PMID- 27821351
OWN - NLM
STAT- MEDLINE
DCOM- 20170201
LR  - 20181202
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 181
DP  - 2016 Dec
TI  - Cytochrome P450 1A mRNA in the guppy Phalloceros caudimaculatus and response to 
      beta-naphthoflavone and environmental samples.
PG  - 86-93
LID - S0166-445X(16)30300-9 [pii]
LID - 10.1016/j.aquatox.2016.10.023 [doi]
AB  - The cytochrome P450 1A (CYP1A) mRNA is induced by environmental contaminants such 
      as PAHs, PCBs and dioxins. The present study cloned the CYP1A transcript from the 
      guppy Phalloceros caudimaculatus, which represents a potential fish for 
      toxicological studies in South America. The newly identified CYP1A encodes a 
      protein with 521 amino acids that shared 96-70% identity with other fishes. The 
      characterization of organ- and time-dependent induction of CYP1A using RT-qPCR 
      was evaluated after waterborne exposure to beta-naphthoflavone (BNF; 1muM). The 
      minimum exposure time that elicited significant CYP1A induction was 1h for liver, 
      gill, gut, brain, anal fin and fingerlings; 2h for dorsal fin; and 4h for kidney 
      and tail fin. CYP1A tended to reach peak induction in the first few hours (4h-8h) 
      of experiment in most organs, although levels remained induced until the end of 
      the experiment (96h). Validation of CYP1A use in environmental sample was 
      performed by exposing P. caudimaculatus to elutriate made from sediment of three 
      streams located in adjacent areas of the Patos Lagoon Estuary (RS, Brazil). CYP1A 
      in liver, gills and anal fin was induced by elutriate made from urban (S1) and 
      industrial (S2) sites; and not induced by a reference site located 22 Km from 
      potential contaminant sources, suggesting that environmental contamination plays 
      a role in this induction. The results suggest that fins could be used for CYP1A 
      biomarker analysis and employed in non-lethal biopsy methods for environmental 
      monitoring. The responsiveness of the newly identified CYP1A to BNF and elutriate 
      indicates that the guppy P. caudimaculatus could be used for environmental 
      toxicology investigations in South American environments.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Ferreira, Roger Stacke
AU  - Ferreira RS
AD  - Instituto de Ciencias Biologicas (ICB), Universidade Federal do Rio Grande 
      (FURG), Rio Grande, RS 96203-900, Brazil; Programa de Pos-graduacao em Biologia 
      de Ambientes Aquaticos Continentais (PPGBAC), Universidade Federal do Rio Grande 
      (FURG), Rio Grande, RS 96203-900, Brazil.
FAU - Chivittz, Cintia da Cruz
AU  - Chivittz CD
AD  - Instituto de Ciencias Biologicas (ICB), Universidade Federal do Rio Grande 
      (FURG), Rio Grande, RS 96203-900, Brazil; Programa de Pos-graduacao em Biologia 
      de Ambientes Aquaticos Continentais (PPGBAC), Universidade Federal do Rio Grande 
      (FURG), Rio Grande, RS 96203-900, Brazil.
FAU - Santos, Guilherme Senna Dos
AU  - Santos GS
AD  - Instituto de Ciencias Biologicas (ICB), Universidade Federal do Rio Grande 
      (FURG), Rio Grande, RS 96203-900, Brazil.
FAU - Zanette, Juliano
AU  - Zanette J
AD  - Instituto de Ciencias Biologicas (ICB), Universidade Federal do Rio Grande 
      (FURG), Rio Grande, RS 96203-900, Brazil; Programa de Pos-graduacao em Biologia 
      de Ambientes Aquaticos Continentais (PPGBAC), Universidade Federal do Rio Grande 
      (FURG), Rio Grande, RS 96203-900, Brazil. Electronic address: 
      julianozanette@furg.br.
LA  - eng
PT  - Journal Article
DEP - 20161025
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Biomarkers)
RN  - 0 (Fish Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animal Fins/drug effects/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cytochrome P-450 CYP1A1/chemistry/genetics/*metabolism
MH  - Environmental Monitoring
MH  - Fish Proteins/genetics/metabolism
MH  - Gills/drug effects/metabolism
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Poecilia/growth & development/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - Sequence Alignment
MH  - Water Pollutants, Chemical/chemistry/*toxicity
MH  - beta-Naphthoflavone/*toxicity
OTO - NOTNLM
OT  - Biomarker
OT  - CYP1A
OT  - Elutriate
OT  - Fish
OT  - Pollution
EDAT- 2016/11/09 06:00
MHDA- 2017/02/02 06:00
CRDT- 2016/11/09 06:00
PHST- 2016/07/25 00:00 [received]
PHST- 2016/10/20 00:00 [revised]
PHST- 2016/10/24 00:00 [accepted]
PHST- 2016/11/09 06:00 [pubmed]
PHST- 2017/02/02 06:00 [medline]
PHST- 2016/11/09 06:00 [entrez]
AID - S0166-445X(16)30300-9 [pii]
AID - 10.1016/j.aquatox.2016.10.023 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2016 Dec;181:86-93. doi: 10.1016/j.aquatox.2016.10.023. Epub 2016 
      Oct 25.