PMID- 27837634
OWN - NLM
STAT- MEDLINE
DCOM- 20170608
LR  - 20220316
IS  - 1107-0625 (Print)
IS  - 1107-0625 (Linking)
VI  - 21
IP  - 5
DP  - 2016 Sept-Oct
TI  - Elevated expression of matrix metalloproteinase-3 in human osteosarcoma and its 
      association with tumor metastasis.
PG  - 1279-1286
AB  - PURPOSE: Matrix metalloproteinase-3 (MMP-3) is one of the several MMPs that is 
      associated with malignant tumors of breast, colon, cervix and lung, where its 
      expression has been correlated with tumor invasion and metastasis. However, the 
      role of MMP-3 in metastasis of osteosarcoma has not yet been explored. METHODS: 
      MMP-3 expression in 15 primary and metastatic osteosarcomas with case-matched 
      adjacent non-tumor tissue was assessed by immunohistochemistry and quantitative 
      RT-PCR. Further, MMP-3 mRNA and protein levels were also determined in osteoblast 
      and osteosarcoma cell lines. Additionally, migration and invasion assays were 
      performed in MMP-3 knockdown cells. RESULTS: MMP-3 was expressed in 86.6% (13/15) 
      of the osteosarcoma patients and its expression was significantly higher in 
      metastatic tumors as compared to the primary osteosarcoma tumor tissues. 
      Furthermore, osteosarcoma cell lines showed higher MMP-3 expression as compared 
      to osteoblast cell lines. siRNA-mediated MMP-3 knockdown in osteosarcoma cell 
      lines significantly inhibited their migration and invasion properties. 
      CONCLUSION: Our results demonstrated that MMP-3 expression is deregulated in 
      osteosarcomas and this potentially contributes to metastasis and might be a 
      promising marker for the prognosis and therapy of metastatic osteosarcoma.
FAU - Huang, Jie-Feng
AU  - Huang JF
AD  - Department of Orthopedics, The first affiliated hospital of Zhejiang Chinese 
      Medical University, Hangzhou, Zhejiang Province 310006, China.
FAU - Du, Wen-Xi
AU  - Du WX
FAU - Chen, Jun-Jie
AU  - Chen JJ
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Bone Neoplasms/*enzymology/genetics/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Osteosarcoma/*enzymology/genetics/*secondary
MH  - RNA Interference
MH  - RNA, Messenger/genetics
MH  - Signal Transduction
MH  - Transfection
MH  - Up-Regulation
MH  - Young Adult
EDAT- 2016/11/13 06:00
MHDA- 2017/06/09 06:00
CRDT- 2016/11/13 06:00
PHST- 2016/11/13 06:00 [entrez]
PHST- 2016/11/13 06:00 [pubmed]
PHST- 2017/06/09 06:00 [medline]
PST - ppublish
SO  - J BUON. 2016 Sept-Oct;21(5):1279-1286.