PMID- 27833848
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2211-5463 (Print)
IS  - 2211-5463 (Electronic)
IS  - 2211-5463 (Linking)
VI  - 6
IP  - 11
DP  - 2016 Nov
TI  - Neutral ceramidase-enriched exosomes prevent palmitic acid-induced insulin 
      resistance in H4IIEC3 hepatocytes.
PG  - 1078-1084
AB  - Oversupply of free fatty acids such as palmitic acid (PA) from the portal vein 
      may cause liver insulin resistance. Production of reactive oxygen species plays a 
      pivotal role in PA-induced insulin resistance in H4IIEC3 hepatocytes. Recently, 
      we found that exosomes secreted from INS-1 cells that were transfected with 
      neutral ceramidase (NCDase) plasmids had raised NCDase activity; these 
      NCDase-enriched exosomes could inhibit PA-induced INS-1 cell apoptosis. Here, we 
      showed that PA reduced insulin-stimulated tyrosine phosphorylation of insulin 
      receptor substrate 2 and decreased insulin-stimulated uptake of the fluorescent 
      glucose analog 2-NBDG, confirming that insulin resistance occurred in PA-treated 
      H4IIEC3 cells. Moreover, NCDase-enriched exosomes from INS-1 cells rescued 
      PA-induced H4IIEC3 insulin resistance and blocked PA-induced reactive oxygen 
      species production in which ceramide was involved.
FAU - Zhu, Qun
AU  - Zhu Q
AD  - Department of Endocrinology the Second Affiliated Hospital of Nanjing Medical 
      University Jiangsu China; China-USA Lipids in Health and Disease Research Center 
      Guilin Medical University Guangxi China.
FAU - Zhu, Rongping
AU  - Zhu R
AD  - Laboratory of Hepatobiliary and Pancreatic Surgery Affiliated Hospital of Guilin 
      Medical University Guangxi China.
FAU - Jin, Junfei
AU  - Jin J
AD  - China-USA Lipids in Health and Disease Research Center Guilin Medical University 
      Guangxi China; Laboratory of Hepatobiliary and Pancreatic Surgery Affiliated 
      Hospital of Guilin Medical University Guangxi China; Guangxi Key Laboratory of 
      Molecular Medicine in Liver Injury and Repair Guilin Medical University Guangxi 
      China.
LA  - eng
PT  - Journal Article
DEP - 20161020
PL  - England
TA  - FEBS Open Bio
JT  - FEBS open bio
JID - 101580716
PMC - PMC5095145
OTO - NOTNLM
OT  - ceramide
OT  - exosome
OT  - insulin resistance
OT  - neutral ceramidase
OT  - palmitic acid
OT  - reactive oxygen species
EDAT- 2016/11/12 06:00
MHDA- 2016/11/12 06:01
PMCR- 2016/10/20
CRDT- 2016/11/12 06:00
PHST- 2016/06/20 00:00 [received]
PHST- 2016/08/25 00:00 [revised]
PHST- 2016/08/27 00:00 [accepted]
PHST- 2016/11/12 06:00 [entrez]
PHST- 2016/11/12 06:00 [pubmed]
PHST- 2016/11/12 06:01 [medline]
PHST- 2016/10/20 00:00 [pmc-release]
AID - FEB412125 [pii]
AID - 10.1002/2211-5463.12125 [doi]
PST - epublish
SO  - FEBS Open Bio. 2016 Oct 20;6(11):1078-1084. doi: 10.1002/2211-5463.12125. 
      eCollection 2016 Nov.