PMID- 27830561
OWN - NLM
STAT- MEDLINE
DCOM- 20180116
LR  - 20211204
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1506
DP  - 2017
TI  - Report on Liver Cell Transplantation Using Human Fetal Liver Cells.
PG  - 283-294
AB  - In an era of organ shortage, human fetuses donated after medically indicated 
      abortion could be considered a potential liver donor for hepatic cell isolation. 
      We investigated transplantation of fetal liver cells as a strategy to support 
      liver functionality in end-stage liver disease. Here, we report our protocol of 
      human fetal liver cells (hFLC) isolation in fetuses from 17 to 22 gestational 
      weeks, and our clinical procedure of hFLC transplantation through the splenic 
      artery.
FAU - Pietrosi, Giada
AU  - Pietrosi G
AD  - Hepatology Unit, Department of Medicine, Mediterranean Institute for 
      Transplantation and Advanced Specialized Therapies, IRCCS-ISMETT, Palermo, Italy. 
      gpietrosi@ismett.edu.
FAU - Chinnici, Cinzia
AU  - Chinnici C
AD  - Fondazione Ri.MED, Regenerative Medicine and Biomedical Technologies Unit, 
      Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT, 
      Palermo, Italy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Immunosuppressive Agents)
RN  - WM0HAQ4WNM (Tacrolimus)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Separation/instrumentation/*methods
MH  - Cell Transplantation/adverse effects/ethics/*methods/standards
MH  - End Stage Liver Disease/*therapy
MH  - Female
MH  - Fetal Tissue Transplantation/adverse effects/ethics/*methods/standards
MH  - Fetus/*cytology
MH  - Graft Rejection/prevention & control
MH  - Hepatocytes/immunology/microbiology/*transplantation
MH  - Humans
MH  - Immunosuppression Therapy/methods
MH  - Immunosuppressive Agents/therapeutic use
MH  - Liver/cytology
MH  - Perfusion/instrumentation/methods
MH  - Practice Guidelines as Topic
MH  - Pregnancy
MH  - Quality Control
MH  - Splenic Artery/surgery
MH  - Tacrolimus/therapeutic use
MH  - Tissue Donors
MH  - Tissue and Organ Harvesting/ethics/methods/standards
OTO - NOTNLM
OT  - Chronic liver disease
OT  - Collagenase perfusion
OT  - Fetal liver
OT  - Fetal stem cell transplantation
OT  - Liver cell transplantation
OT  - Liver progenitors
OT  - Liver stem cell isolation
EDAT- 2016/11/11 06:00
MHDA- 2018/01/18 06:00
CRDT- 2016/11/11 06:00
PHST- 2016/11/11 06:00 [entrez]
PHST- 2016/11/11 06:00 [pubmed]
PHST- 2018/01/18 06:00 [medline]
AID - 10.1007/978-1-4939-6506-9_20 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1506:283-294. doi: 10.1007/978-1-4939-6506-9_20.