PMID- 27837862
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR  - 20180917
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 162
DP  - 2017 Jan 1
TI  - Fluorescence polarisation immunoassays for strobilurin fungicides 
      kresoxim-methyl, trifloxystrobin and picoxystrobin.
PG  - 495-504
LID - S0039-9140(16)30814-1 [pii]
LID - 10.1016/j.talanta.2016.10.063 [doi]
AB  - Fluorescence polarisation immunoassays (FPIAs) based on monoclonal antibodies for 
      detection of three strobilurin fungicides - kresoxim-methyl (KM), trifloxystrobin 
      (TF) and picoxystrobin (PC), were developed and optimised. Fluorescein-labelled 
      derivatives of target antigens (tracers) were synthesised and purified by 
      thin-layer chromatography. Influence of tracer structures on the assay parameters 
      was investigated. For KM and TF, the best assay performance was achieved with the 
      homologous pairs of reagents. For the PC assay, the heterologous tracer, i.e. 
      fluorescein-labelled derivative of TF, was used. The developed FPIAs were applied 
      to the determination of KM, TF and PC in red wine. Most optimal sample 
      preparation was achieved with cross-linked poly(vinylpyrrolidone) as a sorbent. 
      This clean-up is simple, rapid and allows determination of all three strobilurin 
      fungicides in one sample. Detection limits of the developed FPIAs in red wine 
      were 28, 6 and 5ng/mL for KM, TF and PC, respectively. Recovery in spiked samples 
      averaged between 80% and 104%. Intra- and inter-assay coefficients of variance 
      were less than 12%. The developed FPIA methods can be applied to screening of 
      wine samples for KM, TF and PC residues without complicated cleanup.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Kolosova, Anna
AU  - Kolosova A
AD  - A.N. Bach Institute of Biochemistry, Federal Research Centre 'Fundamentals of 
      Biotechnology' of the Russian Academy of Sciences, Leninsky prospect 33, 119071 
      Moscow, Russia. Electronic address: anna_kolosova@hotmail.com.
FAU - Maximova, Ksenia
AU  - Maximova K
AD  - M.V. Lomonosov Moscow State University, Faculty of Chemistry, Department of 
      Chemical Enzymology, Leninsky Gory 1, 119991 Moscow, Russia.
FAU - Eremin, Sergei A
AU  - Eremin SA
AD  - M.V. Lomonosov Moscow State University, Faculty of Chemistry, Department of 
      Chemical Enzymology, Leninsky Gory 1, 119991 Moscow, Russia.
FAU - Zherdev, Anatoly V
AU  - Zherdev AV
AD  - A.N. Bach Institute of Biochemistry, Federal Research Centre 'Fundamentals of 
      Biotechnology' of the Russian Academy of Sciences, Leninsky prospect 33, 119071 
      Moscow, Russia.
FAU - Mercader, Josep V
AU  - Mercader JV
AD  - Department of Biotechnology, Institute of Agrochemistry and Food Technology, 
      Consejo Superior de Investigaciones Cientificas (IATA_CSIC), Agustin Escardino 7, 
      Paterna, 46980 Valencia, Spain.
FAU - Abad-Fuentes, Antonio
AU  - Abad-Fuentes A
AD  - Department of Biotechnology, Institute of Agrochemistry and Food Technology, 
      Consejo Superior de Investigaciones Cientificas (IATA_CSIC), Agustin Escardino 7, 
      Paterna, 46980 Valencia, Spain.
FAU - Dzantiev, Boris B
AU  - Dzantiev BB
AD  - A.N. Bach Institute of Biochemistry, Federal Research Centre 'Fundamentals of 
      Biotechnology' of the Russian Academy of Sciences, Leninsky prospect 33, 119071 
      Moscow, Russia.
LA  - eng
PT  - Journal Article
DEP - 20161017
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Acetates)
RN  - 0 (Fungicides, Industrial)
RN  - 0 (Imines)
RN  - 0 (Strobilurins)
RN  - 0LXZ062TTB (kresoxim-methyl)
RN  - 62DH7GEL1P (picoxystrobin)
RN  - F625Z36B2D (trifloxystrobin)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Acetates/analysis
MH  - Fluorescein/chemistry
MH  - Fluorescence Polarization Immunoassay/*methods
MH  - Fungicides, Industrial/*analysis
MH  - Imines/analysis
MH  - Reproducibility of Results
MH  - Strobilurins/*analysis
MH  - Wine/analysis
OTO - NOTNLM
OT  - Fluorescence polarisation immunoassay
OT  - Fungicide
OT  - Strobilurin
OT  - Tracer heterology
OT  - Wine
EDAT- 2016/11/14 06:00
MHDA- 2018/09/18 06:00
CRDT- 2016/11/14 06:00
PHST- 2016/07/27 00:00 [received]
PHST- 2016/10/11 00:00 [revised]
PHST- 2016/10/15 00:00 [accepted]
PHST- 2016/11/14 06:00 [entrez]
PHST- 2016/11/14 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
AID - S0039-9140(16)30814-1 [pii]
AID - 10.1016/j.talanta.2016.10.063 [doi]
PST - ppublish
SO  - Talanta. 2017 Jan 1;162:495-504. doi: 10.1016/j.talanta.2016.10.063. Epub 2016 
      Oct 17.