PMID- 27840777
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2162-8092 (Print)
IS  - 2162-8092 (Electronic)
IS  - 2162-8092 (Linking)
VI  - 3
IP  - 2
DP  - 2014 Apr
TI  - Minimizing Systematic Errors in Quantitative High Throughput Screening Data Using 
      Standardization, Background Subtraction, and Non-Parametric Regression.
LID - http://jes2s.com/may2014/pdfs/hts.pdf [pii]
AB  - Quantitative high throughput screening (qHTS) has the potential to transform 
      traditional toxicological testing by greatly increasing throughput and lowering 
      costs on a per chemical basis. However, before qHTS data can be utilized for 
      toxicity assessment, systematic errors such as row, column, cluster, and edge 
      effects in raw data readouts need to be removed. Normalization seeks to minimize 
      effects of systematic errors. Linear (LN) normalization, such as standardization 
      and background removal, minimizes row and column effects. Alternatively, local 
      weighted scatterplot smoothing (LOESS or LO) minimizes cluster effects. Both 
      approaches have been used to normalize large scale data sets in other contexts. A 
      new method is proposed in this paper to combine these two approaches (LNLO) to 
      account for systematic errors within and between experiments. Heat maps 
      illustrate that the LNLO method is more effective in removing systematic error 
      than either the LN or the LO approach alone. All analyses were performed on an 
      estrogen receptor agonist assay data set generated as part of the Tox21 
      collaboration.
FAU - Ray, Mitas
AU  - Ray M
AD  - William G. Enloe High School, 128 Clarendon Crescent, Raleigh, NC 27610.
FAU - Shockley, Keith
AU  - Shockley K
AD  - National Institute of Environmental Health Sciences (NIEHS/NIH), 111 TW Alexander 
      Dr., Research Triangle Park, NC 27709.
FAU - Kissling, Grace
AU  - Kissling G
AD  - National Institute of Environmental Health Sciences (NIEHS/NIH), 111 TW Alexander 
      Dr., Research Triangle Park, NC 27709.
LA  - eng
GR  - Z01 ES045003-11/Intramural NIH HHS/United States
GR  - ZIA ES102865/Intramural NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Exp Second Sci
JT  - The journal of experimental secondary science
JID - 101692418
PMC - PMC5102623
MID - NIHMS664110
EDAT- 2014/04/01 00:00
MHDA- 2014/04/01 00:01
PMCR- 2016/11/09
CRDT- 2016/11/15 06:00
PHST- 2016/11/15 06:00 [entrez]
PHST- 2014/04/01 00:00 [pubmed]
PHST- 2014/04/01 00:01 [medline]
PHST- 2016/11/09 00:00 [pmc-release]
AID - http://jes2s.com/may2014/pdfs/hts.pdf [pii]
PST - ppublish
SO  - J Exp Second Sci. 2014 Apr;3(2):http://jes2s.com/may2014/pdfs/hts.pdf.