PMID- 27840397
OWN - NLM
STAT- MEDLINE
DCOM- 20170328
LR  - 20170328
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 27
IP  - 2
DP  - 2017 Feb 28
TI  - Characterization of Prototype Foamy Virus Infectivity in Transportin 3 Knockdown 
      Human 293t Cell Line.
PG  - 380-387
LID - 10.4014/jmb.1606.06011 [doi]
AB  - The foamy viruses are currently considered essential for development as vectors 
      for gene delivery. Previous studies demonstrated that prototype foamy virus (PFV) 
      can infect and replicate prevalently in a variety of cell types for its exclusive 
      replication strategy. However, the virus-host interaction, especially 
      PFV-transportin3 (TNPO3), is still poorly understood. In our investigation of the 
      role of TNPO3 in PFV infection, we found lower virus production in TNPO3 
      knockdown (KD) cells compared with wild-type 293T cells. PCR analysis revealed 
      that viral DNAs were mostly altered to circular forms: both 1-long terminal 
      repeat (1-LTR) and 2-LTR in TNPO3 KD cells. We therefore suggest that TNPO3 is 
      required for successful PFV replication, at least at/after the nuclear entry step 
      of viral DNA. These findings highlight the obscure mysteries of PFV-host 
      interaction and the requirement of TNPO3 for productive infection of PFV in 293T 
      cells.
FAU - Hamid, Faysal Bin
AU  - Hamid FB
AD  - Department of Systems Biotechnology, Chung-Ang University, Ansung 17546, Republic 
      of Korea.
FAU - Kim, Jinsun
AU  - Kim J
AD  - Department of Systems Biotechnology, Chung-Ang University, Ansung 17546, Republic 
      of Korea.
FAU - Shin, Cha-Gyun
AU  - Shin CG
AD  - Department of Systems Biotechnology, Chung-Ang University, Ansung 17546, Republic 
      of Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (DNA, Viral)
RN  - 0 (TNPO3 protein, human)
RN  - 0 (beta Karyopherins)
SB  - IM
MH  - DNA, Viral
MH  - Gene Knockdown Techniques
MH  - HEK293 Cells
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Spumavirus/genetics/*physiology
MH  - Terminal Repeat Sequences
MH  - Transfection
MH  - *Virus Replication
MH  - beta Karyopherins/genetics/*physiology
OTO - NOTNLM
OT  - 1-long terminal repeat
OT  - 293 T cell
OT  - Transportin-3
OT  - prototype foamy virus
EDAT- 2016/11/15 06:00
MHDA- 2017/03/30 06:00
CRDT- 2016/11/15 06:00
PHST- 2016/11/15 06:00 [pubmed]
PHST- 2017/03/30 06:00 [medline]
PHST- 2016/11/15 06:00 [entrez]
AID - 10.4014/jmb.1606.06011 [pii]
AID - 10.4014/jmb.1606.06011 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2017 Feb 28;27(2):380-387. doi: 10.4014/jmb.1606.06011.