PMID- 27831646
OWN - NLM
STAT- MEDLINE
DCOM- 20170807
LR  - 20211204
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 20
IP  - 20
DP  - 2016 Oct
TI  - Mechanism of feedback regulation of neutrophil inflammation in Henoch-Schonlein 
      purpura.
PG  - 4277-4285
LID - 11598 [pii]
AB  - OBJECTIVE: The aim of this study is to investigate the role of 
      complement-neutrophil feedback regulation of inflammatory response in 
      Henoch-Schonlein purpura (HSP) through constructing an animal model of HSP. 
      MATERIALS AND METHODS: Twenty-four SPF grade Japanese large-eared white rabbits 
      were randomly divided into normal group and model group, 12 for each group. HSP 
      model was constructed by challenging rabbits with gastric gavage of a decoction 
      solution containing ginger, Piper longum L. and pepper, intraperitoneal injection 
      of ovalbumin (OVA)-Freund's adjuvant and intravenous injection at marginal ear 
      vein and subcutaneous injection in the back of rabbits with OVA normal saline 
      solution. Changes in general conditions of rabbits including food intake, water 
      intake and body temperature as well as alterations in blood routine, urine 
      routine, reactive oxygen species (ROS), inflammatory cytokines and complement 
      were compared between two groups. In the meantime, N-Acetyl-L-Cysteine (NAC)and 
      hydrogen peroxide (H2O2) treatment was used to manipulate ROS level and 
      determined the changes in aforementioned parameters. RESULTS: After 
      sensitization, rabbits of the model group displayed significantly elevated body 
      temperature, apathy, reduced physical activity, significantly decreased water and 
      food intake compared to the situations before sensitization (p<0.05). Significant 
      pathological changes were observed in these rabbits through HE staining study. 
      Furthermore, blood levels of white blood cells (WBC), mean corpuscular hemoglobin 
      concentration (MCHC), neutrophils (NEU) and NEU% were significantly increased, 
      whereas levels of red blood cells (RBC), hemoglobin (HGB), eosinophils (EOS) and 
      EOS% were significantly decreased (p<0.05). No significant alterations were 
      observed in levels of mean corpuscular hemoglobin (MCH) and platelet (PLT) 
      (p>0.05). Urine with mucus and a strong odor was observed in model rabbits. 
      Proteinuria occurred in 66.67% of model rabbits, hematuria in 58.33% and presence 
      of WBC in the urine in 25%. Also, levels of ROS, inflammatory cytokines, tumor 
      growth factor (TGF)-beta, complement and tumor necrosis factor (TNF)-alpha were 
      significantly increased in model rabbits. After the treatment of ROS inhibitor, 
      NAC, levels of these parameters were significantly decreased (p<0.05), but 
      significantly increased after treatment of H2O2, the ROS agonist (p<0.05). 
      CONCLUSIONS: Complement-neutrophil feedback regulation of inflammatory response 
      plays important roles in the pathogenesis of HSP, and inhibition of ROS can 
      suppress the development and progression of HSP.
FAU - Wu, J-J
AU  - Wu JJ
AD  - Clinical Laboratory, Cangzhou Central Hospital, Hebei, China. hlxhu267@sina.com.
FAU - Zhu, Y-T
AU  - Zhu YT
FAU - Hu, Y-M
AU  - Hu YM
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Disease Progression
MH  - Hydrogen Peroxide
MH  - *IgA Vasculitis
MH  - *Inflammation
MH  - Neutrophils/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2016/11/11 06:00
MHDA- 2017/08/08 06:00
CRDT- 2016/11/11 06:00
PHST- 2016/11/11 06:00 [entrez]
PHST- 2016/11/11 06:00 [pubmed]
PHST- 2017/08/08 06:00 [medline]
AID - 11598 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2016 Oct;20(20):4277-4285.