PMID- 27827563
OWN - NLM
STAT- MEDLINE
DCOM- 20170308
LR  - 20171116
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 34
IP  - 4
DP  - 2017 Apr
TI  - Analytical strategies to detect enobosarm administration in bovines.
PG  - 632-640
LID - 10.1080/19440049.2016.1258122 [doi]
AB  - Selective androgen receptor modulators (SARMs) are a novel class of androgen 
      receptor ligands. They are intended to exhibit the same kind of effects as 
      androgenic drugs, like anabolic steroids, but be much more selective in their 
      action, targeting particular tissues without any undesirable effects on others. 
      While the main applications of these synthetic substances are for therapeutic 
      purposes, they also have a high potential for misuse in veterinary practice and 
      the sporting world. In order to guarantee for consumers with food from animal 
      origin that it is free from any residues of such compounds, analytical strategies 
      are required to ensure safe food and also to enable fair trade between producers. 
      In this context an animal experiment involving bovines administered with 
      enobosarm was conducted to provide the study with biological matrices. Different 
      animal matrices (urine and faeces) were investigated to select the most 
      appropriate matrix for use for control purposes, in terms of metabolite relevance 
      and detection time window. Based on ultra-high-pressure liquid chromatography 
      (UHPLC) with tandem mass spectrometry (LC-MS/MS) this work highlighted the 
      presence of sulfonated and glucuronated-conjugated forms of the molecule in the 
      urine of treated animals. Enobosarm could be detected in urine up to 9 days after 
      the administration when samples underwent phase II hydrolysis. Faeces was 
      demonstrated to be the main matrix of excretion of enobosarm since values up to 
      500 times higher compared with urine could be detected for 21 days. There was no 
      difference between the kinetic profiles when a deconjugation step was or was not 
      was applied.
FAU - Cesbron, Nora
AU  - Cesbron N
AD  - a LaBoratoire d'Etude des Residus et Contaminants dans les Aliments (LABERCA) , 
      Oniris , Nantes , France.
FAU - Sydor, Alexandre
AU  - Sydor A
AD  - a LaBoratoire d'Etude des Residus et Contaminants dans les Aliments (LABERCA) , 
      Oniris , Nantes , France.
FAU - Penot, Mylene
AU  - Penot M
AD  - a LaBoratoire d'Etude des Residus et Contaminants dans les Aliments (LABERCA) , 
      Oniris , Nantes , France.
FAU - Prevost, Stephanie
AU  - Prevost S
AD  - a LaBoratoire d'Etude des Residus et Contaminants dans les Aliments (LABERCA) , 
      Oniris , Nantes , France.
FAU - Le Bizec, Bruno
AU  - Le Bizec B
AD  - a LaBoratoire d'Etude des Residus et Contaminants dans les Aliments (LABERCA) , 
      Oniris , Nantes , France.
FAU - Dervilly-Pinel, Gaud
AU  - Dervilly-Pinel G
AD  - a LaBoratoire d'Etude des Residus et Contaminants dans les Aliments (LABERCA) , 
      Oniris , Nantes , France.
LA  - eng
PT  - Journal Article
DEP - 20161128
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Anabolic Agents)
RN  - 0 (Androgens)
RN  - 0 (Anilides)
RN  - 0 (Glucuronates)
RN  - 0 (Sulfones)
RN  - O3571H3R8N (ostarine)
SB  - IM
MH  - Anabolic Agents/pharmacokinetics/*urine
MH  - Androgens/pharmacokinetics/*urine
MH  - Anilides/pharmacokinetics/*urine
MH  - Animals
MH  - Biotransformation
MH  - Cattle
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues/*analysis/metabolism
MH  - Drug and Narcotic Control
MH  - Feces/chemistry
MH  - Food Contamination/analysis
MH  - Glucuronates/urine
MH  - Humans
MH  - Substance Abuse Detection/*methods
MH  - Sulfones/urine
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - LC-MS/MS
OT  - SARMs
OT  - Selective androgen receptor modulator
OT  - faeces
OT  - growth factor
OT  - growth promoter
OT  - metabolism
OT  - urine
EDAT- 2016/11/10 06:00
MHDA- 2017/03/09 06:00
CRDT- 2016/11/10 06:00
PHST- 2016/11/10 06:00 [pubmed]
PHST- 2017/03/09 06:00 [medline]
PHST- 2016/11/10 06:00 [entrez]
AID - 10.1080/19440049.2016.1258122 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017 
      Apr;34(4):632-640. doi: 10.1080/19440049.2016.1258122. Epub 2016 Nov 28.