PMID- 27826112
OWN - NLM
STAT- MEDLINE
DCOM- 20170321
LR  - 20211020
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 59
DP  - 2016 Dec
TI  - Lipopolysaccharide-induced autophagy participates in the control of 
      pro-inflammatory cytokine release in grass carp head kidney leukocytes.
PG  - 389-397
LID - S1050-4648(16)30705-7 [pii]
LID - 10.1016/j.fsi.2016.11.010 [doi]
AB  - Microtubule-associated protein 1 light chain 3B (LC3B) is a known marker of 
      autophagy in mammals. In the present study, we isolated and identified grass carp 
      LC3B (gcLC3B) cDNA and found its inductive expression in response to bacterial 
      infection in vivo. To assess the occurrence of autophagy in immune response, the 
      role of gcLC3B as an autophagy marker in grass carp was characterized. 
      Accordingly, grass carp kidney cells (CIKs) with stable expression of GFP-gcLC3B 
      were established and GFP-gcLC3B puncta were counted under a confocal fluorescence 
      microscope. Results showed that starvation, a conventional inducer of autophagy, 
      significantly enhanced GFP-gcLC3B puncta number, indicating the existence of 
      gcLC3B-linked autophagy in fish cells. Moreover, a commercial antibody 
      recognizing gcLC3B and 3-methyladenine (3-MA) were validated in grass carp CIKs, 
      and used to evaluate autophagy in grass carp head kidney leukocytes (HKLs) in 
      response to LPS. Western blotting assay showed that LPS significantly induced the 
      conversion of gcLC3B protein, providing the evidence for autophagy induced by LPS 
      in fish immune cells. Importantly, autophagy inhibition by 3-MA enhanced grass 
      carp IL-1beta and TNF-alpha secretion, indicating the involvement of autophagy in 
      pro-inflammatory cytokine production. Besides, 3-MA could amplify LPS-induced 
      IL-1beta and TNF-alpha release, implying that autophagic induction may drive a mechanism 
      for controlling inflammatory response in fish. Thus, our data highlight the role 
      of autophagy in fish immunity and provide new insight into the mechanism for the 
      regulation of inflammation in fish.
CI  - Copyright A(c) 2016 Elsevier Ltd. All rights reserved.
FAU - Qin, Lei
AU  - Qin L
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Zhang, Shengnan
AU  - Zhang S
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Feng, Shiyu
AU  - Feng S
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Yin, Licheng
AU  - Yin L
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China. Electronic address: zhouhongzh@uestc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20161105
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Microtubule-Associated Proteins)
SB  - IM
MH  - Aeromonas hydrophila/physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Autophagy/drug effects/immunology
MH  - Carps/*immunology
MH  - Cell Line
MH  - Cytokines/immunology
MH  - DNA, Complementary/genetics/metabolism
MH  - Fish Diseases/*genetics/immunology/microbiology
MH  - Fish Proteins/chemistry/genetics/immunology/metabolism
MH  - Gram-Negative Bacterial Infections/genetics/immunology/microbiology/*veterinary
MH  - Head Kidney/*immunology
MH  - Immunity, Innate
MH  - Leukocytes/*immunology
MH  - Lipopolysaccharides/pharmacology
MH  - Microtubule-Associated Proteins/chemistry/genetics/metabolism
MH  - Phylogeny
MH  - Sequence Alignment/veterinary
OTO - NOTNLM
OT  - Autophagic induction
OT  - Cytokine release
OT  - Grass carp
OT  - Inflammatory regulation
OT  - LPS challenge
OT  - Leukocyte
EDAT- 2016/11/09 06:00
MHDA- 2017/03/23 06:00
CRDT- 2016/11/10 06:00
PHST- 2016/07/17 00:00 [received]
PHST- 2016/11/02 00:00 [revised]
PHST- 2016/11/03 00:00 [accepted]
PHST- 2016/11/09 06:00 [pubmed]
PHST- 2017/03/23 06:00 [medline]
PHST- 2016/11/10 06:00 [entrez]
AID - S1050-4648(16)30705-7 [pii]
AID - 10.1016/j.fsi.2016.11.010 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Dec;59:389-397. doi: 10.1016/j.fsi.2016.11.010. Epub 
      2016 Nov 5.