PMID- 27836761
OWN - NLM
STAT- MEDLINE
DCOM- 20170404
LR  - 20170404
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 101
DP  - 2016 Dec
TI  - Effect of Holarrhena antidysentrica (Ha) and Andrographis paniculata (Ap) on the 
      biofilm formation and cell membrane integrity of opportunistic pathogen 
      Salmonella typhimurium.
PG  - 76-82
LID - S0882-4010(16)30611-8 [pii]
LID - 10.1016/j.micpath.2016.11.001 [doi]
AB  - Increasing occurrence of gastroenteritis outbreaks caused by food borne 
      opportunistic microorganisms has become a major problem in food industry as well 
      as in immunocompromised host. Antimicrobial agents are losing their efficacy due 
      to increase in the microbial resistance. For such reasons, conventional treatment 
      has become limited to manage the infections state. Need of the hour is to 
      instigate the search for safer holistic alternatives. The present study was hence 
      conducted to assess the antibiofilm effect and mode of action of aquo alcoholic 
      extracts of Holarrhena antidysentrica (Ha) and Andrographis paniculata (Ap) 
      against the Salmonella enterica serovar typhimurium. Both the extracts were 
      screened for the presence of phytocompounds followed by the characterization 
      using Attenuated Total Reflection (ATR) infrared spectroscopy and bioactivity 
      finger print analysis. Anti-biofilm assays were determined to test the potential 
      of both extracts to inhibit the biofilm formation, while Propidium Iodide (PI) 
      uptake analysis revealed that cell membrane was damaged by the exposure of 
      nutraceuticals for 1 h. This study has demonstrated that both nutraceuticals have 
      anti-biofilm and antimicrobial activity perturbing the membrane integrity of 
      food-borne S. typhimurium and could be used as curative remedy to control the 
      food borne microbial infection.
CI  - Copyright A(c) 2016 Elsevier Ltd. All rights reserved.
FAU - Tanwar, Ankit
AU  - Tanwar A
AD  - Heavy Metal and Clinical Toxicology Laboratory, Department of Medical 
      Elementology and Toxicology, Hamdard University, Hamdard Nagar, New Delhi 110062, 
      India; Division of CBRN Defence, Institute of Nuclear Medicine and Allied 
      Sciences, Delhi, India.
FAU - Chawla, Raman
AU  - Chawla R
AD  - Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, 
      Delhi, India.
FAU - Chakotiya, Ankita Singh
AU  - Chakotiya AS
AD  - Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, 
      Delhi, India.
FAU - Thakur, Pallavi
AU  - Thakur P
AD  - Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, 
      Delhi, India.
FAU - Goel, Rajeev
AU  - Goel R
AD  - Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, 
      Delhi, India.
FAU - Basu, Mitra
AU  - Basu M
AD  - Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, 
      Delhi, India.
FAU - Arora, Rajesh
AU  - Arora R
AD  - Office of DG (LS), Defence Research and Development Organisation, DRDO Bhawan, 
      Delhi, India.
FAU - Khan, Haider Ali
AU  - Khan HA
AD  - Heavy Metal and Clinical Toxicology Laboratory, Department of Medical 
      Elementology and Toxicology, Hamdard University, Hamdard Nagar, New Delhi 110062, 
      India. Electronic address: hakhantox@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20161109
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
RN  - 36015-30-2 (Propidium)
SB  - IM
MH  - Andrographis/*chemistry
MH  - Anti-Bacterial Agents/isolation & purification/*pharmacology
MH  - Biofilms/*drug effects/growth & development
MH  - Cell Membrane/*drug effects/physiology
MH  - Holarrhena/*chemistry
MH  - Permeability/drug effects
MH  - Phytochemicals/isolation & purification/pharmacology
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Propidium/analysis
MH  - Salmonella typhimurium/*drug effects/physiology
MH  - Spectrum Analysis
MH  - Staining and Labeling
OTO - NOTNLM
OT  - Andrographis paniculata
OT  - Antibacterial
OT  - Antibiofilm
OT  - Holarrhena antidysentrica
OT  - Opportunistic pathogen
OT  - Salmonella typhimurium
EDAT- 2016/11/12 06:00
MHDA- 2017/04/05 06:00
CRDT- 2016/11/13 06:00
PHST- 2016/10/01 00:00 [received]
PHST- 2016/11/02 00:00 [revised]
PHST- 2016/11/02 00:00 [accepted]
PHST- 2016/11/12 06:00 [pubmed]
PHST- 2017/04/05 06:00 [medline]
PHST- 2016/11/13 06:00 [entrez]
AID - S0882-4010(16)30611-8 [pii]
AID - 10.1016/j.micpath.2016.11.001 [doi]
PST - ppublish
SO  - Microb Pathog. 2016 Dec;101:76-82. doi: 10.1016/j.micpath.2016.11.001. Epub 2016 
      Nov 9.