PMID- 27821608
OWN - NLM
STAT- MEDLINE
DCOM- 20170614
LR  - 20190329
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Print)
IS  - 0021-9193 (Linking)
VI  - 199
IP  - 2
DP  - 2017 Jan 15
TI  - Role of RNase Y in Clostridium perfringens mRNA Decay and Processing.
LID - 10.1128/JB.00703-16 [doi]
LID - e00703-16
AB  - RNase Y is a major endoribonuclease that plays a crucial role in mRNA degradation 
      and processing. We study the role of RNase Y in the Gram-positive anaerobic 
      pathogen Clostridium perfringens, which until now has not been well understood. 
      Our study implies an important role for RNase Y-mediated RNA degradation and 
      processing in virulence gene expression and the physiological development of the 
      organism. We began by constructing an RNase Y conditional knockdown strain in 
      order to observe the importance of RNase Y on growth and virulence. Our resulting 
      transcriptome analysis shows that RNase Y affects the expression of many genes, 
      including toxin-producing genes. We provide data to show that RNase Y depletion 
      repressed several toxin genes in C. perfringens and involved the virR-virS 
      two-component system. We also observe evidence that RNase Y is indispensable for 
      processing and stabilizing the transcripts of colA (encoding a major toxin 
      collagenase) and pilA2 (encoding a major pilin component of the type IV pili). 
      Posttranscriptional regulation of colA is known to be mediated by cleavage in the 
      5' untranslated region (5'UTR), and we observe that RNase Y depletion diminishes 
      colA 5'UTR processing. We show that RNase Y is also involved in the 
      posttranscriptional stabilization of pilA2 mRNA, which is thought to be important 
      for host cell adherence and biofilm formation. IMPORTANCE: RNases have important 
      roles in RNA degradation and turnover in all organisms. C. perfringens is a 
      Gram-positive anaerobic spore-forming bacterial pathogen that produces numerous 
      extracellular enzymes and toxins, and it is linked to digestive disorders and 
      disease. A highly conserved endoribonuclease, RNase Y, affects the expression of 
      hundreds of genes, including toxin genes, and studying these effects is useful 
      for understanding C. perfringens specifically and RNases generally. Moreover, 
      RNase Y is involved in processing specific transcripts, and we observed that this 
      processing in C. perfringens results in the stabilization of mRNAs encoding a 
      toxin and bacterial extracellular apparatus pili. Our study shows that RNase 
      activity is associated with gene expression, helping to determine the growth, 
      proliferation, and virulence of C. perfringens.
CI  - Copyright (c) 2016 American Society for Microbiology.
FAU - Obana, Nozomu
AU  - Obana N
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Ibaraki, Japan obana.nozomu.gb@u.tsukuba.ac.jp.
FAU - Nakamura, Kouji
AU  - Nakamura K
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Ibaraki, Japan.
FAU - Nomura, Nobuhiko
AU  - Nomura N
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 
      Ibaraki, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161228
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Bacterial Proteins)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.4.24.3 (Microbial Collagenase)
RN  - EC 3.4.24.3 (kappa-toxin, Clostridium perfringens)
SB  - IM
MH  - Bacterial Proteins/genetics/metabolism
MH  - Cell Proliferation
MH  - Clostridium perfringens/*enzymology/genetics/metabolism
MH  - Gene Expression Regulation, Bacterial/*physiology
MH  - Microbial Collagenase/genetics/metabolism
MH  - RNA, Bacterial/genetics/*metabolism
MH  - RNA, Messenger/genetics/*metabolism
MH  - Ribonucleases/genetics/*metabolism
PMC - PMC5198488
OTO - NOTNLM
OT  - Clostridium perfringens
OT  - RNase Y
OT  - posttranscriptional regulation
OT  - ribonuclease
OT  - toxin gene
OT  - toxin regulation
OT  - type IV pili
EDAT- 2016/11/09 06:00
MHDA- 2017/06/15 06:00
PMCR- 2017/07/15
CRDT- 2016/11/09 06:00
PHST- 2016/09/26 00:00 [received]
PHST- 2016/10/27 00:00 [accepted]
PHST- 2016/11/09 06:00 [pubmed]
PHST- 2017/06/15 06:00 [medline]
PHST- 2016/11/09 06:00 [entrez]
PHST- 2017/07/15 00:00 [pmc-release]
AID - JB.00703-16 [pii]
AID - 00703-16 [pii]
AID - 10.1128/JB.00703-16 [doi]
PST - epublish
SO  - J Bacteriol. 2016 Dec 28;199(2):e00703-16. doi: 10.1128/JB.00703-16. Print 2017 
      Jan 15.