PMID- 27822714
OWN - NLM
STAT- MEDLINE
DCOM- 20190508
LR  - 20210707
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Linking)
VI  - 54
IP  - 9
DP  - 2017 Nov
TI  - TWEAK-Fn14 Influences Neurogenesis Status via Modulating NF-kappaB in Mice with 
      Spinal Cord Injury.
PG  - 7497-7506
LID - 10.1007/s12035-016-0248-x [doi]
AB  - The aim of our research is to investigate the regulatory role of TNF-like weak 
      inducer of apoptosis- fibroblast growth factor-inducible 14 (TWEAK-Fn14) pathway 
      in nuclear factor-kappa B (NF-kappaB) expression and neurogenesis status after spinal 
      cord injury (SCI). We constructed a mice model of spinal cord injury and injected 
      different lentiviral vectors which were transfected with TWEAK, TWEAK small 
      interfering RNA (siRNA) and Fn14 siRNA into different groups of mice. Locomotor 
      functional recovery status of the hind limb in mice was assessed using the Basso, 
      Beattie and Bresnahan (BBB) test. Apoptosis status in the injured area was 
      examined via TDT-mediated dUTP-biotin nick end-labeling (TUNEL) staining, the 
      expression of GAP-43 in injured spinal cord was quantified by 
      immunohistochemistry and expressions of TWEAK, Fn14, NF-kappaB, TNF-alpha, and IL-1beta were 
      evaluated by either western blot or ELISA. The expressions of TWEAK, Fn14, and 
      NF-kappaB in the model group were significantly higher compared with those in the 
      control group. Furthermore, the TWEAK group in which TWEAK was overexpressed 
      exhibited significantly higher expressions of TWEAK, Fn14, and NF-kappaB, TNF-alpha and 
      IL-1beta in relation to those in the model group (P < 0.05 for all). Moreover, the 
      transfection of Fn14 siRNA antagonized the above effect of TWEAK transfection on 
      injured mice. On the other hand, the TWEAK siRNA group in which the expression of 
      TWEAK was inhibited exhibited significantly lower expressions of TWEAK, Fn14, 
      NF-kappaB, TNF-alpha, and IL-1beta (P < 0.05 for all). Moreover, the transfection of TWEAK 
      siRNA enhanced the locomotor functional recovery status in injured mice and 
      suppressed the apoptosis of injured areas (P < 0.05 for all). In conclusion, 
      stimulating the TWEAK-Fn14 pathway may elevate the expression of NF-kappaB, thereby 
      slow the function recovery of SCI mice whereas inhibiting the TWEAK-Fn14 pathway 
      may improve the neurogenesis status in mice with spinal cord injuries.
FAU - Xu, Jing
AU  - Xu J
AD  - Department of Otolaryngology-Head and Neck Surgery, Xiang-Ya Hospital, Central 
      South University, Changsha, Hunan, 410008, China.
AD  - Otolaryngology Key Laboratory of Hunan Province, Changsha, Hunan, 410008, China.
FAU - He, Jian
AU  - He J
AD  - Department of Otolaryngology-Head and Neck Surgery, Xiang-Ya Hospital, Central 
      South University, Changsha, Hunan, 410008, China.
AD  - Otolaryngology Key Laboratory of Hunan Province, Changsha, Hunan, 410008, China.
FAU - He, Huang
AU  - He H
AD  - Department of Neurosurgery, Xiang-Ya Hospital, Central South University, No. 87 
      Xiangya Road, Changsha, Hunan, 410008, China.
FAU - Peng, Renjun
AU  - Peng R
AD  - Department of Neurosurgery, Xiang-Ya Hospital, Central South University, No. 87 
      Xiangya Road, Changsha, Hunan, 410008, China.
FAU - Xi, Jian
AU  - Xi J
AD  - Department of Neurosurgery, Xiang-Ya Hospital, Central South University, No. 87 
      Xiangya Road, Changsha, Hunan, 410008, China. dr_shiqing@163.com.
LA  - eng
GR  - 81201740/National Natural Science Foundation of China/International
GR  - 2012FJ6075/Program Sponsored by the Science and Technology Department of Hunan 
      Province/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20161107
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - 0 (NF-kappa B)
RN  - 0 (TWEAK Receptor)
SB  - IM
RIN - Mol Neurobiol. 2021 Jul;58(7):3601. PMID: 33991321
MH  - Animals
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/*physiology
MH  - Neurogenesis/*physiology
MH  - Spinal Cord Injuries/*metabolism/pathology
MH  - TWEAK Receptor/antagonists & inhibitors/*biosynthesis
OTO - NOTNLM
OT  - Fn14
OT  - Gap-43
OT  - NF-kappaB
OT  - Spinal cord injury (SCI)
OT  - Tweak
EDAT- 2016/11/09 06:00
MHDA- 2019/05/09 06:00
CRDT- 2016/11/09 06:00
PHST- 2016/09/12 00:00 [received]
PHST- 2016/10/23 00:00 [accepted]
PHST- 2016/11/09 06:00 [pubmed]
PHST- 2019/05/09 06:00 [medline]
PHST- 2016/11/09 06:00 [entrez]
AID - 10.1007/s12035-016-0248-x [pii]
AID - 10.1007/s12035-016-0248-x [doi]
PST - ppublish
SO  - Mol Neurobiol. 2017 Nov;54(9):7497-7506. doi: 10.1007/s12035-016-0248-x. Epub 
      2016 Nov 7.