PMID- 27830149
OWN - NLM
STAT- MEDLINE
DCOM- 20170201
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2016
DP  - 2016
TI  - Synergistic Effects of Danshen (Salvia Miltiorrhiza Radix et Rhizoma) and Sanqi 
      (Notoginseng Radix et Rhizoma) Combination in Inhibiting Inflammation Mediators 
      in RAW264.7 Cells.
PG  - 5758195
LID - 5758195
AB  - Aims. This study aims to investigate the possible synergistic interactions of the 
      Danshen-Sanqi combination on vascular disease via their anti-inflammatory 
      activities. Methods. Nine combination ratios of Danshen-Sanqi extracts were 
      screened in the RAW264.7 cell line and their anti-inflammatory effects were 
      examined in lipopolysaccharide- (LPS-) induced nitric oxide (NO), tumor necrosis 
      factor (TNF), and monocyte chemoattractant protein-1 (MCP-1) generation pathways. 
      The interaction between Danshen and Sanqi on each target was analysed using 
      combination index (CI) and isobologram models. Additionally, the 
      anti-inflammatory activities of key bioactive compounds from Danshen and Sanqi 
      were tested using the same models. The compounds from each herb that exerted the 
      most potent activity were combined to evaluate their possible 
      synergistic/antagonistic interactions. Results. Danshen-Sanqi 8 : 2 was found to 
      be the optimal ratio and exerted a synergistic effect in inhibiting NO, TNF, and 
      MCP-1 when the concentrations were higher than 1.24, 1.89, and 2.17 mg/mL, 
      respectively. Although dihydrotanshinone I (DT) and ginsenoside Rd (Rd) from 
      Danshen and Sanqi, respectively, exhibited the greatest individual bioactivity in 
      the assays, antagonistic effects were observed for the DT-Rd combination 7 : 3. 
      Conclusion. This study provided scientific evidence to support the traditional 
      use of the Danshen-Sanqi combination for vascular disease through their 
      synergistic interactions on anti-inflammatory pathways.
FAU - Zhou, Xian
AU  - Zhou X
AUID- ORCID: 0000-0001-8766-8158
AD  - National Institute of Complementary Medicine (NICM), Western Sydney University, 
      Locked Bag 1797, Penrith, NSW 2751, Australia.
FAU - Razmovski-Naumovski, Valentina
AU  - Razmovski-Naumovski V
AD  - National Institute of Complementary Medicine (NICM), Western Sydney University, 
      Locked Bag 1797, Penrith, NSW 2751, Australia; South Western Sydney Clinical 
      School, Faculty of Medicine, University of New South Wales, Sydney, NSW, 
      Australia.
FAU - Chang, Dennis
AU  - Chang D
AUID- ORCID: 0000-0002-6584-738X
AD  - National Institute of Complementary Medicine (NICM), Western Sydney University, 
      Locked Bag 1797, Penrith, NSW 2751, Australia.
FAU - Li, Chunguang
AU  - Li C
AUID- ORCID: 0000-0002-7789-2209
AD  - National Institute of Complementary Medicine (NICM), Western Sydney University, 
      Locked Bag 1797, Penrith, NSW 2751, Australia.
FAU - Kam, Antony
AU  - Kam A
AD  - Molecular Biology, Nanyang Technological University, 50 Nanyang Avenue, Singapore 
      639798.
FAU - Low, Mitchell
AU  - Low M
AD  - National Institute of Complementary Medicine (NICM), Western Sydney University, 
      Locked Bag 1797, Penrith, NSW 2751, Australia.
FAU - Bensoussan, Alan
AU  - Bensoussan A
AUID- ORCID: 0000-0002-7433-667X
AD  - National Institute of Complementary Medicine (NICM), Western Sydney University, 
      Locked Bag 1797, Penrith, NSW 2751, Australia.
FAU - Chan, Kelvin
AU  - Chan K
AD  - National Institute of Complementary Medicine (NICM), Western Sydney University, 
      Locked Bag 1797, Penrith, NSW 2751, Australia; School of Pharmacy and 
      Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, 
      Byrom Street, Liverpool L3 3AF, UK; Faculty of Sciences, TCM Division, University 
      of Technology Sydney, Main Campus, P.O. Box 123, Broadway, NSW 2007, Australia.
LA  - eng
PT  - Journal Article
DEP - 20161018
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Cytokines)
RN  - 0 (Drug Combinations)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Immunologic Factors)
RN  - 0 (Inflammation Mediators)
RN  - 0 (sanqi)
SB  - IM
MH  - Animals
MH  - Cytokines/immunology
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Drug Synergism
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Immunity, Innate/*drug effects/*immunology
MH  - Immunologic Factors/immunology
MH  - Inflammation Mediators/*immunology
MH  - Mice
MH  - RAW 264.7 Cells
MH  - Salvia miltiorrhiza/*chemistry
MH  - Treatment Outcome
PMC - PMC5088307
EDAT- 2016/11/11 06:00
MHDA- 2017/02/02 06:00
PMCR- 2016/10/18
CRDT- 2016/11/11 06:00
PHST- 2016/07/17 00:00 [received]
PHST- 2016/09/21 00:00 [accepted]
PHST- 2016/11/11 06:00 [entrez]
PHST- 2016/11/11 06:00 [pubmed]
PHST- 2017/02/02 06:00 [medline]
PHST- 2016/10/18 00:00 [pmc-release]
AID - 10.1155/2016/5758195 [doi]
PST - ppublish
SO  - Biomed Res Int. 2016;2016:5758195. doi: 10.1155/2016/5758195. Epub 2016 Oct 18.