PMID- 27840405
OWN - NLM
STAT- MEDLINE
DCOM- 20170220
LR  - 20190609
IS  - 2329-0358 (Electronic)
IS  - 1425-9524 (Linking)
VI  - 21
DP  - 2016 Nov 14
TI  - Effects of Reactive Oxygen Species on Differentiation of Bone Marrow Mesenchymal 
      Stem Cells.
PG  - 695-700
AB  - BACKGROUND The low differentiation rates for transplanted stem cells are 
      challenging problems in spinal cord injury (SCI) treatment. Studies have 
      demonstrated that the inhibition of the Notch1 pathway in bone marrow mesenchymal 
      stem cells (BMSCs) contributed to the differentiation of these cells. Research 
      findings that certain antioxidants induce BMSCs to differentiate into neuronal 
      cells suggest that BMSC differentiation is related to the level of reactive 
      oxygen species (ROS) in cells. This study aimed to define the effect of ROS on 
      the differentiation of BMSCs. MATERIAL AND METHODS In this study, after BMSCs 
      were induced with the antioxidant beta-mercaptoethanol (beta-ME), related proteins were 
      analyzed by Western blotting and immunofluorescence. In order to find the role of 
      ROS in the differentiation, DCFH-DA was used to detect ROS levels in 
      antioxidant-treated BMSCs, H2O2-treated BMSCs, and normal BMSCs, and the 
      expression levels of Notch1 and its downstream transcriptional suppressor Hes1 
      were analyzed. RESULTS Induced with beta-ME, Western blotting and immunofluorescence 
      revealed gradual increases in the expression of Nestin (a neural stem 
      cell-specific protein) and neuron-specific enolase (NSE) but decreases in Notch1 
      expression. The expression levels of Notch1 and Hes1 were positively correlated 
      with changes in ROS level. CONCLUSIONS These data suggest that the 
      antioxidant-induced differentiation of BMSCs into neurons may be related to 
      ROS-based regulation of the Notch1 signalling pathway.
FAU - Shi, Yao
AU  - Shi Y
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning, China (mainland).
FAU - Hu, Yiwen
AU  - Hu Y
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning, China (mainland).
FAU - Lv, Chen
AU  - Lv C
AD  - Department of Orthopaedic Surgery, The First Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China (mainland).
FAU - Tu, Guanjun
AU  - Tu G
AD  - Department of Orthopaedic Surgery, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20161114
PL  - United States
TA  - Ann Transplant
JT  - Annals of transplantation
JID - 9802544
RN  - 0 (Antioxidants)
RN  - 0 (Hes1 protein, rat)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptor, Notch1)
RN  - 0 (Transcription Factor HES-1)
RN  - 60-24-2 (Mercaptoethanol)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Bone Marrow Cells/*cytology/drug effects/metabolism
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cell Line
MH  - Cell Survival/drug effects/physiology
MH  - Mercaptoethanol/pharmacology
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/*metabolism
MH  - Receptor, Notch1/metabolism
MH  - Signal Transduction/drug effects/*physiology
MH  - Transcription Factor HES-1/metabolism
EDAT- 2016/11/15 06:00
MHDA- 2016/11/15 06:01
CRDT- 2016/11/15 06:00
PHST- 2016/11/15 06:00 [entrez]
PHST- 2016/11/15 06:00 [pubmed]
PHST- 2016/11/15 06:01 [medline]
AID - 900463 [pii]
AID - 10.12659/aot.900463 [doi]
PST - epublish
SO  - Ann Transplant. 2016 Nov 14;21:695-700. doi: 10.12659/aot.900463.