PMID- 27826230
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 10
DP  - 2016
TI  - AMPA Receptors Are Involved in Store-Operated Calcium Entry and Interact with 
      STIM Proteins in Rat Primary Cortical Neurons.
PG  - 251
LID - 251
AB  - The process of store-operated calcium entry (SOCE) leads to refilling the 
      endoplasmic reticulum (ER) with calcium ions (Ca(2+)) after their release into 
      the cytoplasm. Interactions between (ER)-located Ca(2+) sensors (stromal 
      interaction molecule 1 [STIM1] and STIM2) and plasma membrane-located Ca(2+) 
      channel-forming protein (Orai1) underlie SOCE and are well described in 
      non-excitable cells. In neurons, however, SOCE appears to be more complex because 
      of the importance of Ca(2+) influx via voltage-gated or ionotropic 
      receptor-operated Ca(2+) channels. We found that the SOCE inhibitors ML-9 and 
      SKF96365 reduced alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid 
      (AMPA)-induced [Ca(2+)](i) amplitude by 80% and 53%, respectively. To assess the 
      possible involvement of AMPA receptors (AMPARs) in SOCE, we used their specific 
      inhibitors. As estimated by Fura-2 acetoxymethyl (AM) single-cell Ca(2+) 
      measurements in the presence of CNQX or NBQX, thapsigargin (TG)-induced Ca(2+) 
      influx decreased 2.2 or 3.7 times, respectively. These results suggest that under 
      experimental conditions of SOCE when Ca(2+) stores are depleted, Ca(2+) can enter 
      neurons also through AMPARs. Using specific antibodies against STIM proteins or 
      GluA1/GluA2 AMPAR subunits, co-immunoprecipitation assays indicated that when 
      Ca(2+) levels are low in the neuronal ER, a physical association occurs between 
      endogenous STIM proteins and endogenous AMPAR receptors. Altogether, our data 
      suggest that STIM proteins in neurons can control AMPA-induced Ca(2+) entry as a 
      part of the mechanism of SOCE.
FAU - Gruszczynska-Biegala, Joanna
AU  - Gruszczynska-Biegala J
AD  - Laboratory of Neurodegeneration, International Institute of Molecular and Cell 
      Biology in Warsaw Warsaw, Poland.
FAU - Sladowska, Maria
AU  - Sladowska M
AD  - Laboratory of Neurodegeneration, International Institute of Molecular and Cell 
      Biology in Warsaw Warsaw, Poland.
FAU - Kuznicki, Jacek
AU  - Kuznicki J
AD  - Laboratory of Neurodegeneration, International Institute of Molecular and Cell 
      Biology in Warsaw Warsaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20161025
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC5078690
OTO - NOTNLM
OT  - AMPA receptors
OT  - STIM
OT  - calcium signaling
OT  - neurons
OT  - store-operated calcium entry (SOCE)
EDAT- 2016/11/09 06:00
MHDA- 2016/11/09 06:01
PMCR- 2016/01/01
CRDT- 2016/11/10 06:00
PHST- 2016/07/08 00:00 [received]
PHST- 2016/10/13 00:00 [accepted]
PHST- 2016/11/10 06:00 [entrez]
PHST- 2016/11/09 06:00 [pubmed]
PHST- 2016/11/09 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2016.00251 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2016 Oct 25;10:251. doi: 10.3389/fncel.2016.00251. 
      eCollection 2016.