PMID- 27827395
OWN - NLM
STAT- MEDLINE
DCOM- 20180516
LR  - 20240326
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Nov 9
TI  - Death receptor 5 (DR5) and a 5-gene apoptotic biomarker panel with significant 
      differential diagnostic potential in colorectal cancer.
PG  - 36532
LID - 10.1038/srep36532 [doi]
LID - 36532
AB  - High expression of Inhibitor of apoptosis proteins (IAPs) has been related to 
      colorectal cancer (CRC) progression, resistance to treatment and poor prognosis. 
      TRAIL (TNF-related apoptosis-inducing ligand) through its receptors DR4 
      (TRAIL-R1) and DR5 (TRAIL-R2) can selectively induce cancer cell apoptosis. The 
      mRNA expression of DR4, DR5, c-IAP1, c-IAP2, XIAP and BIRC5/Survivin genes was 
      examined in 100 paired (cancerous-normal) colorectal tissue specimens by 
      real-time PCR, 50 of which were KRAS wild-type and 50 KRAS-mutant. DR5, XIAP and 
      BIRC5/Survivin genes are significantly up-regulated (p < 0.0001, p = 0.012 and 
      p = 0.0003, respectively), whereas c-IAP1 and c-IAP2 genes are significantly 
      down-regulated at mRNA and protein levels in CRC (p < 0.0001 for both). ROC 
      analyses showed that DR5, cIAP1 and cIAP2 expression has discriminatory value 
      between CRC and normal tissue (AUC = 0.700, p < 0.0001 for DR5; AUC = 0.628, 
      p = 0.011 for cIAP1; AUC = 0.673, p < 0.0001 for cIAP2). Combinatorial ROC 
      analysis revealed the marginally fair discriminatory value of 5 genes as a panel 
      (AUC = 0.685, p < 0.0001). Kaplan-Meier survival curves revealed significant 
      association of cIAP2 down-regulation in CRC with lower overall survival 
      probability of CRC patients (p = 0.0098). DR5, BIRC5/Survivin, XIAP, c-IAP1 and 
      c-IAP2 mRNA expression are significantly deregulated in CRC and could provide a 
      panel of markers with significant discriminatory value between CRC and normal 
      colorectal tissue.
FAU - Devetzi, Marina
AU  - Devetzi M
AD  - Laboratory of Signal Mediated Gene Expression, Institute of Biology, Medicinal 
      Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, 
      Greece.
FAU - Kosmidou, Vivian
AU  - Kosmidou V
AD  - Laboratory of Signal Mediated Gene Expression, Institute of Biology, Medicinal 
      Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, 
      Greece.
FAU - Vlassi, Margarita
AU  - Vlassi M
AD  - Laboratory of Signal Mediated Gene Expression, Institute of Biology, Medicinal 
      Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, 
      Greece.
FAU - Perysinakis, Iraklis
AU  - Perysinakis I
AD  - 3rd Department of Surgery, General Hospital of Athens "G. Gennimatas", Athens, 
      Greece.
FAU - Aggeli, Chrysanthi
AU  - Aggeli C
AD  - 3rd Department of Surgery, General Hospital of Athens "G. Gennimatas", Athens, 
      Greece.
FAU - Choreftaki, Theodosia
AU  - Choreftaki T
AD  - Department of Pathology, General Hospital of Athens "G. Gennimatas", Athens, 
      Greece.
FAU - Zografos, Georgios N
AU  - Zografos GN
AD  - 3rd Department of Surgery, General Hospital of Athens "G. Gennimatas", Athens, 
      Greece.
FAU - Pintzas, Alexander
AU  - Pintzas A
AD  - Laboratory of Signal Mediated Gene Expression, Institute of Biology, Medicinal 
      Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, 
      Greece.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161109
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Survivin)
RN  - 0 (TNFRSF10B protein, human)
RN  - 0 (X-Linked Inhibitor of Apoptosis Protein)
RN  - 0 (XIAP protein, human)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Biomarkers, Tumor/*genetics
MH  - Colorectal Neoplasms/*diagnosis/genetics/metabolism
MH  - Down-Regulation
MH  - Gene Expression Profiling
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins/metabolism
MH  - RNA, Messenger/genetics
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/genetics/*metabolism
MH  - Survivin
MH  - Up-Regulation
MH  - X-Linked Inhibitor of Apoptosis Protein/metabolism
PMC - PMC5101514
EDAT- 2016/11/09 06:00
MHDA- 2018/05/17 06:00
PMCR- 2016/11/09
CRDT- 2016/11/10 06:00
PHST- 2016/02/18 00:00 [received]
PHST- 2016/10/13 00:00 [accepted]
PHST- 2016/11/10 06:00 [entrez]
PHST- 2016/11/09 06:00 [pubmed]
PHST- 2018/05/17 06:00 [medline]
PHST- 2016/11/09 00:00 [pmc-release]
AID - srep36532 [pii]
AID - 10.1038/srep36532 [doi]
PST - epublish
SO  - Sci Rep. 2016 Nov 9;6:36532. doi: 10.1038/srep36532.