG  - 5945-5953
LID - 10.1167/iovs.16-20604 [doi]
AB  - PURPOSE: The purpose of this study was to clarify the interactions between 
      macrophages (MPs) and RPE cells in coculture systems to investigate the 
      functional plasticity of RPE cells. METHODS: Adherent peritoneal cells or murine 
      MP cell line Raw 264.7 was cocultured with primary RPE cells taken from C57BL/6 
      mice, with or without lipopolysaccharide (LPS) or TNF-alpha stimulation. The cytokine 
      levels of the culture supernatants (CSs) were then analyzed with the Bio-Plex 
      murine 23-Plex Panel Assay Kit (Bio-Rad Laboratories). Monocyte chemoattractant 
      protein-1 (MCP-1), IL-6, VEGF, and TNF-alpha in CS were further quantified by ELISA. 
      The expression profiles, in cocultures, of complement-associated genes, TNF-alpha, 
      and angiogenesis-associated genes were analyzed by quantitative real-time PCR. 
      RESULTS: The production of MCP-1, IL-6, and VEGF was synergistically elevated 
      when primary MPs or RAW264.7 cells and RPE cells were cocultured compared with 
      those derived from sole cultures of MPs and RPE cells. The synergistic effect was 
      confirmed without direct cell contact and was more prominent in the presence of 
      LPS or TNF-alpha. TNF-alpha production by MPs was suppressed by RPE cells. Coculture of 
      RPE cells with RAW264.7 cells increased the gene expression of C3, CFB, and VEGF 
      genes, whereas it reduced those of complement regulatory factors CFH, CD59, 
      clusterin, and TNF-alpha and antiangiogenic pigment epithelium-derived factor (PEDF). 
      CONCLUSIONS: Our findings indicate the presence of ingenious interactions between 
      MPs and RPE cells that forces the inflammation and complement activation in the 
      vicinity of RPE cells under pathologic conditions.
FAU - Yamawaki, Takahiro
AU  - Yamawaki T
AD  - Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, 
      Japan.
FAU - Ito, Eiko
AU  - Ito E
AD  - Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto 
      Prefectural University of Medicine, Kyoto, Japan.
FAU - Mukai, Atsushi
AU  - Mukai A
AD  - Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto 
      Prefectural University of Medicine, Kyoto, Japan.
FAU - Ueno, Morio
AU  - Ueno M
AD  - Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, 
      Japan.
FAU - Yamada, Jun
AU  - Yamada J
AD  - Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, 
      Japan.
FAU - Sotozono, Chie
AU  - Sotozono C
AD  - Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, 
      Japan.
FAU - Kinoshita, Shigeru
AU  - Kinoshita S
AD  - Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto 
      Prefectural University of Medicine, Kyoto, Japan.
FAU - Hamuro, Junji
AU  - Hamuro J
AD  - Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, 
      Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Coculture Techniques
MH  - Cytokines/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/*metabolism
MH  - Interleukin-6/metabolism
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Real-Time Polymerase Chain Reaction
MH  - Retinal Pigment Epithelium/cytology/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2016/11/08 06:00
MHDA- 2017/06/07 06:00
CRDT- 2016/11/08 06:00
PHST- 2016/11/08 06:00 [entrez]
PHST- 2016/11/08 06:00 [pubmed]
PHST- 2017/06/07 06:00 [medline]
AID - 2582822 [pii]
AID - 10.1167/iovs.16-20604 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2016 Nov 1;57(14):5945-5953. doi: 
      10.1167/iovs.16-20604.